1887

Abstract

SUMMARY

Confluent monolayers of BHK-21/C13 cells excreted increasing amounts of polyamines into the extracellular medium with time. Excretion was specifically of spermidine and was not the result of cell death or lysis. Herpes simplex virus type 1 (HSV-1) completely prevented excretion of polyamines from 2 h after infection of the cells. The virus specifically inhibited the release of free spermidine but not of conjugated polyamines.

Keyword(s): excretion , HSV-1 and spermidine
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-56-2-251
1981-10-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/56/2/JV0560020251.html?itemId=/content/journal/jgv/10.1099/0022-1317-56-2-251&mimeType=html&fmt=ahah

References

  1. Dion A. S., Herbst E. J. 1970; Polyamine changes during development of Drosophila melanogaster. Annals of theNew York Academy of Sciences 171:723–734
    [Google Scholar]
  2. Dulbecco R., Freeman G. 1959; Plaque production by polyoma virus. Virology 8:396–397
    [Google Scholar]
  3. Francke B. 1978; Cell free synthesis of herpes simplex virus DNA – the influence of polyamines. Biochemistry 17:5494–5499
    [Google Scholar]
  4. Gerner E. W., Russell D. H. 1977; The relationship between poly amine accumulation and DNA replication in synchronous Chinese hamster ovary cells after heat shock. Cancer Research 37:482–489
    [Google Scholar]
  5. Gibson W., Roizman B. 1971; Compartmentalization of spermine and spermidine in the herpes simplex virion. Proceedings of the National Academy of Sciences of the United States of America 68:2818–2821
    [Google Scholar]
  6. Gibson W., Roizman B. 1973; The structural and metabolic involvement of polyamines with herpes simplex virus. In Polyamines in Normal and Neoplastic Growth pp 123–135 Edited by Russell D. H. New York: Raven Press;
    [Google Scholar]
  7. Goldstein D. A., Heby O., Marton L. J. 1976; Biphasic stimulation of polyamine biosynthesis in primary mouse kidney cells by infection with polyoma virus: uncoupling from DNA and rRNA synthesis. Proceedings of the National Academy of Sciences of the United States of America 73:4022–4026
    [Google Scholar]
  8. Heby O., Marton L. J., Zardi L., Russell D. H., Baserga R. 1975; Changes in polyamine metabolism in WI-38 cells stimulated to proliferate. Experimental Cell Research 90:8–14
    [Google Scholar]
  9. Hodgson J., Williamson J. D. 1975; Ornithine decarboxylase activity in uninfected and vaccinia virus infected HeLa cells. Biochemical and Biophysical Research Communications 63:308–312
    [Google Scholar]
  10. Hogan B. L. M., Mcilwinney A., Murden S. 1974; Effects of growth conditions on the activity of ornithine decarboxylase in cultured hepatoma cells. II. Effect of serum and insulin. Journal of Cellular Physiology 83:353–358
    [Google Scholar]
  11. Hollta E. 1977; Oxidation of spermidine and spermine in rat liver. Purification and properties of polyamine oxidase. Biochemistry 16:91–100
    [Google Scholar]
  12. Isom H. C. 1979; Stimulation of ornithine decarboxylase by human cytomegalovirus. Journal of General Virology 42:265–278
    [Google Scholar]
  13. Janne J., Poso H., Raina A. 1978; Polyamines in rapid growth and cancer. Biochimica et Biophysica Acta 473:241–293
    [Google Scholar]
  14. Keir H. M., Gold E. 1963; Deoxyribonucleic acid nucleotidyl-transferase and deoxyribonuclease from cultured cells infected with herpes simplex virus. Biochimica et Biophysica Acta 72:263–276
    [Google Scholar]
  15. Lanzer W., Holowczak J. A. 1975; Polyamines in vaccinia virons and polypeptides released from viral cores by acid extraction. Journal of Virology 16:1254–1264
    [Google Scholar]
  16. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  17. McCormick F. 1978; Polyamine turnover and leakage during infection of HeLa and L cells with herpes simplex virus type 1. Virology 91:496–503
    [Google Scholar]
  18. Mccormick F., Newton A. A. 1975; Polyamine metabolism in cells infected with herpes simplex virus. Journal of General Virology 27:25–33
    [Google Scholar]
  19. Macpherson I. A., Stoker M. G. P. 1962; Polyoma transformation of hamster clones – an investigation of genetic factors affecting cell competence. Virology 16:147–151
    [Google Scholar]
  20. Melvin M. A. L., Keir H. M. 1978a; Polyamine metabolism in BHK-21/C13 cells: loss of spermidine from cells following transfer to serum-depleted medium. Experimental Cell Research 111:231–236
    [Google Scholar]
  21. Melvin M. A. L., Keir H. M. 1978b; Diminished excretion of polyamines from BHK-21/C13 cells exposed to methylglyoxal bis(guanylhydrazone). Biochemical Journal 174:349–352
    [Google Scholar]
  22. Melvin M. A. L., Keir H. M. 1980; Excretion of polyamines from mammalian cells in culture. In Polyamines in Biomedical Research pp 363–381 Edited by Gaugas J. M. London: John Wiley & Sons;
    [Google Scholar]
  23. Melvin M. A. L., Wallace H. M., Keir H. M. 1980; Conjugation of polyamines in mammalian cells in culture. In Physical Chemistry and Physics vol 12: pp 431–439 Edited by Doyle-Davis G. New York: Pacific Press;
    [Google Scholar]
  24. Pohjanpelto P. 1976; Putrescine transport is greatly increased in human fibroblasts initiated to proliferate. Journal of Cell Biology 68:512–520
    [Google Scholar]
  25. Roizman B. 1977; The herpesviruses. In Molecular Biology of Animal Viruses vol 2: pp 769–848 Edited by Nayak D. P. New York: Marcel Dekker;
    [Google Scholar]
  26. Russell W. C. 1962; A sensitive and precise assay for herpes virus. Nature, London 195:1028–1029
    [Google Scholar]
  27. Russell D. H., Snyder S. H. 1968; Amine synthesis in rapidly growing tissues: ornithine decarboxylase activity in regenerating rat liver, chick embryo and various tumours. Proceedings of the National Academy of Sciences of the United States of America 60:1420–1427
    [Google Scholar]
  28. Russell D. H., Medina V. J., Snyder S. H. 1970; The dynamics of synthesis and degradation of poly amines in normal and regenerating rat liver and brain. Journal of Biological Chemistry 245:6732–6738
    [Google Scholar]
  29. Schneider E. J., Stanbridge E. J., Epstein C. J. 1974; Incorporation of 3H-uridine and 3H-uracil into RNA. Detection of mycoplasma. Experimental Cell Research 84:311–318
    [Google Scholar]
  30. Tabor C. W., Tabor H. 1976; 1, 4-Diaminobutane (putrescine), spermidine and spermine. Annual Review of Biochemistry 45:285–306
    [Google Scholar]
  31. Tuomi K., Mantyjarvi R., Raina A. 1980; Inhibition of Semliki Forest virus and herpes simplex virus production in a-difluoromethylornithine-treated cells: reversal by polyamines. FEBS Letters 121:292–294
    [Google Scholar]
  32. Tyms A. S., Scamans E., Williamson J. D. 1979; Polyamine metabolism in MRC-5 cells infected with different herpes viruses. Biochemical and Biophysical Research Communications 86:312–318
    [Google Scholar]
  33. Wallace H. M., Melvin M. A. L., Keir H. M. 1979; Decreased excretion of polyamines from BHK-21/C13 cells treated with a-methylornithine. Biochemical Society Transactions 7:688–689
    [Google Scholar]
  34. Wallace H. M., Baybutt H. N., Pearson C. K., Keir H. M. 1980; The effect of polyamines on herpes simplex virus type 1 DNA polymerase purified from infected baby hamster kidney cells (BHK-21/C13). Journal of General Virology 49:397–400
    [Google Scholar]
  35. Williamson J. D. 1976; Effect of methylglyoxal bis(guanylhydrazone) on vaccinia virus replication. Biochemical and Biophysical Research Communications 73:120–126
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-56-2-251
Loading
/content/journal/jgv/10.1099/0022-1317-56-2-251
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error