1887

Abstract

SUMMARY

We investigated the ultrastructural development and maturation of cytomegalovirus (CMV) nuclear inclusions (NIs) in human embryo thyroid cells at 1 to 144 h post-infection. At 5 h, most cells had rounded from an initial fibroblastic appearance and contained early NIs. At 24 h, early NIs were larger and better defined. At 48 h, although early NIs were still present, most cells had larger and presumably more mature NIs. These latter NIs consisted of several subunits, each made up of a fibrillar network enclosing an electron-lucent area which contained coarse and delicate granules. Also, at 48 h, virus particles were first seen in the nucleoplasm. At 72 h, in cells with more developed NIs, virus particles were closely associated with the fibrillar network. Between 96 and 144 h, the NIs reached maximum size and were made up of numerous subunits. The results indicate that two types of NIs coexist during CMV infection. The appearance of the early and late NIs coincides with the reported peaks of CMV DNA synthesis and thus may explain the biphasic pattern of DNA synthesis in CMV infection. Morphogenetic features of the NIs conform with the hypothesis that synthesis of CMV DNA may occur in the centre of each NI subunit and that the fibrillar network represents condensing capsid proteins.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-56-1-97
1981-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/56/1/JV0560010097.html?itemId=/content/journal/jgv/10.1099/0022-1317-56-1-97&mimeType=html&fmt=ahah

References

  1. Albrecht T. B. 1973 Studies on the oncogenic potential of human cytomegalovirus Ph.D. thesis The Pennsylvania State University;
    [Google Scholar]
  2. Albrecht T., Rapp F. 1973; Malignant transformation of hamster embryo fibroblasts following exposure to ultraviolet-irradiated human cytomegalovirus. Virology 55:53–61
    [Google Scholar]
  3. Albrecht T., Weller T. H. 1980; Heterogeneous morphologic features of plaques induced by five strains of human cytomegalovirus. American Journal of Clinical Pathology 73:648–654
    [Google Scholar]
  4. Albrecht T., Cavallo T., Cole N. L., Graves K. 1980a; Cytomegalovirus: development and progression of cytopathic effects in human cell culture. Laboratory Investigation 42:1–7
    [Google Scholar]
  5. Albrecht T., Li M. L., Cole N., Downing E., Funk F. D. 1980b; Replication of human cytomegalovirus at supra-optimal temperature is dependent on the virus strain, multiplicity of infection, and phase of virus replication. Journal of General Virology 51:83–97
    [Google Scholar]
  6. Clyde W. A. Jr, Kim K. S. 1967; Biophysical characterization of human mycoplasma species. Annals of the New York Academy of Sciences 143:425–435
    [Google Scholar]
  7. Craighead J. E., Kanich R. E., Almeida J. D. 1972; Nonviral microbodies with viral antigenicity produced in cytomegalovirus-infected cells. Journal of Virology 10:766–775
    [Google Scholar]
  8. Furukawa T., Fioretti A., Plotkin S. 1973; Growth characteristics of cytomegalovirus in human fibroblasts with demonstration of protein synthesis early in viral replication. Journal of Virology 11:991–997
    [Google Scholar]
  9. Geder L. 1976; Evidence for early nuclear antigens in cytomegalovirus-infected cells. Journal of General Virology 32:315–319
    [Google Scholar]
  10. Goodheart C. R., McAllister R. M., Filbert J. E. 1964; Human cytomegalovirus: DNA synthesis and migration in infected cells studied autoradiographically. Virology 23:603–608
    [Google Scholar]
  11. Heine U., Kondratick J., Ablashi D. V., Armstrong G. R., Dalton A. J. 1971; UltraStTUCtUral and biological properties of a cytomegalovirus rescued from a human paraganglioma. Cancer Research 31:542–549
    [Google Scholar]
  12. Iwasaki Y., Furukawa T., Plotkin S., Koprowski H. 1973; Ultrastructural study on the sequence of human cytomegalovirus infection in human diploid cells. Archiv fur die gesamte Virusforschung 40:311–324
    [Google Scholar]
  13. Jesionek A., Kiolemenglou B. 1904; Ueber einen Befund von protozoenartigen Gebilden in den Organen eines hereditarluetischen Fotus. Miinchener medizinische Wochenschrift 51:1905–1907
    [Google Scholar]
  14. Kanich R. E., Craighead J. E. 1972; Human cytomegalovirus infection of cultured fibroblasts. I.Cytopathologic effects induced by an adapted and a wild strain. Laboratory Investigation 27:263–272
    [Google Scholar]
  15. Li J. L., Albrecht T. 1979; Acquisition of a transformed phenotype by human embryo lung cells persistently infected with human cytomegalovirus following exposure to 4-nitroquinoline 1-oxide (NQO). Abstracts of the Annual Meeting of the American Society for Microbiology p 301
    [Google Scholar]
  16. Luse S. A., Smith M. G. 1958; Electron microscopy of salivary gland viruses. Journal of Experimental Medicine 107:623–632
    [Google Scholar]
  17. McAllister R. M., Straw R. M., Filbert J. E., Goodheart C. R. 1963; Human cytomegalovirus: cytochemical observations of intracellular lesion development correlated with viral synthesis and release. Virology 19:521–531
    [Google Scholar]
  18. Mcgavran M. H., Smith M. G. 1965; Ultrastructural, cytochemical, and microchemical observations on cytomegalovirus (salivary gland virus) infection of human cells in tissue culture. Experimental and Molecular Pathology 4:1–10
    [Google Scholar]
  19. Martin A. M. Jr, Kurtz S. M. 1966; Cytomegalic inclusion disease: an electron microscopic histochemical study of the virus at necropsy. Archives of Pathology 82:27–34
    [Google Scholar]
  20. Mocarski E. S., Stinski M. F. 1979; Persistence of the cytomegalovirus genome in human cells. Journal of Virology 31:761–775
    [Google Scholar]
  21. Rapp F., Rasmussen L. E., Benyesh-Melnick M. 1963; The immunofluorescent focus technique in studying the replication of cytomegalovirus. Journal of Immunology 91:709–719
    [Google Scholar]
  22. Rapp F., Geder L., Murasko D., Lausch R., Ladda R., Huang E.-S., Webber M. M. 1975; Long-term persistence of cytomegalovirus genome in cultured human cells of prostatic origin. Journal of Virology 16:982–990
    [Google Scholar]
  23. Reynolds D. W. 1978; Development of early nuclear antigen in cytomegalovirus infected cells in the presence of RNA and protein synthesis inhibitors. Journal of General Virology 40:475–480
    [Google Scholar]
  24. Rowe W. P., Hartley J. W., Waterman S., Turner H. C., Huebner R. J. 1956; Cytopathogenic agent resembling human salivary gland virus recovered from tissue cultures of human adenoids. Proceedings of the Society for Experimental Biology and Medicine 92:418–424
    [Google Scholar]
  25. Ruebner B. H., Hirano T., Slusser R. J., Medearis D. N. Jr 1965; Human cytomegalovirus infection: electron microscopic and histochemical changes in cultures of human fibroblasts. American Journal of Pathology 46:477–496
    [Google Scholar]
  26. St. Jeor S. C., Hutt R. 1977; Cell DNA replication as a function in the synthesis of human cytomegalovirus. Journal of General Virology 37:65–73
    [Google Scholar]
  27. Sarov I., Abady I. 1975; The morphogenesis of human cytomegalovirus: isolation and polypeptide characterization of cytomegalovirions and dense bodies. Virology 66:464–473
    [Google Scholar]
  28. Sarov I., Abady I. 1977; Human cytomegalovirions and dense bodies: glycopeptide analysis and mechanism of cell rounding and polykaryocytosis. Israel Journal of Medical Science 13:887–895
    [Google Scholar]
  29. Smith M. G. 1956; Propagation in tissue cultures of a cytopathogenic virus from human salivary gland virus (SGV) disease. Proceedings of the Society for Experimental Biology and Medicine 92:424–430
    [Google Scholar]
  30. Smith M. G. 1959; The salivary gland viruses of man and animals (cytomegalic inclusion disease). Progress in Medical Virology 2:171–202
    [Google Scholar]
  31. Smith J. D., De Harven E. 1973; Herpes simplex virus and human cytomegalovirus replication in WI-38 cells. Sequence of viral replication. Journal of Virology 12:919–930
    [Google Scholar]
  32. Smith J. D., De Harven E. 1978; Herpes simplex virus and human cytomegalovirus replication in WI-38 cells.III. Cytochemical localization of lysosomal enzymes in infected cells. Journal of Virology 26:102–109
    [Google Scholar]
  33. Stinski M. F. 1976; Human cytomegalovirus: glycoproteins associated with virions and dense bodies. Journal of Virology 19:594–609
    [Google Scholar]
  34. Stinski M. F. 1978; Sequence of protein synthesis in cells infected by human cytomegalovirus: early and late virus-induced polypeptides. Journal of Virology 26:686–701
    [Google Scholar]
  35. Weller T. H. 1971; The cytomegaloviruses: ubiquitous agents with protean clinical manifestations. New England Journal of Medicine 285:203–214 267–274
    [Google Scholar]
  36. Weller T. H., Macauley J. C., Craig J. M., Wirth P. 1957; Isolation of intranuclear inclusion producing agents from infants with illnesses resembling cytomegalic inclusion disease. Proceedings of the Society for Experimental Biology and Medicine 94:4–12
    [Google Scholar]
  37. Wentworth B. B., French L. 1970; Plaque assay of cytomegalovirus strains of human origin. Proceedings of the Society for Experimental Biology and Medicine 135:253–258
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-56-1-97
Loading
/content/journal/jgv/10.1099/0022-1317-56-1-97
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error