1887

Abstract

SUMMARY

Restriction endonucleases RI, HI, dIII, I, II, I and I recognized 48 cleavage sites in EDS adenovirus strain B8/78 DNA, whereas CELO virus DNA was cut into 61 fragments by the same enzymes. No similarity could be detected in the restriction patterns obtained from the two avian adenoviruses. The calculated mol. wt. of B8/78 DNA was 22.6 × 10 [about 34.2 kilobases (kb)] and that of CELO virus DNA was 28.9 × 10 (about 43.7 kb). The fragments generated from B8/78 DNA by RI and HI were physically mapped.

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1981-09-01
2021-10-28
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References

  1. Adair B. M., Mcferran J. B., Connor T. J., Mcnulty M. S., Mckillop E. R. 1979; Biological and physical properties of a virus (strain 127) associated with the egg drop syndrome 1976. Avian Pathology 8:249–264
    [Google Scholar]
  2. Anonym 1977; Adenovirus strand nomenclature: a proposal. Journal of Virology 22:830–831
    [Google Scholar]
  3. Doerfler W., Kleinschmidt A. K. 1970; Denaturation pattern of the DNA of adenovirus type 2 as determined by electronmicroscopy. Journal of Molecular Biology 50:579–593
    [Google Scholar]
  4. Green M., Pina M., Rimes R., Wensink P. C., Machattie L. A., Thomas C. A. 1967; Adenovirus DNA. I. Molecular weight and confirmation. Proceedings of the National Academy of Sciences of the United States of America 57:1302–1309
    [Google Scholar]
  5. Kisary J., Zsák L. 1980; The molecular weight of egg drop syndrome (EDS) avian adenovirus DNA estimated by digestion with restriction endonuclease enzyme R. EcoRI . Archives of Virology 65:63–65
    [Google Scholar]
  6. Laver W. G., Younghusband H. B., Wrigley N. G. 1971; Purification and properties of chick embryo lethal orphan virus (an avian adenovirus). Virology 45:598–614
    [Google Scholar]
  7. McCracken R. M., Mcferran J. B. 1978; Experimental reproduction of the egg drop syndrome 1976 with a haemagglutinating adenovirus. Avian Pathology 7:483–490
    [Google Scholar]
  8. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. Journal of Molecular Biology 5:109–118
    [Google Scholar]
  9. Mulder C., Sharp P. A., Delius H., Pettersson U. 1974; Specific fragmentation of DNA by adenovirus serotypes 3, 5, 7 and 12, and adeno-simian virus to hybrid virus Ad2+ND1 by restriction endonuclease EcoRI . Journal of Virology 14:68–77
    [Google Scholar]
  10. Potter C. W., Oxford J. S., Downie J. C., Attwood M. M., Hardy R. D. 1971; Chick embryo lethal orphan (CELO) virus: some physical and immunological properties. Virology 44:418–424
    [Google Scholar]
  11. Robinson A. J., Younghusband H. B., Bellett A. J. D. 1973; A circular DNA-protein complex from adenoviruses. Virology 56:54–69
    [Google Scholar]
  12. Sharp P. A., Moore C., Haverty J. L. 1976; The infectivity of adenovirus 5 DNA-protein complex. Virology 75:442–456
    [Google Scholar]
  13. Tibbetts C., Johansson K., Philipson L. 1973; Hydroxyapatite chromatography and formamide denaturation of adenovirus DNA. Journal of Virology 12:218–225
    [Google Scholar]
  14. Todd D., Mcnulty M. S. 1978; Biochemical studies on a virus associated with egg drop syndrome 1976. Journal of General Virology 40:63–75
    [Google Scholar]
  15. Van Eck J. H. H., Davelaar F. G., Van Den Heuvel-Plesman T. A. A., Van Kol N., Kouwenhaven B., Guldie F. H. M. 1976; Dropped egg production, soft shelled and shell-less eggs associated with the appearance of precipitins to adenovirus in flocks of laying fowls. A vian Pathology 5:261–272
    [Google Scholar]
  16. Winberg G., Hammarskjöld M. L. 1980; Isolation of DNA from agarose gels using DEAE-paper. Application to restriction site mapping of adenovirus type 16 DNA. Nucleic Acids Research 8:253–264
    [Google Scholar]
  17. Younghusband H. B., Bellett A. J. D. 1972; Denaturation pattern of the deoxyribonucleic acid from chicken embryo lethal orphan virus. Journal of Virology 20:855–857
    [Google Scholar]
  18. Zsák L., Bartha A. 1979; Isolation of an adenovirus associated with egg drop syndrome (EDS) in laying hens in Hungary. Magyar Á llatorvosok Lapja 30:691–693
    [Google Scholar]
  19. Zsák L., Kisary J. 1981; Some biological and physicochemical properties of egg drop syndrome (EDS) avian adenovirus strain B8/78.. Archives of Virology (in press)
    [Google Scholar]
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