@article{mbs:/content/journal/jgv/10.1099/0022-1317-56-1-59, author = "Toriyama, S. and Peters, D.", title = "Differentiation between Broccoli Necrotic Yellows Virus and Lettuce Necrotic Yellows Virus by their Transcriptase Activities", journal= "Journal of General Virology", year = "1981", volume = "56", number = "1", pages = "59-66", doi = "https://doi.org/10.1099/0022-1317-56-1-59", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-56-1-59", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "broccoli and lettuce necrotic yellows virus", keywords = "transcriptase activity", abstract = "SUMMARY An endogenous RNA-dependent RNA polymerase has been demonstrated in broccoli necrotic yellows virus (BNYV) particles. The enzyme requires a temperature of 26 °C, a pH value of 7.5, 0.5% NP40 and 4 mm-Mg2+ for maximum synthesis of RNA; Mn2+ inhibited the activity. Addition of EDTA to the reaction mixture did not stimulate the activity in the presence of higher amounts of Mg2+. The in vitro transcription products of BNYV hybridized to the virus genome, but not to that of lettuce necrotic yellows virus (LNYV). The transcriptase activity was abolished by centrifugation on a glycerol gradient after treating the virus with NP40 in a high salt solution; combining the pellet and the upper part of the gradient restored the activity. Cross-complementation of BNYV and LNYV transcription could not be obtained by heterologous combination of the pellet and supernatant fractions of the two viruses.", }