1887

Abstract

SUMMARY

The 79000 mol. wt. measles virion membrane glycoprotein G has been isolated from purified measles virus. Ultracentrifugation of 2% Triton X-100-treated measles virus produced a soluble supernatant fraction containing both G and F, the other external viral membrane protein. Lentil lectin-Sepharose and Sephacryl S-300 column chromatography of this fraction gave a pure preparation of G protein. Sucrose density-gradient centrifugation and SDS-polyacrylamide gel electrophoresis revealed that G was isolated from the virion membrane in the form of a disulphide-linked dimer. Antiserum prepared against purified G reacted only with the G polypeptide of measles virus in a slab gel antibody overlay technique. The antiserum also exhibited haemagglutination inhibition, virus neutralization and haemolysis inhibition activities.

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/content/journal/jgv/10.1099/0022-1317-56-1-185
1981-09-01
2022-08-13
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References

  1. Adams J. M., Imagawa D. T. 1962; Measles antibodies in multiple sclerosis. Proceedings of the Society for Experimental Biology and Medicine 111:562–566
    [Google Scholar]
  2. Anttonen O., Jokinen M., Salmi A., Vainionpaa R., Gahmberg C. G. 1980; The glycoprotein of measles virus. External radioactive labelling of its carbohydrate and partial characterization of the glycopeptide. Biochemical Journal 185:189–194
    [Google Scholar]
  3. Bellini W. J., Trudgett A., McFarlin D. E. 1979; Purification of measles virus with preservation of antigenicity. Journal of General Virology 43:633–639
    [Google Scholar]
  4. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  5. Connolly J. H., Allen I. V., Hurwitz L. J., Miller J. H. D. 1967; Measles virus antibody and antigen in subacute sclerosing panencephalitis. Lancet 1:542–544
    [Google Scholar]
  6. Hall W. W., Martin S. J. 1973; Purification and characterization of measles virus. Journal of General Virology 19:175–188
    [Google Scholar]
  7. Hardwick J. M., Bussell R. H. 1978; Glycoproteins of measles virus under reducing and nonreducing conditions. Journal of Virology 25:687–692
    [Google Scholar]
  8. Horta-Barbosa L., Fuccillo D. A., Hamilton R., Traub R., Ley A., Sever J. L. 1969; Some characteristics of SSPE measles virus. Proceedings of the Society for Experimental Biology and Medicine 134:17–21
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  10. McFarlin D. E., Bellini W. J., Mingioli E. S., Behar T. N., Trudgett A. 1980; Monospecific antibody to the haemagglutinin of measles virus. Journal of General Virology 48:425–429
    [Google Scholar]
  11. McSharry J. J., Compans R. W., Lackland H., Choppin P. W. 1975; Isolation and characterization of the nonglycosylated membrane protein and a nucleocapsid complex from the paramyxovirus SV5. Virology 67:365–374
    [Google Scholar]
  12. Markwell M. A. K., Fox C. F. 1980; Protein-protein interactions within paramyxoviruses identified by native disulfide bonding or reversible chemical cross-linking. Journal of Virology 33:152–166
    [Google Scholar]
  13. Martin R. G., Ames G. N. 1961; A method for determining the sedimentation behavior of enzymes: application to protein mixtures. Journal of Biological Chemistry 236:1372–1379
    [Google Scholar]
  14. Montelaro R. C., Rueckert R. R. 1975; Radiolabeling of proteins and viruses in vitro by acetylation with radioactive acetic anhydride. Journal of Biological Chemistry 250:1413–1421
    [Google Scholar]
  15. Mountcastle W. E., Choppin P. W. 1977; A comparison of the polypeptides of four measles virus strains. Virology 78:463–474
    [Google Scholar]
  16. Norrby E., Gollmar Y. 1972; Appearance and persistence of antibodies against different virus components after regular measles infection. Infection and Immunity 6:240–247
    [Google Scholar]
  17. Norrby E., Gollmar Y. 1975; Identification of measles virus-specific hemolysis-inhibiting antibodies separate from hemagglutination-inhibiting antibodies. Infection and Immunity 11:231–239
    [Google Scholar]
  18. Rustigian R. 1966; Persistent infections of cells in culture by measles virus. I. Development and characteristics of HeLa sublines persistently infected with complete virus. Journal of Bacteriology 92:1792–1804
    [Google Scholar]
  19. Scheid A., Choppin P. W. 1973; Isolation and purification of the envelope proteins of Newcastle disease virus. Journal of Virology 11:263–271
    [Google Scholar]
  20. Scheid A., Choppin P. W. 1977; Two disulfide-linked polypeptide chains constitute the active F protein of paramyxoviruses. Virology 80:54–66
    [Google Scholar]
  21. Scheid A., Caliguiri L. A., Compans R. W., Choppin P. W. 1972; Isolation of paramyxovirus glycoproteins. Association of both hemagglutinating and neuraminidase activities with the larger SV5 glycoprotein. Virology 50:640–652
    [Google Scholar]
  22. Spence L. 1968; Kaolin treatment of sera for removal of nonspecific inhibitors to Asian strains of influenza virus. Proceedings of the Society for Experimental Biology and Medicine 103:425–428
    [Google Scholar]
  23. Tyrrell D. L. J., Norrby E. 1978; Structural polypeptides of measles virus. Journal of General Virology 39:219–229
    [Google Scholar]
  24. Vainionpaa R., Ziola B., Salmi A. 1978; Measles virus polypeptides in purified virions and in infected cells. ActaPathologica et Microbiologica Scandinavica 86:379–385
    [Google Scholar]
  25. Wang C. S., Smith R. L. 1975; Lowry determination of protein in the presence of Triton X-100. Analytical Biochemistry 63:414–417
    [Google Scholar]
  26. Waters D. J., Bussell R. H. 1973; Polypeptide composition of measles and canine distemper viruses. Virology 55:554–557
    [Google Scholar]
  27. Zhdanov V. M. 1980; The measles virus. Molecular and Cellular Biochemistry 29:59–66
    [Google Scholar]
  28. Ziola B., Meurman O., Matikainen M., Salmi A., Kalliomaki J. L. 1978; Determination of human immunoglobulin M rheumatoid factor by a solid-phase radioimmunoassay which uses human immunoglobulin G in antigen-antibody complexes. Journal of Clinical Microbiology 8:131–141
    [Google Scholar]
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