1887

Abstract

SUMMARY

Nuclear and cytoplasmic RNAs, synthesized in cells productively infected with human cytomegalovirus (HCMV) were analysed at various times after infection by liquid and filter DNA-RNA hybridization. Results of these experiments have revealed that: (i) the fraction of the genome transcribed increased as infection progressed. In the nucleus, transcripts represented approx. 20% of the virus DNA sequences at both 2 and 4 h post-infection (p.i.) and 36% of the virus DNA at 40 h p.i.; (ii) the increase in virus sequences among nuclear transcripts at late times was prevented by the DNA synthesis inhibitor, 2′-deoxyfluorouridine; (iii) early virus RNA transcripts were a subset of those represented in late RNA; (iv) two classes of early RNA were identified by competition hybridization; (v) approx. 10% of the late nuclear transcripts were symmetrical. Results of filter hybridization at DNA excess indicated that virus-specific RNA represented 0.6% of RNA labelled from 0 to 2 h p.i., and 1.8% of RNA labelled from 28 to 30 h. Polyadenylated RNA isolated from cytoplasm represented 1.2% and 10% of labelled mRNA at 2 h and 30 h respectively. Our data show that during productive infection of human cells by HCMV, gene expression is under temporal, quantitative and post-transcriptional control.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-56-1-1
1981-09-01
2024-12-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/56/1/JV0560010001.html?itemId=/content/journal/jgv/10.1099/0022-1317-56-1-1&mimeType=html&fmt=ahah

References

  1. Aloni Y., Winocour E., Sachs L. 1968; Characterization of the simian virus 40-specific RNA in virus-yielding and transformed cells. Journal of Molecular Biology 31:415–429
    [Google Scholar]
  2. Aurelian L., Roizman B. 1965; Abortive infection of canine cells by herpes simplex virus. II. The alternative suppression of synthesis of interferon and viral constituents. Journal of Molecular Biology 11:539–548
    [Google Scholar]
  3. Carter T. H., Blanton R. A. 1978; Possible role of the 72000 dalton DNA-binding protein in regulation of adenovirus type 5 early gene expression. Journal of Virology 25:664–674
    [Google Scholar]
  4. Frenkel N., Roizman B. 1972; Ribonucleic acid synthesis in cells infected with herpes simplex virus: controls of transcription and of RNA abundance. Proceedings of the National Academy of Sciences of the United States of America 69:2654–2658
    [Google Scholar]
  5. Furukawa T., Fioretti A., Plotkin S. 1973; Growth characteristics of cytomegalovirus in human fibroblasts with demonstration of protein synthesis early in viral replication. Journal of Virology 11:991–997
    [Google Scholar]
  6. Geelen J. L. M. C., Walig C., Wertheimn P., Van Der Noordaa J. 1978; Human cytomegalovirus DNA. I.Molecular Weight and Infectivity. Journal of Virology 26:813–816
    [Google Scholar]
  7. Huang E.-S., Chen S.-T., Pagano J. S. 1973; Human cytomegalovirus. I. Purification and characterization of viral DNA. Journal of Virology 12:1473–1481
    [Google Scholar]
  8. Rates J. R., Mcauslan B. R. 1967; Messenger RNA synthesis by a ‘coated’ viral genome. Proceedings of the National Academy of Sciences of the United States of America 57:314–320
    [Google Scholar]
  9. Kit S., Dubbs D. R. 1962; Biochemistry of vaccinia-infected mouse-fibroblasts (strain L-M). I. Effects on nucleic acid and protein synthesis. Virology 18:274–285
    [Google Scholar]
  10. Kozak M., Roizman B. 1975; RNA synthesis in cells infected with herpes simplex virus. IX. Evidence for accumulation of abundant symmetric transcripts in nuclei. Journal of Virology 15:36–40
    [Google Scholar]
  11. Lucas J. J., Ginsberg M. S. 1971; Synthesis of virus-specific ribonucleic acid in KB cells infected with type 2 adenovirus. Journal of Virology 8:203–214
    [Google Scholar]
  12. Misra V., Muller M. T., Chantler J. K., Hudson J. B. 1978; Regulation of murine cytomegalovirus gene expression. I. Transcription during productive infection. Journal of Virology 27:263–268
    [Google Scholar]
  13. Parsons J. T., Green M. 1971; Biochemical studies on adenovirus multiplication. XVIII. Resolution of early virus-specific RNA species in adeno 2-infected and -transformed cells. Virology 45:154–162
    [Google Scholar]
  14. Reed I. S., Stark G. R., Alwin J. C. 1976; Autoregulation of simian virus 40 gene A by T antigen. Proceedings of the National Academy of Sciences of the United States of America 73:3083–3087
    [Google Scholar]
  15. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labeling deoxyribonucleic acid to high specificity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  16. Roizman B., Borman G. S., Kamali-Rousta M. 1965; Macromolecular synthesis in cells infected with herpes simplex virus. Nature, London 206:1374–1375
    [Google Scholar]
  17. St. Jeor S., Hutt R. 1977; Cell DNA replication as a function in the synthesis of human cytomegalovirus. Journal of General Virology 37:65–73
    [Google Scholar]
  18. Salzman N. P., Sebring E. D. 1967; Sequential formation of vaccinia virus proteins and viral deoxyribonucleic acid replication. Journal of Virology 1:16–23
    [Google Scholar]
  19. Shatkin A. J. 1963a; Actinomycin D and vaccinia virus infection in HeLa cells. Nature, London 199:357–358
    [Google Scholar]
  20. Shatkin A. J. 1963b; The formation of vaccinia virus protein in the presence of 5-fluorodeoxyuridine. Virology 20:292–301
    [Google Scholar]
  21. Stinsky M. F. 1977; Synthesis of proteins and glycoproteins in cells infected with human cytomegalovirus. Journal of Virology 23:751–767
    [Google Scholar]
  22. Stinsky M. F. 1978; Sequence of protein synthesis in cells infected by human cytomegalovirus: early and late virus-induced polypeptides. Journal of Virology 26:686–701
    [Google Scholar]
  23. Stringer J. R., Holland L. E., Swanstrom R. E., Pivo K., Wagner E. K. 1977; Quantitation of Herpes Simplex Virus Type 1 RNA in infected HeLa cells. Journal of Virology 21:899–901
    [Google Scholar]
  24. Tanaka S., Furukawa T., Plotkin S. 1975; Human cytomegalovirus stimulates host cell RNA synthesis. Journal of Virology 15:297–304
    [Google Scholar]
  25. Thomas D., Green M. 1969; Biochemical studies on adenovirus multiplication. XV. Transcription of the adenovirus type 2 genome during productive infection. Virology 39:205–210
    [Google Scholar]
  26. Vogt V. M. 1973; Purification and further properties of single-stranded-specific nuclease from Aspergillus oryzae.. European Journal of Biochemistry 33:192–200
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-56-1-1
Loading
/content/journal/jgv/10.1099/0022-1317-56-1-1
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error