1887

Abstract

SUMMARY

Hamster kidney cells transformed by BK virus (HKBK cells) were studied at low passage (about 30 subcultures) after transformation and at high passage (about 130 subcultures) after transforming. Several properties (rescue of virus, presence of T antigen, colony formation on plastic, in soft agar, on monolayers of normal cells, and serum dependency of growth) and tumourigenicity of these cells for newborn and adult hamsters were compared. HKBK cells at low passage showed high levels of T antigen, the growth properties of normal cells and low tumour-producing ability, while HKBK cells at high passage showed low levels of T antigen, the growth properties of transformed cells and high tumour-producing ability.

These results may indicate that cellular transformation by BK virus is initiated, but not maintained, by the expression of genes regulating T antigen, and that the maintenance of the transformed state is due to complicated cellular and viral gene interactions occurring during the course of cell life.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-55-2-481
1981-08-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/55/2/JV0550020481.html?itemId=/content/journal/jgv/10.1099/0022-1317-55-2-481&mimeType=html&fmt=ahah

References

  1. Barbanti-brodano G., Possati L., La Placa M. 1971; Inactivation of polykaryocytogenic and hemolytic activites of Sendai virus by phospholipase B (lysolecithinase). Journal of Virology 8:796–800
    [Google Scholar]
  2. Corallini A., Barbanti-brodano G., Bortoloni W., Nenci I., Cassai E., Tampieri M., Portolani M., Borgatti M. 1977; High incidence of ependymomas induced by BK virus. Journal of the National Cancer Institute 59:1561–1564
    [Google Scholar]
  3. Corallini A., Altavilla G., Cecchetti M. G., Fabris G., Grossi M. P., Balboni P. G., Lanza G., Barbanti-brodano G. 1978; Ependymomas, malignant tumours of pancreatic islets, and osteosarcomas induced in hamsters by BK virus, a human papovavirus. Journal of the National Cancer Institute 61:875–880
    [Google Scholar]
  4. Gardner S. D., Field A. M., Coleman D. V., Hulme B. 1971; New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet 1:1253–1257
    [Google Scholar]
  5. Major E. O., Di mayorca G. 1973; Malignant transformation of BHK-21 clone 13 cells by BK virus, a human papovavirus. Proceedings of the National Academy of Sciences of the United States of America 70:3210–3212
    [Google Scholar]
  6. Nase I. M., Karkkainen M., Mantyjarvi R. A. 1975; Transplantable hamster tumors induced with the BK virus. Acta Pathologica et Microbiologica Scandinavica B 83:347–352
    [Google Scholar]
  7. Portolani M., Marzocchi A., Barbanti-brodano G., La placa M. 1974; Prevalence in Italy of antibodies to a new human papovavirus (BK virus). Journal of Medical Microbiology 7:543–546
    [Google Scholar]
  8. Portolani M., Barbanti-brodano G., La placa M. 1975; Malignant transformation of hamster kidney cells by BK virus. Journal of Virology 15:420–422
    [Google Scholar]
  9. Portolani M., Borgatti M., Corallini A., Cassai E., Grossi M. P., Barbanti Brodano G., Possati L. 1978; Stable transformation of mouse, rabbit and monkey cells and abortive transformation of human cells by BK virus, a human papovavirus. Journal of General Virology 38:369–374
    [Google Scholar]
  10. Purchio A. F., Fareed G. C. 1979; Transformation of human embryonic kidney cells by human papovavirus BK. Journal of Virology 29:763–769
    [Google Scholar]
  11. Risser R., Pollack R. 1974; A nonselective analysis of SV40 transformation of mouse 3T3 cells. Virology 59:477–489
    [Google Scholar]
  12. Seehafer J., Salmi A., Colter J. S. 1977; Isolation and characterization of BK virus-transformed hamster cells. Virology 77:356–366
    [Google Scholar]
  13. Seehafer J., Downer D. N., Salmi A., Colter J. S. 1979; Isolation and characterization of BK virus-transformed rat and mouse cells. Journal of General Virology 42:567–578
    [Google Scholar]
  14. Shah K. V., Daniel R. W., Strandberg J. D. 1975; Sarcoma in a hamster inoculated with BK virus, a human papovavirus. Journal of the National Cancer Institute 54:945–950
    [Google Scholar]
  15. Takemoto K. K., Martin M. A. 1976; Transformation of hamster kidney cells by BK papovavirus DNA. Journal of Virology 17:247–253
    [Google Scholar]
  16. Tanaka R., Koprowski H., Iwasaki Y. 1976; Malignant transformation of hamster brain cells in vitro by human papovavirus BK. Journal of the National Cancer Institute 56:671–673
    [Google Scholar]
  17. Van Der noordaa J. 1976; Infectivity, oncogenicity and transforming ability of BK virus and BK virus DNA. Journal of General Virology 30:371–373
    [Google Scholar]
  18. Van Der noordaa J., De jong W., Pauw W., Sol C. J. A., van Strien A. 1979; Transformation and T antigen induction by linearized BK virus DNA. Journal of General Virology 44:843–847
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-55-2-481
Loading
/content/journal/jgv/10.1099/0022-1317-55-2-481
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error