1887

Abstract

Summary

Hamster kidney cells transformed by BK virus (HKBK cells) were studied at low passage (about 30 subcultures) after transformation and at high passage (about 130 subcultures) after transforming. Several properties (rescue of virus, presence of T antigen, colony formation on plastic, in soft agar, on monolayers of normal cells, and serum dependency of growth) and tumourigenicity of these cells for newborn and adult hamsters were compared. HKBK cells at low passage showed high levels of T antigen, the growth properties of normal cells and low tumour-producing ability, while HKBK cells at high passage showed low levels of T antigen, the growth properties of transformed cells and high tumour-producing ability.

These results may indicate that cellular transformation by BK virus is initiated, but not maintained, by the expression of genes regulating T antigen, and that the maintenance of the transformed state is due to complicated cellular and viral gene interactions occurring during the course of cell life.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-55-2-481
1981-08-01
2019-10-18
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-55-2-481
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error