@article{mbs:/content/journal/jgv/10.1099/0022-1317-55-1-95, author = "Sugita, Kenji", title = "Interference between Virulent and Avirulent Strains of Sendai Virus", journal= "Journal of General Virology", year = "1981", volume = "55", number = "1", pages = "95-107", doi = "https://doi.org/10.1099/0022-1317-55-1-95", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-55-1-95", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "virulent", keywords = "avirulent", keywords = "interference", keywords = "Sendai virus", abstract = "Summary Interference between a virulent and an avirulent strain of Sendai virus was studied by plaque morphology and analysis of viral protein and RNA. On simultaneous infection a virulent, large plaque-forming strain (RL) was inhibited by an avirulent, small plaque-forming strain (RS). This interference was dose-dependent and decreased with u.v. irradiation of RS. One infectious particle was sufficient to induce the interference. SDS-polyacrylamide gel electrophoresis showed that interference was detectable in the synthesis of viral P protein; this was abolished when RS was u.v.-irradiated. Both growth and P protein synthesis of RL was restricted by superinfection with RS when this was done within 4 h after infection of RL, but the interference decreased gradually after this period and was not detectable after 8 h. Cycloheximide prolonged the period susceptible to superinfection by RS.", }