1887

Abstract

Summary

Indirect ELISA on plates not precoated with antibodies enabled the detection of cross-reactions among a wider range of serologically related viruses in the tymo-, tombus-, como-, tobamo-, potex-, carla- and potyvirus groups than was possible with the direct double-antibody-sandwich method. Used with purified virus preparations and unfractionated antisera which have to be preabsorbed with crude plant sap the method seems promising for the detection of serological relationships among plant viruses, especially when the latter can be purified only with a low yield or when antisera are in short supply and immuno-electron microscopy is not possible. The quantitative outcome of the test was influenced by the concentration of the reactants, the purity and specific adsorption characteristics of the virus and the length of the immunization period. Strains of Andean potato latent virus (APLV) were detected reliably in crude plant sap when indirect ELISA was done on plates precoated with antiviral antibodies from rabbit or guinea-pig (‘first antibodies’). The trapped virus particles were reacted with antiviral antibodies from chicken (‘second antibodies’) which were then detected by enzyme-labelled rabbit anti-chicken antibodies. Other combinations of ‘first’ and ‘second’ antibodies resulted in non-specific reactions in the presence of crude plant sap. The specificity of direct ELISA was not sufficiently broadened to enable the routine detection of APLV strains when antisera were used which, due to altered immunization schemes, were expected to have an especially broad cross-reactivity.

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1981-07-01
2021-10-23
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References

  1. Barbara D. J., Clark M. F., Thresh J. M., Casper R. 1978; Rapid detection and serotyping of prunus necrotic ringspot virus in perennial crops by enzyme-linked immunosorbent assay. Annals of Applied Biology 90:395–399
    [Google Scholar]
  2. Bar Joseph M., Salomon R. 1980; Heterologous reactivity of tobacco mosaic virus strains in enzyme-linked immunosorbent assays. Journal of General Virology 47:509–512
    [Google Scholar]
  3. Clark M. F., Adams A. M. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
    [Google Scholar]
  4. Crook N. E., Payne C. C. 1980; Comparison of three methods of ELISA for baculoviruses. Journal of General Virology 46:29–37
    [Google Scholar]
  5. Ghabrial S. A., Shepherd R. J. 1980; A sensitive radioimmunosorbent assay for the detection of plant viruses. Journal of General Virology 48:311–317
    [Google Scholar]
  6. Hollings M., Stone O. M. 1965; Studies of pelargonium leaf curl virus. II. Relationships to tomato bushy stunt and other viruses. Annals of Applied Biology 56:87–98
    [Google Scholar]
  7. Koenig R. 1978; ELISA in the study of homologous and heterologous reactions of plant viruses. Journal of General Virology 40:309–318
    [Google Scholar]
  8. Koenig R., Fribourg C. E., Jones R. A. C. 1979; Symptomatological, serological and electrophoretic diversity of isolates of Andean potato latent virus from different regions in the Andes. Phytopathology 69:748–752
    [Google Scholar]
  9. Lesemann D. E., Bozarth R. F., Koenig R. 1980; The trapping of tymovirus particles on electron microscope grids by adsorption and serological binding. Journal of General Virology 48:257–264
    [Google Scholar]
  10. Makkouk K. M., Koenig R., Lesemann D. E. 1981; Characterization of a tombusvirus isolated from eggplant. Phytopathology (in press)
    [Google Scholar]
  11. Matthews R. E. F. 1957 Plant Virus Serology. Cambridge: Cambridge University Press;
  12. Nicolaieff A., van Regenmortel M. H. V. 1980; Specificity of trapping of plant viruses on antibody-coated electron microscope grids. Annales de Virologie 131E:95–110
    [Google Scholar]
  13. Polson A., Von Wechmar B., vanRegenmortel M. H. V. 1980; Isolation of viral IgY antibodies from yolk of immunized hens. Immunological Communications 9:475–493
    [Google Scholar]
  14. Rochow W. F., Carmichael L. E. 1979; Specificity among barley yellow dwarf viruses in enzyme immunosorbent assays. Virology 95:415–430
    [Google Scholar]
  15. Stein A., Loebenstein G., Koenig R. 1979; Dectection of cucumber mosaic virus and bean yellow mosaic virus in gladiolus by enzyme-linked immunosorbent assay (ELISA). Plant Disease Reporter 63:185–188
    [Google Scholar]
  16. Uyemoto J. K. 1980; Detection of maize chlorotic mottle virus serotypes by enzyme-linked immunosorbent assay. Phytopathology 70:290–292
    [Google Scholar]
  17. van Regenmortel M. H. V., Burckard J. 1980; Detection of a wide spectrum of tobacco mosaic virus strains by indirect enzyme-linked immunosorbent assays (ELISA). Virology 106:327–334
    [Google Scholar]
  18. van Regenmortel M. H. V., Nicolaieff A., Burckard J. 1980; Detection of a wide spectrum of virus strains by indirect ELISA and serological trapping electron microscopy (STREM). Acta Horticulturae 110:107–115
    [Google Scholar]
  19. Voller A., Barlett A., Bidwell D. E., Clark M. F., Adams A. N. 1977; The detection of viruses by enzyme-linked immunosorbent assay (ELISA). Journal of General Virology 33:165–167
    [Google Scholar]
  20. Weidemann H. L., Koenig R. 1979; Untersuchungen fiber neue Isolate des Kartoffel-Y-Virus. Gesunde Pflanzen 31:293–296
    [Google Scholar]
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