1887

Abstract

Summary

Human parainfluenza virus type 1 (HA2 virus) grown either in embryonated hens’ eggs or in M. rhesus monkey kidney cells showed differences in size, biological properties and carbohydrate composition. Egg-grown virus showed a larger size (233 nm versus 167 nm), a higher neuraminidase activity (specific activity and initial and maximum velocity) and a higher haemolytic activity than monkey kidney cell-grown virus. The haemagglutinin titre was identical for the HA2 strain grown in both host systems when tested with human O Rh, guinea-pig and hen red blood cells, but reduced by more than 100-fold when tested with grivet monkey red blood cells. In addition, the carbohydrate content (mainly neutral sugars) was higher in egg-grown virus (9.2%) than in virus grown in MK cells (5.7%), and the amino to neutral sugar ratio was lower (1.2 versus 2.1). The sugars were identified as fucose, mannose, galactose, glucose, glucosamine and galactosamine. The prominent neutral monosaccharide was glucose in egg-grown virus and fucose in MK cell-grown virus. HA2 virus infection of MK cells increased fucose and glucose, and decreased mannose and galactose levels.

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/content/journal/jgv/10.1099/0022-1317-55-1-229
1981-07-01
2022-01-25
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References

  1. Ashwell G. 1957; Colorimetric analysis of sugar. Methods in Enzymology 3:73–105
    [Google Scholar]
  2. Aymard-Henry M., Coleman M. T., Dowdle W. R., Laver W. G., Schlld G. C., Webster R. G. 1973; Influenza virus neuraminidase and neuraminidase inhibition test procedures. Bulletin of the World Health Organization 48:199–202
    [Google Scholar]
  3. Chanock R. M., Parrott R. H., Bell J. A., Rowe W. P., Huebner R. J. 1958; New viruses observed in children with respiratory diseases. Public Health Report 73:193–195
    [Google Scholar]
  4. Choppin P. W., Compans R. W. 1975; Reproduction of paramyxoviruses. In Comprehensive Virology vol 4 pp 95–177 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  5. Gunner S. W., Johns J. K. N., Perry M. B. 1961; The gas liquid partition chromatography of carbohydrate derivatives. I. The separation of glycitol and glycoacetates. Canadian Journal of Chemistry 39:1892–1899
    [Google Scholar]
  6. Han R. G., Puck T. T. 1962; Quantitative growth of isolated mammalian cells. Methods in Enzymology 5:90–119
    [Google Scholar]
  7. Kessler N. 1980 Structure et fonctions du myxovirus parainfluenza type 1 humain, souche HA 2. Comparaison avec différents paramyxovirus These Doctorat d’Etat de Sciences, Université Claude-Bernard, Lyon, France:
    [Google Scholar]
  8. Kessler N., Aymard M. 1979; Study of a new strain of paramyxoviruses isolated from wild ducks: structural polypeptides. Journal of General Virology 43:411–415
    [Google Scholar]
  9. Kessler N., Bardeletti G., Aymard M. 1975; The polypeptide composition of human parainfluenza type 1 (strain HA2) and type 3. Comparison with Sendai. Third International Congress of Virology, Madrid, Abstract 334: p 256
    [Google Scholar]
  10. Kessler N., Bardeletti G., Aymard M. 1977; The neuraminidase of human parainfluenza 1 virus (HA2 strain). Journal of General Virology 37:547–556
    [Google Scholar]
  11. Kessler N., Aymard M., Calvet A. 1979; Study of a new strain of paramyxoviruses isolated from wild ducks: antigenic and biological properties. Journal of General Virology 43:273–282
    [Google Scholar]
  12. Kingsbury D. W., Bratt M. A., Choppin P. W., Hanson R. P., Hosaka Y., Ter Meulen V., Norrby E., Plowright W., Rott R., Wunner W. H. 1978; Paramyxoviridae. Intervirology 10:137–152
    [Google Scholar]
  13. Klenk H. D., Choppin P. W. 1969; Chemical composition of the parainfluenza virus SV5. Virology 37:155–157
    [Google Scholar]
  14. Klenk H. D., Choppin P. W. 1970; Glycosphingolipids of plasma membranes of cultured cells and enveloped virus (SV5) grown in these cells. Proceedings of the National Academy of Sciences of the United States of America 66:57–64
    [Google Scholar]
  15. Klenk H. D., Caliguiri L. A., Choppin P. W. 1970; The proteins of the parainfluenza virus SV5. II. The carbohydrate content and glycoproteins of the virion. Virology 42:473–481
    [Google Scholar]
  16. Kohama T., Shimizu K., Ishida N. 1978; Carbohydrate composition of the envelope glycoproteins of Sendai virus. Virology 90:226–234
    [Google Scholar]
  17. Matthews R. E. F. 1979; The classification and nomenclature of viruses. Intervirology 11:133–135
    [Google Scholar]
  18. Nakamura K., Compans R. C. 1979; Host cell and virus strain dependent-differences in oligosaccharides of hemagglutinin glycoproteins of influenza A viruses. Virology 95:8–23
    [Google Scholar]
  19. Reissig J. L., Strominger J. L., Leloir L. F. 1955; A modified colorimetric method for estimation of N-acetylaminosugars. Journal of Biological Chemistry 217:959–966
    [Google Scholar]
  20. Scheid A., Choppin P. W. 1974; Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis and activity by proteolytic cleavage of an inactive precursor protein of Sendaї virus. Virology 57:475–490
    [Google Scholar]
  21. Scheid A., Caliguiri L. A., Compans R. W., Choppin P. W. 1972; Isolation of paramyxovirus glycoproteins. Association of both haemagglutinating and neuraminidase activities with the larger SV5 glycoprotein. Virology 50:640–652
    [Google Scholar]
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