1887

Abstract

Summary

The HSV-1/HSV-2 intertypic recombinant Bx1 (28-1) is heterotypic for the repeat sequences flanking the long unique region of the genome (IR and TR) and expresses both the immediate-early polypeptide IE 110 of HSV-1 and its functionally equivalent polypeptide IE 118 of HSV-2. The genome structures of five subclones of this recombinant and the immediate-early polypeptides they induce have been analysed. Subclone 14 lacked most of the IR sequence, including the region from which part of the mRNA for IE 110 is transcribed, and expressed only the HSV-2 IE 118. Subclone 22 lacked almost all of TR including the gene for IE 118 and induced only the HSV-1 IE 110. Since both subclones produced viable progeny in HFL cells it follows that expression of only one copy of the equivalent genes in TR and IR, here coding for the distinguishable polypeptides IE 110 or IE 118, is sufficient for successive complete cycles of virus replication.

Keyword(s): deletions , HSV and repeated genes
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/content/journal/jgv/10.1099/0022-1317-55-1-179
1981-07-01
2022-01-23
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References

  1. Anderson K. P., Costa R. H., Holland L. E., Wagner E. K. 1980; Characterization of herpes simplex virus type 1 RNA present in the absence of de novo protein synthesis. Journal of Virology 34:9–27
    [Google Scholar]
  2. Brown S. M., Ritchie D. A., Subak-Sharpe J. H. 1973; Genetic studies with herpes simplex type 1. The isolation of temperature sensitive mutants, their arrangement into complementation groups and recombination analysis leading to a linkage map. Journal of General Virology 18:329–346
    [Google Scholar]
  3. Chartrand P., Stow N. D., Timbury M. C., Wilkie N. M. 1979; Physical mapping of paar mutations of herpes simplex virus type 1 and type 2 by intertypic marker rescue. Journal of Virology 31:265–276
    [Google Scholar]
  4. Chartrand P., Crumpacker C. S., Schaffer P. A., Wilkie N. M. 1980; Physical and genetic analysis of the herpes simplex virus DNA polymerase locus. Virology 103:311–326
    [Google Scholar]
  5. Clements J. B., Cortini R., Wilkie N. M. 1976; Analysis of herpesvirus DNA substructure by means of restriction endonucleases. Journal of General Virology 30:243–256
    [Google Scholar]
  6. Cortini R., Wilkie N. M. 1978; Physical maps for HSV type 2 DNA with five restriction endonucleases. Journal of General Virology 39:259–280
    [Google Scholar]
  7. Delius H., Clements J. B. 1976; A partial denaturation map of herpes simplex virus type 1 DNA: evidence for inversions of the unique regions. Journal of General Virology 33:125–133
    [Google Scholar]
  8. Easton A. J., Clements J. B. 1980; Temporal regulation of herpes simplex virus type 2 transcription and characterization of virus immediate-early mRNAs. Nucleic Acids Research 8:2627–2645
    [Google Scholar]
  9. Fenwick M., Walker M., Marshall L. 1980; Some characteristics of an early protein (ICP 22) synthesized in cells infected with herpes simplex virus. Journal of General Virology 47:333–342
    [Google Scholar]
  10. Grafstrom R. H., Alwine J. C., Steinhart W. L., Hill C. W. 1974; Terminal repetitions in herpes simplex virus type 1 DNA. Cold Spring Harbor Symposia on Quantitative Biology 39:679–681
    [Google Scholar]
  11. Grafstrom R. H., Alwine J. C., Steinhart W. L., Hill C. W. 1975; The terminal repetition of herpes simplex virus DNA. Virology 67:144–157
    [Google Scholar]
  12. Hayward G. S., Frenkel N., Roizman B. 1975a; Anatomy of herpes simplex virus DNA: strain differences and heterogeneity in the locations of restriction endonuclease cleavage sites. Proceedings of the National Academy of Sciences of the United States of America 72:1768–1772
    [Google Scholar]
  13. Hayward G. S., Jacob R. J., Wadsworth S. C., Roizman B. 1975b; Anatomy of herpes simplex virus DNA: evidence for four populations that differ in the relative orientations of their long and short components. Proceedings of the National Academy of Sciences of the United States of America 72:4243–4247
    [Google Scholar]
  14. Honess R. W., Roizman B. 1974; Regulation of herpesvirus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins. Journal of Virology 14:8–19
    [Google Scholar]
  15. Lonsdale D. M., Brown S. M., Subak-Sharpe J. H., Warren K. G., Koprowski H. 1979; The polypeptides and the DNA restriction enzyme profiles of spontaneous isolates of herpes simplex virus type 1 from explants of human trigeminal, superior cervical and vagus ganglia. Journal of General Virology 43:151–171
    [Google Scholar]
  16. Macpherson I., Stoker M. 1962; Polyoma transformation of hamster cell clones. An investigation of genetic factors affecting cell competence. Virology 16:147–151
    [Google Scholar]
  17. Maniatis T., Jeffrey A., Van De Sande H. 1975; Chain length determination of small double and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry 14:3787–3794
    [Google Scholar]
  18. Marsden H. S., Crombie I. K., Subak-Sharpe J. H. 1976; Control of protein synthesis in herpesvirus-infected cells: analysis of the polypeptides induced by wild-type and sixteen temperature-sensitive mutants of HSV strain 17. Journal of General Virology 31:347–372
    [Google Scholar]
  19. Marsden H. S., Stow N. D., Preston V. G., Timbury M. C., Wilkie N. M. 1978; Physical mapping of herpes simplex virus-induced polypeptides. Journal of Virology 28:624–642
    [Google Scholar]
  20. Morse L. S., Pereira L., Roizman B., Schaffer P. A. 1978; Anatomy of herpes simplex virus (HSV) DNA. X. Mapping of viral genes by analysis of polypeptides and functions specified by HSV-1 × HSV-2 recombinants. Journal of Virology 26:389–410
    [Google Scholar]
  21. Pereira L., Wolff M. H., Fenwick M., Roizman B. 1977; Regulation of herpes virus macromolecular synthesis. V. Properties of two polypeptides made in HSV-1 and HSV-2 infected cells. Virology 77:733–749
    [Google Scholar]
  22. Preston V. G., Davison A. J., Marsden H. S., Timbury M. C., Subak-Sharpe J. H., Wilkie N. M. 1978; Recombinants between herpes simplex virus types 1 and 2: analysis of genome structures and expression of immediate-early polypeptides. Journal of Virology 28:499–512
    [Google Scholar]
  23. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  24. Sheldrick P., Berthelot N. 1974; Inverted repetitions in the chromosome of herpes simplex virus. Cold Spring Harbor Symposia on Quantitative Biology 39:667–678
    [Google Scholar]
  25. Skare J., Summers W. P., Summers W. C. 1975; Structure and function of herpes virus genomes. I. Comparison of five HSV-1 and two HSV-2 strains by cleavage of their DNA with L’coRI restriction endonuclease. Journal of Virology 15:126–732
    [Google Scholar]
  26. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–533
    [Google Scholar]
  27. Timbury M. C. 1971; Temperature sensitive mutants of herpes simplex virus type 2. Journal of General Virology 13:373–376
    [Google Scholar]
  28. Wagner M. J., Summers W. C. 1978; Structure of the joint region and the termini of the DNA of herpes simplex virus type 1. Journal of Virology 27:374–387
    [Google Scholar]
  29. Watson R. J., Clements J. B. 1978; Characterization of transcription deficient temperature sensitive mutants of HSV-1. Virology 91:364–379
    [Google Scholar]
  30. Watson R. J., Preston C. M., Clements J. B. 1979; Separation and characterization of herpes simplex virus type 1 immediate-early mRNA. Journal of Virology 31:42–52
    [Google Scholar]
  31. Wilkie N. M. 1973; The synthesis and substructure of herpesvirus DNA: the distribution of alkali-labile single strand interruptions in HSV-1 DNA. Journal of General Virology 21:453–467
    [Google Scholar]
  32. Wilkie N. M. 1976; Physical maps for herpes simplex virus type 1 DNA for restriction endonucleases Hindlll, Hpa-I and X.bad. Journal of Virology 20:222–233
    [Google Scholar]
  33. Wilkie N. M., Cortini R. 1976; Sequence arrangement in herpes simplex virus type 1 DNA: identification of terminal fragments in restriction endonuclease digests and evidence for inversions in redundant and unique sequences. Journal of Virology 20:211–221
    [Google Scholar]
  34. Wilkie N. M., Davison A., Chartrand P., Stow N. D., Preston V. G., Timbury M. C. 1978; Recombination in herpes simplex virus: mapping of mutations and analysis of intertypic recombinants. Cold Spring Harbor Symposia on Quantitative Biology 43:827–840
    [Google Scholar]
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