Polypeptides of Infectious Bronchitis Virus. I. Polypeptides of the Virion Free

Abstract

Summary

Infectious bronchitis virus (IBV), strain Beaudette, grown in cultured cells contained five structural proteins with apparent mol. wt. of 170000 (p170), 94000 (gp94), 50000 (pp50) 30000 (gp30) and 26000 (p26). Both gp94 and gp30 are glycopeptides since they were labelled with [H]glucosamine. The only phosphorylated polypeptide was pp50, and both it and gp94 were occasionally resolved into two bands. Two other polypeptides with mol. wt. of 28000 (p28) and 14000 (p14) were sometimes associated with the virus. In egg-grown virus two additional proteins were found with mol. wt. of 110000 (p110) and 75000 (gp75). The cell protein, actin, was also found in highly purified IBV virions. Different serotypes of either tissue culture-grown or egg-grown virus showed one of the two distinct polypeptide patterns of IBV described by Nagy & Lomniczi (1979) and Collins & Alexander (1980, ). Strain Beaudette gave a pattern characteristic of the M type, while strain Connecticut gave a pattern characteristic of the C type. The polypeptides present in Connecticut virus were p170, gp98, pp50, gp28 and p26. Thus, the differences between the two patterns involve the mobility of both the large (gp94/gp98) and small (gp30/gp28) glycopeptides.

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1981-07-01
2024-03-29
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References

  1. Alexander D. J., Collins M. S. 1977; The purification and polypeptide composition of avian infectious bronchitis virus. Microbios 18:87–98
    [Google Scholar]
  2. Bingham R. W. 1975; The polypeptide composition of avian infectious bronchitis virus. Archives of Virology 49:207–216
    [Google Scholar]
  3. Bond C. W., Leibowitz J. L., Robb J. A. 1979; Pathogenic murine coronaviruses. II. Characterization of virus-specific proteins of murine coronaviruses JHMV and A59V. Virology 94:371–384
    [Google Scholar]
  4. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulphate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  5. Collins M. S., Alexander D. J. 1980a; Avian infectious bronchitis virus structural polypeptides: effect of different conditions of disruption and comparison of different strains and isolates. Archives of Virology 63:239–251
    [Google Scholar]
  6. Collins M. S., Alexander D. J. 1980b; The polypeptide composition of isolated surface projections of avian infectious bronchitis virus. Journal of General Virology 48:213–217
    [Google Scholar]
  7. Colman A., Morser J. 1979; Export of proteins from oocytes of Xenopus laevis . Cell 17:517–526
    [Google Scholar]
  8. Garwes D. J., Pocock D. H. 1975; The polypeptide structure of transmissible gastroenteritis virus. Journal of General Virology 29:25–34
    [Google Scholar]
  9. Greengard P. 1978; Phosphorylated proteins as physiological effectors. Science 199:146–152
    [Google Scholar]
  10. Hierholzer J. C. 1976; Purification and biophysical properties of human coronavirus 229E. Virology 75:155–165
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  12. Lamb R. A., Choppin P. W. 1977; The synthesis of Sendai virus polypeptides in infected cells. III. Phosphorylation of polypeptides. Virology 81:382–397
    [Google Scholar]
  13. Lamb R. A., Choppin P. W. 1978; Determination by peptide mapping of the unique polypeptides in Sendai virions and infected cells. Virology 84:469–478
    [Google Scholar]
  14. Lanser J. K., Howard C. R. 1980; The polypeptides of infectious bronchitis virus (IBV-41 strain). Journal of General Virology 46:349–361
    [Google Scholar]
  15. Lomniczi B., Kennedy S. I. T. 1977; Genome of infectious bronchitis virus. Journal of Virology 24:99–107
    [Google Scholar]
  16. MacNaughton M. R. 1980; The polypeptide composition of human and mouse coronaviruses. Archives of Virology 63:75–80
    [Google Scholar]
  17. MacNaughton M. R., Madge H. M. 1977; The polypeptide composition of avian infectious bronchitis virus particles. Archives of Virology 55:47–54
    [Google Scholar]
  18. MacNaughton M. R., Madge H. M., Davies H. A., Dourmashkin R. R. 1977; Polypeptides of the surface projections and the ribonucleoprotein of avian infectious bronchitis virus. Journal of Virology 24:821–825
    [Google Scholar]
  19. Marchalonis J. J. 1969; An enzymatic method for the trace iodination of immunoglobulins and other proteins. Biochemical Journal 113:299–305
    [Google Scholar]
  20. Nagy E., Lomniczi B. 1979; Polypeptide patterns of infectious bronchitis virus serotypes fall into two categories. Archives of Virology 61:341–345
    [Google Scholar]
  21. Naito S., Matsumoto S. 1978; Identification of cellular actin within the rabies virus. Virology 91:151–163
    [Google Scholar]
  22. Pocock D. H., Garwes D. J. 1977; The polypeptides of haemagglutinating encephalomyelitis virus and isolated subviral particles. Journal of General Virology 37:487–499
    [Google Scholar]
  23. Robb J. A., Bond C. W. 1979; Coronaviridae. In Comprehensive Virology vol 14 pp 193–247 Edited by FraenkelConrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  24. Sciotto C. G., Aikawa M., Kohn R. R. 1976; The evaluation of AKR leukemia virus purity: the requirement for both velocity and isopycnic centrifugation methods. Analytical Biochemistry 73:308–320
    [Google Scholar]
  25. Sturman L. S. 1977; Characterization of a coronavirus. I. Structural proteins: effect of preparative conditions on the migration of protein in polyacrylamide gels. Virology 77:637–649
    [Google Scholar]
  26. Sturman L. S., Holmes K. V. 1977; Characterization of a coronavirus. II. Glycoproteins of the viral envelope: tryptic peptide analysis. Virology 77:650–660
    [Google Scholar]
  27. Wang E., Wolf B. A., Lamb R. A., Choppin P. W., Goldberg A. R. 1976; The presence of actin in enveloped viruses. In Cell Motility pp 589–599 Edited by Goldman R., Pollard T. Rosenbaums J. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  28. Wege H., Wege H., Nagashima K., Ter Meulen V. 1979; Structural polypeptides of the murine coronavirus JHM. Journal of General Virology 42:37–47
    [Google Scholar]
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