1887

Abstract

SUMMARY

Cell cultures of the alfalfa looper, (Tn-368), codling moth, (Cp-169) and viper spleen cells (viper-VSW) were inoculated with non-occluded nuclear polyhedrosis virus (AcMNPV-NOV). In both Tn-368 and Cp-169 cells an increase in the concentration of virus protein was detected by ELISA from 9 to 20 h after virus inoculation. An average of 2 ng AcMNPV protein was detected in homogenates of larvae from 12 to 14 h post-infection (p.i.). HzSNPV proteins were first detected in larvae 16 h p.i. At this time approx. 60 ng HzSNPV protein was detected in homogenates of the virus-infected larvae. Virus protein was not detected in viper spleen cells, and larvae inoculated with AcMNPV, or in larvae inoculated with HzSNPV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-54-2-443
1981-06-01
2024-11-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/54/2/JV0540020443.html?itemId=/content/journal/jgv/10.1099/0022-1317-54-2-443&mimeType=html&fmt=ahah

References

  1. Avrameas S. 1969; Coupling of enzymes to proteins with glutaraldehyde. Immunochemistry 6:43–52
    [Google Scholar]
  2. Bazin F., Monsarrat J. R., Bonami P., Croizier G., Maynadier G., QuioT J. M., Vago C. 1972; ParticuleS virales de type Baculovirus observaces chez le crabe Carcinus maenas. Revue des travaux. Institute des peches maritimes 38:205–220
    [Google Scholar]
  3. Bell C. D., Orlob G. B. 1977; Serological studies on virions and polyhedron protein of a nuclear polyhedrosis virus of the cabbage looper, Trichoplusia ni. Virology 78:162–172
    [Google Scholar]
  4. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  5. Clark M. F., Adams A. N. 1977; Characterization of the microplate ELISA for the detection of plant viruses. Journal of General Virology 34:475–483
    [Google Scholar]
  6. Couch J. 1974; Free and occluded virus, similar to baculovirus in hepatopancreas of pink shrimp. Nature, London 247:229–239
    [Google Scholar]
  7. Crawford A. M., Faulkner P., Kalmakoff J. 1978; Comparison of solid-phase radio immunoassays for baculoviruses. Applied and Environmental Microbiology 36:18–24
    [Google Scholar]
  8. Crook N. E., Payne C. C. 1980; Comparison of three methods of ELISA for baculoviruses. Journal of General Virology 46:29–37
    [Google Scholar]
  9. Fenner F. 1976; Classification and nomenclature of viruses. Intervirology 7:1–116
    [Google Scholar]
  10. Gardiner G. R., Stockdale H. 1975; Two tissue culture media for production of lepidopteran cells and nuclear polyhedrosis viruses. Journal of Invertebrate Pathology 25:363–370
    [Google Scholar]
  11. Garvey J. S., Cremer N. E., Sussdorf D. H. 1977; Reagents. In Methods in Immunology 3rd edn pp 522 London: W. A. Benjamin;
    [Google Scholar]
  12. Kelly D. C., Edwards M. L., Evans H. F., Robertson J. S. 1978; The use of the enzyme-linked immunosorbent assay to detect a nuclear polyhedrosis virus in Heliothis armigera larvae. Journal of General Virology 40:465–469
    [Google Scholar]
  13. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  14. Longworth J. F., Carey G. P. 1980; The use of an indirect enzyme-linked immunosorbent assay to detect baculovirus in larvae and adults of Oryctes rhinoceros from Tonga. Journal of General Virology 47:431–438
    [Google Scholar]
  15. Martignoni M., Iwai P. J. 1975; A catalog of viral diseases of insects and mites. USDA Forest Service General Technical Report PNW-40 pp 1–25
    [Google Scholar]
  16. Ohba M., Summers M. D., Hoops P., Smith G. E. 1977; Immunoradiometric assay for baculovirus enveloped nucleocapsids and polyhedron. Journal of Invertebrate Pathology 30:362–368
    [Google Scholar]
  17. Studier F. W. 1973; Analysis of bacteriophage T7 early RNAs and proteins on slab gels. Journal of Molecular Biology 79:237–248
    [Google Scholar]
  18. Summers M. D., Smith G. E. 1978; Baculovirus structural polypeptides. Virology 84:390–402
    [Google Scholar]
  19. Summers M. D., Volkman L. E., Hsieh C. H. 1978; Immunoperoxidase detection of baculovirus antigens in insect cells. Journal of General Virology 40:545–557
    [Google Scholar]
  20. Vaitukaitis J., Robbins B., Neischlag E., Ross G. T. 1971; A method for producing specific antisera with small doses of immunogen. Journal of Clinical Endocrinology 33:988–991
    [Google Scholar]
  21. Vlak J. M. 1979; The proteins of nonoccluded Autographa californica nuclear polyhedrosis virus produced in an established cell line of Spodopterafrugiperda. Journal of Invertebrate Pathology 34:110–118
    [Google Scholar]
  22. Voller A., Bidwell D. E., Bartlett A. 1976a; Enzyme immunoassays in diagnostic medicine: theory and practice. Bulletin of the World Health Organization 53:53–56
    [Google Scholar]
  23. Voller A., Bidwell D. E., Bartlett A. 1976b; Microplate immunoassays for the immunodiagnosis of virus infections. In Manual of Clinical Immunology pp 506–512 Edited by Rose N., Friedman H. Washington D. C.: American Society for Microbiology;
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-54-2-443
Loading
/content/journal/jgv/10.1099/0022-1317-54-2-443
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error