1887

Abstract

SUMMARY

In order to understand the mechanism of congenital cytomegalovirus (CMV) infection, we studied the effect of murine cytomegalovirus (MCMV) on murine ectoplacental cone (EPC) cells . Cytopathic effects (c.p.e.) were seen in many MCMV-infected EPC cell cultures 5 to 7 days after exposure to MCMV. The infected cells showed intranuclear inclusions characteristic of CMV infection when stained with May-Grunwald-Giemsa. Culture fluids collected from MCMV-infected EPC cells after 4 or more days of culture caused c.p.e. when co-cultivated with mouse embryo fibroblasts (MEF). Employment of the anti-complement immunofluorescence (ACIF) test detected MCMV-specific antigens, hybridization localized MCMV DNA and electron microscopy detected the presence of the viral particles in the MCMV-infected EPC cells. Thus, exposure of EPC cells to MCMV resulted in a productive infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-54-2-415
1981-06-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/54/2/JV0540020415.html?itemId=/content/journal/jgv/10.1099/0022-1317-54-2-415&mimeType=html&fmt=ahah

References

  1. Baskar J. F. 1975 Tumorigenicity of three cell types derived from a mouse teratocarcinoma cell line D. Sc. thesis Johns Hopkins University, School of Hygiene and Public Health; Baltimore Maryland U.S.A.:
    [Google Scholar]
  2. Benirschke K., Mendoza G. R., Bazeley P. L. 1974; Placental and fetal manifestations of cytomegalovirus infection. Virchows Archiv B. Cell Pathology 16:121–139
    [Google Scholar]
  3. Cochard A. M., Vinh L. T., Lelong M. 1963; The placenta in congenital cytomegaly: anatomical study of three personal cases. Archives Francoises de Pediatrie 20:35–46
    [Google Scholar]
  4. Davis G. L. 1969; Cytomegalovirus in the inner ear. Case report and electron microscopic study. Annals of Otology, Rhinology and Laryngology 78:1179–1188
    [Google Scholar]
  5. Dutko F. J., Oldstone M. B. A. 1979; Murine cytomegalovirus infects spermatogenic cells. Proceedings of the National Academy of Sciences of the United States of America 76:2988–2991
    [Google Scholar]
  6. Eachempati U., Woods R. E. 1976; Cytomegalic virus disease in pregnancy. Obstetrics and Gynecology 47:615–618
    [Google Scholar]
  7. Feldman R. A. 1969; Cytomegalovirus infection during pregnancy. American Journal of Diseases of Children 117:517–522
    [Google Scholar]
  8. Gardner R. L., Papaioannou V. E., Barton S. C. 1973; Origin of the ectoplacental cone and secondary giant cells in mouse blastocysts reconstituted from isolated trophoblast and inner cell mass. Journal of Embryology and Experimental Morphology 30:561–572
    [Google Scholar]
  9. Greene P. J., Betlach M. C., Boyer H. W., Goodman H. M. 1974; The EcoRI restriction endonuclease. In Methods in Molecular Biology pp 87–105 Edited by Wickner R. B. New York: Marcel Dekker;
    [Google Scholar]
  10. Huang E-S., Pagano J. S. 1977; Comparative diagnosis of cytomegalovirus: new approach. Comparative Diagnostic Virology of Diseases 1:241–285
    [Google Scholar]
  11. Johnson K. P. 1969; Mouse cytomegalovirus: placental infection. Journal of Infectious Diseases 120:445–450
    [Google Scholar]
  12. Kilpatrick B. A., Huang E. S., Pagano J. S. 1976; Analysis of cytomegalovirus genomes with restriction endonucleases HinD III and EcoR-1. Journal of Virology 18:1095–1105
    [Google Scholar]
  13. Kirby D. R. S. 1971; The transplantation of mouse eggs and trophoblast to extrauterine sites. In Methods in Mammalian Embryology pp 146–156 Edited by Daniel J. Jr San Francisco: Freeman;
    [Google Scholar]
  14. Kumar M. L., Nankervis G. A. 1979; Cytomegalovirus infections. Southern Medical Journal 72:854–861
    [Google Scholar]
  15. Lang D. J., Kummer J. F. 1972; Demonstration of cytomegalovirus in semen. New England Journal of Medicine 287:756–758
    [Google Scholar]
  16. Luckett W. P. 1978; Origin and differentiation of the yolk sac and extraembryonic mesoderm in presomite human and rhesus monkey embryos. American Journal of Anatomy 152:59–87
    [Google Scholar]
  17. Medearis D. N. Jr 1964; Mouse cytomegalovirus infection. II. Observations during prolonged infections. American Journal of Hygiene 80:103–112
    [Google Scholar]
  18. Mintz B. 1971; Allophenic mice of multi-embryo origin. In Methods in Mammalian Embryology pp 305–319 Edited by Daniel J. Jr San Francisco: Freeman;
    [Google Scholar]
  19. Montplaisir S., Belloncik S., Leduc N. P., Onji P. A., Martineau B., Kurstak E. 1972; Electron microscopy in the rapid diagnosis of cytomegalovirus: ultrastructural observation and comparison of methods of diagnosis. Journal of Infectious Diseases 125:533–538
    [Google Scholar]
  20. Neighbour P. A. 1978; Studies on the susceptibility of the mouse preimplantation embryo to infection with cytomegalovirus. Journal of Reproduction and Fertility 54:15–20
    [Google Scholar]
  21. Neighbour P. A., Fraser L. R. 1978; Murine cytomegalovirus and fertility: potential sexual transmission and the effect of this virus on fertilization in vitro. Fertility and Sterility 30:216–222
    [Google Scholar]
  22. Weller T. H. 1971; The cytomegaloviruses: ubiquitous agents with protean clinical manifestations. New England Journal of Medicine 285:203–214 267–274
    [Google Scholar]
  23. Weller T. H., Hanshaw J. B. 1962; Virologic and clinical observations on cytomegalic inclusion disease. New England Journal of Medicine 226:1233–1244
    [Google Scholar]
  24. Zain B. S., Roberts R. J. 1977; A new specific endonuclease from Xanthomonas badrii. Journal of Molecular Biology 115:249–255
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-54-2-415
Loading
/content/journal/jgv/10.1099/0022-1317-54-2-415
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error