1887

Abstract

SUMMARY

The hybrid plasmid consists of the herpes simplex virus type 1 (HSV-1) HI fragment, which contains the thymidine kinase (TK) gene, inserted into the vector pAT 153. When DNA was microinjected into nuclei of oocytes, functional HSV-1-specific TK was produced, showing that transcription and translation of the gene occurred. Investigation of -specific RNA by ‘Southern’ blot hybridization revealed that all regions of the hybrid plasmid were transcribed by RNA polymerase II, but sequences present in TK mRNA were most highly represented in stable transcripts.

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1981-06-01
2022-01-18
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