1887

Abstract

SUMMARY

The multiplication of the DG strain of cowpea severe mosaic virus (CPsMV-DG) was studied in cowpea protoplasts prepared from infected leaves and in protoplasts inoculated . Up to six proteins were detected in DG-infected cells which were either absent in mock-infected protoplasts or present in smaller amounts. Their mol. wt. were estimated to be 125000, 98000, 86000, 65000, 39000 and 22000. The latter two probably represent the two viral capsid proteins. The 125000 mol. wt. protein was found early in infection and was synthesized at a high rate throughout infection. It was detected, together with the 86000 mol. wt. protein, in protoplasts that had been inoculated with purified bottom component alone.

CPsMV was also translated in a messenger-dependent reticulocyte lysate. Total DG strain RNA directed the synthesis of polypeptides with mol. wt. of 200000, 125000, 108000, 98000 and 42000. No cleavage of the products was observed by varying the temperature or the concentration of dithiothreitol (DTT) in the translation mixture. The polypeptides induced by CPsMV-DG were compared with those induced by the SB strain of cowpea mosaic virus. Since the 170000 and 30000 mol. wt. proteins typical of the SB strain were not detected among the DG-specific proteins either or , a different processing of the large precursor of 200000 mol. wt. seems to take place.

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1981-06-01
2021-10-19
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References

  1. Agrawal H. O. 1964; Identification of cowpea mosaic virus isolates. Mededelingen Landbouwhogeschool 64:1–53
    [Google Scholar]
  2. Aoki S., Takebe I. 1969; Infection of tobacco mesophyll protoplasts by tobacco mosaic virus ribonucleic acid. Virology 39:439–448
    [Google Scholar]
  3. Beier H., Bruening G. 1975; The use of an abrasive in the isolation of cowpea leaf protoplasts which support the multiplication of cowpea mosaic virus. Virology 64:272–276
    [Google Scholar]
  4. Beier H., Bruening G. 1976; Factors influencing the infection of cowpea protoplasts by cowpea mosaic virus RNA. Virology 72:363–369
    [Google Scholar]
  5. Beier H., Siler D. J., Russell M. L., Bruening G. 1977; Survey of susceptibility to cowpea mosaic virus among protoplasts and intact plants from Vigna sinensis lines. Phytopathology 67:917–921
    [Google Scholar]
  6. Beier H., Bruening G., Russell M. L., Tucker C. L. 1979; Replication of cowpea mosaic virus in protoplasts isolated from immune lines of cowpeas. Virology 95:165–175
    [Google Scholar]
  7. Bruening G. 1969; The inheritance of top component formation in cowpea mosaic virus. Virology 37:577–584
    [Google Scholar]
  8. Daubert S. D., Bruening G., Najarian R. C. 1978; Protein bound to the genome RNAs of cowpea mosaic virus. European Journal of Biochemistry 92:45–51
    [Google Scholar]
  9. Davies J. W., Aalbers A. M. J., Stuik E. J., Van Kammen A. 1977; Translation of cowpea mosaic virus RNA in a cell-free extract from wheat germ. FEBS Letters 77:265–269
    [Google Scholar]
  10. De Jager C. P. 1979; Cowpea severe mosaic virus. Commonwealth Mycological Institute/Association of Applied Biologists Descriptions of Plant Viruses no. 209
    [Google Scholar]
  11. El Manna M. M., Bruening G. 1973; Polyadenylate sequences in the ribonucleic acids of cowpea mosaic virus. Virology 56:198–206
    [Google Scholar]
  12. Goldbach R., Rezelman G., Van Kammen A. 1980; Independent replication and expression of B-component RNA of cowpea mosaic virus. Nature, London 286:297–300
    [Google Scholar]
  13. Hibi T., Rezelman G., Van Kammen A. 1975; Infection of cowpea mesophyll protoplasts with cowpea mosaic virus. Virology 64:308–318
    [Google Scholar]
  14. Issinger O. G., Falk H. 1976; Comparative studies on the structural proteins of T3 and T7 phages. Archives of Virology 52:217–231
    [Google Scholar]
  15. Jense H., Knauert F., Ehrenfeld E. 1978; Two initiation sites for translation of poliovirus RNA in vitro. Comparison of LSc and Mahoney Strains. Journal of Virology 28:387–394
    [Google Scholar]
  16. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  17. Laskey R. A., Mills A. D. 1975; Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  18. Pelham H. R. B. 1979; Synthesis and proteolytic processing of cowpea mosaic virus proteins in reticulocyte lysates. Virology 96:463–477
    [Google Scholar]
  19. Pelham H. R. B., Jackson R. J. 1976; An efficient mRNA-dependent translation system from reticulocyte lysates. European Journal of Biochemistry 67:247–256
    [Google Scholar]
  20. Reijnders L., Aalbers A. M. J., Van Kammen A., Thuring R. W. J. 1974; Molecular weights of plant viral RNAs determined by gel electrophoresis under denaturing conditions. Virology 60:515–521
    [Google Scholar]
  21. Rezelman G., Goldbach R., Van Kammen A. 1980; Expression of the bottom component RNA of cowpea mosaic virus in cowpea protoplasts. Journal of Virology (in press)
    [Google Scholar]
  22. Rottier P. J. M. 1980 Viral protein synthesis in cowpea mosaic virus infected protoplasts Ph. D. thesis Agricultural University, Wageningen; The Netherlands:
    [Google Scholar]
  23. Rottier P. J. M., Rezelman G., Van Kammen A. 1979; The inhibition of cowpea mosaic virus replication by actinomycin D. Virology 92:299–309
    [Google Scholar]
  24. Siegel A., Hari V., Kolacz K. 1978; The effect of tobacco mosaic virus infection on host and virus-specific protein synthesis in protoplasts. Virology 85:494–503
    [Google Scholar]
  25. Stanley J., Rottier P., Davies J. W., Zabel P., Van Kammen A. 1978; A protein linked to the 5′-termini of both RNA components of the cowpea mosaic virus genome. Nucleic Acids Research 5:4505–4522
    [Google Scholar]
  26. Steele K. P., Frist R. H. 1978; Characterization of the 3’ termini of the RNAs of cowpea mosaic virus. Journal of Virology 26:243–248
    [Google Scholar]
  27. Swaans H., Van Kammen A. 1973; Reconsideration of the distinction between severe and yellow strains of cowpea mosaic virus. Netherlands Journal of Plant Pathology 19:257–265
    [Google Scholar]
  28. Toongmeearkom P., Goodman R. M. 1978; Complementation and pseudorecombination between ribonucleic acids from two natural isolates of cowpea mosaic virus (severe subgroup). Virology 85:75–83
    [Google Scholar]
  29. Van Kammen A. 1968; The relationship between the components of CPMV. I. Two nucleoprotein particles necessary for the infectivity of CPMV. Virology 34:312–318
    [Google Scholar]
  30. Wood H. A., Bancroft J. B. 1965; Activation of a plant virus by related incomplete nucleoprotein particles. Virology 27:94–102
    [Google Scholar]
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