RT Journal Article SR Electronic(1) A1 Mamoun, R. Z. A1 Astier, T. A1 Guillemain, B.YR 1981 T1 Establishment and Propagation of a Bovine Leukaemia Virus-producing Cell Line Derived from the Leukocytes of a Leukaemic Cow JF Journal of General Virology, VO 54 IS 2 SP 357 OP 365 DO https://doi.org/10.1099/0022-1317-54-2-357 PB Microbiology Society, SN 1465-2099, AB SUMMARY A cell line (LB59Ly) derived from the leukocytes of a leukaemic cow was established as a monolayer, which spontaneously released large amounts of a retrovirus. This virus was found to be indistinguishable from the bovine leukaemia virus (BLV) produced by the commonly used high-producing heterologous cell line (FLK-BLV). Like the latter, its reverse transcriptase activity was greater in the presence of Mg2+ cations than in the presence of Mn2+ cations; its polyacrylamide gel electrophoresis pattern showed the presence of gp51, p24, p15, p12 and p10, and the antigenicity of the two major proteins completely cross-reacted with those of BLV from FLK-BLV cells. The virus was infectious and induced early and late polykaryocytosis, the specificity of which was demonstrated by use of specific anti-BLV sera., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-54-2-357