RT Journal Article SR Electronic(1) A1 Khittoo, Govindranathsing A1 Weber, Joseph M.YR 1981 T1 The Nature of the DNA Associated with Incomplete Particles of Adenovirus Type 2 JF Journal of General Virology, VO 54 IS 2 SP 343 OP 355 DO https://doi.org/10.1099/0022-1317-54-2-343 PB Microbiology Society, SN 1465-2099, AB SUMMARY The nature of the DNA in incomplete particles (IP) synthesized by adenovirus type 2 and the ts4 mutant which accumulates such particles were analysed by agarose gel electrophoresis, restriction endonuclease cleavage and blot hybridization techniques. IP DNA consisted of a heterogeneous population of subgenomic-size DNA (IPSD1) and smaller molecules ranging from about 1000 base pairs to 200 base pairs (IPSD2). IPSD1 from ts4 was more heterogeneous than that from wild-type (wt), but both contained sequences from all parts of the viral genome. IPSD2 contained heterogeneous cellular sequences and viral sequences from the left 4.4% of the genome. An endonuclease activity associated with IP and virions was capable of digesting viral or cellular DNA to IPSD2-like fragments suggesting a possible origin for these molecules., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-54-2-343