1887

Abstract

SUMMARY

The nature of the DNA in incomplete particles (IP) synthesized by adenovirus type 2 and the 4 mutant which accumulates such particles were analysed by agarose gel electrophoresis, restriction endonuclease cleavage and blot hybridization techniques. IP DNA consisted of a heterogeneous population of subgenomic-size DNA (IPSD) and smaller molecules ranging from about 1000 base pairs to 200 base pairs (IPSD). IPSD from 4 was more heterogeneous than that from wild-type (wt), but both contained sequences from all parts of the viral genome. IPSD contained heterogeneous cellular sequences and viral sequences from the left 4.4% of the genome. An endonuclease activity associated with IP and virions was capable of digesting viral or cellular DNA to IPSD-like fragments suggesting a possible origin for these molecules.

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1981-06-01
2024-04-20
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References

  1. Arrand J. R., Roberts R. J. 1979; The nucleotide sequences at the termini of adenovirus-2 DNA. Journal of Molecular Biology 128:577–594
    [Google Scholar]
  2. Birg F., Favaloro J., Kamen R. 1977; Analysis of polyoma virus nuclear RNA by mini-blot hybridization. Proceedings of the National Academy of Sciences of the United States of America 74:3138–3142
    [Google Scholar]
  3. Botchan M., Topp W., Sambrook J. 1976; The arrangement of simian virus 40 sequences in the DNA of transformed cells. Cell 9:269–287
    [Google Scholar]
  4. Boudin M. L., D’Halluin J. C., Cousin C., Boulanger P. 1980; Human adenovirus type 2 protein Ilia. II. Maturation and encapsidation. Virology 101:144–156
    [Google Scholar]
  5. Burlingham B. T., Doerfler W. 1972; An endonuclease in cells infected with adenovirus and associated with adenovirions. Virology 48:1–13
    [Google Scholar]
  6. Burlingham B. T., Doerfler W., Pettersson U., Philipson L. 1971; Adenovirus endonuclease: association with the penton of adenovirus type 2. Journal of Molecular Biology 60:45–64
    [Google Scholar]
  7. Burlingham B. T., Brown D. T., Doerfler W. 1974; Incomplete particles of adenovirus. I. Characteristics of the DNA associated with incomplete adenovirus of types 2 and 12. Virology 60:419–430
    [Google Scholar]
  8. Casjens S., King J. 1975; Virus Assembly. Annual Review of Biochemistry 44:555–611
    [Google Scholar]
  9. Daniell E. 1976; Genome structure of incomplete particles of adenovirus. Journal of Virology 19:685–708
    [Google Scholar]
  10. Denhardt D. 1966; A membrane filter technique for the detection of complementary DNA. Biochemical and Biophysical Research Communications 23:641–646
    [Google Scholar]
  11. D’halluin J. C., Milleville M., Boulanger P. A., Martin G. R. 1978; Temperature-sensitive mutant of adenovirus type 2 blocked in virion assembly: accumulation of light intermediate particles. Journal of Virology 26:344–356
    [Google Scholar]
  12. Edvardsson B., Everitt E., Jornvall H., Prage L., Philipson L. 1976; Intermediates in adenovirus assembly. Journal of Virology 19:533–547
    [Google Scholar]
  13. Edvardsson B., Ustacelebi S., Williams J., Philipson L. 1978; Assembly intermediates among adenovirus type 5 temperature-sensitive mutants. Journal of Virology 25:641–651
    [Google Scholar]
  14. Everitt E., Meador S. A., Levine S. A. 1977; Synthesis and processing of the precursor to the major core protein of adenovirus type 2. Journal of Virology 21:199–214
    [Google Scholar]
  15. Hammarskjold M. L., Winberg G., Norrby E., Wadell G. 1977; Isolation of incomplete adenovirus 16 particles containing viral and host cell DNA. Virology 82:449–461
    [Google Scholar]
  16. Igarashi K., Niiyama Y., Tsukamoto K., Kurokawa T., Sugino Y. 1975; Biochemical studies on bovine adenovirus type 3. II. Incomplete virus. Journal of Virology 16:634–641
    [Google Scholar]
  17. Ishibashi M., Maizel J. V. Jr 1974; The polypeptides of adenovirus. V. Young virions, structural intermediate between top components and aged virions. Virology 57:409–424
    [Google Scholar]
  18. Khittoo G., Weber J. 1977; Genetic analysis of adenovirus type 2. VI. Adenovirus ts4 gene function is required for DNA encapsidation. Virology 81:126–137
    [Google Scholar]
  19. Mak S. 1971; Defective virions in human adenovirus type 12. Journal of Virology 7:426–433
    [Google Scholar]
  20. Mak I., Ezoe H., Mak S. 1979; Structure and function of adenovirus type 12 defective virions. Journal of Virology 32:240–250
    [Google Scholar]
  21. Maniatis T., Jeffrey A., Kleid D. G. 1975; Nucleotide sequence of the rightward operator of phage lambda. Proceedings of the National Academy of Sciences of the United States of America 72:1184–1188
    [Google Scholar]
  22. Marusyk R. G., Morgan A. R., Wadell G. 1975; Association of endonuclease activity with serotypes belonging to three subgroups of human adenoviruses. Journal of Virology 16:456–458
    [Google Scholar]
  23. Niiyama Y., Igarashi K., Tsukamoto K., Kurokawa T., Sugino Y. 1975; Biochemical studies on bovine adenovirus type 3.1. Purification and properties. Journal of Virology 16:621–623
    [Google Scholar]
  24. Padmanabhan R., Stabel S., Winterhoeff U., Doerfler W. 1979; Specificity and mode of cleavage of the pH 4-0 endonuclease from adenovirus type 2-infected KB cells. Nucleic Acids Research 6:3161–3176
    [Google Scholar]
  25. Plaat D., Weber J. 1979; On the mechanism of arginine requirement for adenovirus synthesis. Archives of Virology 60:187–196
    [Google Scholar]
  26. Prage L. S., Hoglund S., Philipson L. 1972; Structural proteins of adenoviruses. III. Characterization of incomplete particles of adenovirus type 3. Virology 49:745–757
    [Google Scholar]
  27. Rainbow A., Mak S. 1970; Functional heterogeneity of virions in human adenovirus type 2 and 12. Journal of Virology 5:188–193
    [Google Scholar]
  28. Reif U., Winterhoff U., Doerfler W. 1977a; Characterization of the pH 4-0 endonuclease from adenovirus type 2 infected KB cells. European Journal of Biochemistry 73:327–333
    [Google Scholar]
  29. Reif U., Winterhoff U., Lundholm U., Philipson L., Doerfler W. 1977b; Purification of an endonuclease from adenovirus-infected KB cells. European Journal of Biochemistry 73:313–325
    [Google Scholar]
  30. Rosenwirth B., Tjia S., Westphal M., Doerfler W. 1974; Incomplete particles of adenovirus. II. Kinetics of formation and polypeptide composition of adenovirus type 2. Virology 60:431–437
    [Google Scholar]
  31. Schaller J. P., Yohn D. S. 1974; Transformation potentials of the non-infectious (defective) component in pools of adenoviruses type 12 and simian adenovirus 7. Journal of Virology 14:392–401
    [Google Scholar]
  32. Sharp P. A., Sambrook J., Sugden B. 1973; Detection of two restriction endonuclease activities in Haemophilusparainfluenzae using analytical agarose gel electrophoresis. Biochemistry 12:3055–3061
    [Google Scholar]
  33. Southern E. M. 1975; Detection of specific sequence among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–518
    [Google Scholar]
  34. Sundquist B., Everitt E., Philipson L., Hoglund S. 1973; Assembly of adenoviruses. Journal of Virology 11:449–459
    [Google Scholar]
  35. Tibbetts C. 1977; Viral sequences from incomplete particles of human adenovirus type 7. Cell 12:243–249
    [Google Scholar]
  36. Tjia S., Fanning E., Schick J., Doerfler W. 1977; Incomplete particles of adenovirus type 2. III. Viral and cellular DNA sequences in incomplete particles. Virology 76:365–379
    [Google Scholar]
  37. Tyndall T., Younghusband H. B., Bellett A. J. D. 1978; Some adenovirus DNA is associated with the DNA of permissive cells during productive or restricted growth. Journal of Virology 25:1–10
    [Google Scholar]
  38. Van Roy F., Engler G., Fiers W. 1979; Isolation and characterization of a specific deletion mutant of human adenovirus type 2. Virology 96:486–502
    [Google Scholar]
  39. Wadell G., Hammarskjold M. L., Varsanyi T. 1973; Incomplete virus particles of adenovirus type 16. Journal of General Virology 20:287–302
    [Google Scholar]
  40. Weber J. 1976; Genetic analysis of adenovirus type 2. III. Temperature sensitivity of processing of viral proteins. Journal of Virology 17:462–471
    [Google Scholar]
  41. Winberg G., Wadell G. 1977; Structural polypeptides of adenovirus type 16 incomplete particles. Journal of Virology 22:389–401
    [Google Scholar]
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