%0 Journal Article %A Feldman, Larry %A Blankenship, Mayme L. %A Ben-Porat, Tamar %T Isolation and Characterization of a Temperature-sensitive Uncoating Mutant of Pseudorabies Virus %D 1981 %J Journal of General Virology, %V 54 %N 2 %P 333-342 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-54-2-333 %I Microbiology Society, %X SUMMARY During the course of characterizing a series of temperature-sensitive mutants of pseudorabies virus, we found one (designated tsL) that did not produce cytopathic changes in rabbit kidney cells at the non-permissive temperature (41 °C). Although the mutant adsorbed to and penetrated the cells in a normal fashion, virus RNA was not synthesized at 41 °C in the infected cells. However, if the cells were first incubated at the permissive temperature (32 °C), virus RNA synthesis occurred at the non-permissive temperature. This occurred even if, during the incubation period at 32 °C, the expression of viral functions was prevented by treatment with an inhibitor of protein synthesis. The DNA in tsL virions did not appear in the cell nucleus at 41 °C, and full, non-enveloped nucleocapsids could be recovered from the cytoplasm of tsL virus-infected cells. These results show that the nucleocapsids of tsL remain intact at the non-permissive temperature and that tsL is an uncoating mutant. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-54-2-333