1887

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Volume 54, Issue 2

Isolation and Characterization of a Temperature-sensitive Uncoating Mutant of Pseudorabies Virus Free

Abstract

SUMMARY

During the course of characterizing a series of temperature-sensitive mutants of pseudorabies virus, we found one (designated L) that did not produce cytopathic changes in rabbit kidney cells at the non-permissive temperature (41 °C). Although the mutant adsorbed to and penetrated the cells in a normal fashion, virus RNA was not synthesized at 41 °C in the infected cells. However, if the cells were first incubated at the permissive temperature (32 °C), virus RNA synthesis occurred at the non-permissive temperature. This occurred even if, during the incubation period at 32 °C, the expression of viral functions was prevented by treatment with an inhibitor of protein synthesis. The DNA in L virions did not appear in the cell nucleus at 41 °C, and full, non-enveloped nucleocapsids could be recovered from the cytoplasm of L virus-infected cells. These results show that the nucleocapsids of L remain intact at the non-permissive temperature and that L is an uncoating mutant.

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© Journal of General Virology 1981
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1981-06-01
2024-04-18
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Isolation and Characterization of a Temperature-sensitive Uncoating Mutant of Pseudorabies Virus
J. Gen. Virol. 54, 333 (1981); https://doi.org/10.1099/0022-1317-54-2-333
/content/journal/jgv/10.1099/0022-1317-54-2-333
/content/journal/jgv/10.1099/0022-1317-54-2-333
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