RT Journal Article SR Electronic(1) A1 Giraudon, P. A1 Wild, T. F.YR 1981 T1 Monoclonal Antibodies Against Measles Virus JF Journal of General Virology, VO 54 IS 2 SP 325 OP 332 DO https://doi.org/10.1099/0022-1317-54-2-325 PB Microbiology Society, SN 1465-2099, AB SUMMARY Monoclonal antibodies were produced in vitro by fusing mouse myeloma cells (SP2) with spleen cells derived from Balb/c mice immunized with purified measles virus. Fifteen independent hybrid cell lines, isolated from two separate fusions, were maintained in culture for up to 5 months without loss of their antibody-secreting activity. Radioimmunoprecipitation and polyacrylamide gel electrophoresis showed that in five of the hybrid lines the antibodies were directed against haemagglutinin, in two against the nucleoprotein, and in one against L protein. The remaining seven hybridomas did not precipitate viral antigens under the experimental conditions employed even though they gave positive immunofluorescence against measles virus-infected cells. Monoclonal haemagglutinin antibodies displayed anti-haemagglutinating activity and neutralized measles virus infectivity but not canine distemper virus (CDV)., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-54-2-325