1887

Abstract

SUMMARY

Monoclonal antibodies were produced by fusing mouse myeloma cells (SP2) with spleen cells derived from Balb/c mice immunized with purified measles virus. Fifteen independent hybrid cell lines, isolated from two separate fusions, were maintained in culture for up to 5 months without loss of their antibody-secreting activity. Radioimmunoprecipitation and polyacrylamide gel electrophoresis showed that in five of the hybrid lines the antibodies were directed against haemagglutinin, in two against the nucleoprotein, and in one against L protein. The remaining seven hybridomas did not precipitate viral antigens under the experimental conditions employed even though they gave positive immunofluorescence against measles virus-infected cells. Monoclonal haemagglutinin antibodies displayed anti-haemagglutinating activity and neutralized measles virus infectivity but not canine distemper virus (CDV).

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-54-2-325
1981-06-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/54/2/JV0540020325.html?itemId=/content/journal/jgv/10.1099/0022-1317-54-2-325&mimeType=html&fmt=ahah

References

  1. Basle M., Rebel A., Pouplard A., Kouyoumdjian S., Filmon R., Lepatezour A. 1979; Mise en evidence d’antigenes viraux de rougeole dans les osteoclastes de la maladie osseuse de Paget. Comptes Rendus hebdomadaires des seances de I’Academie des Sciences 289:225–228
    [Google Scholar]
  2. Baublis J. V., Payne F. E. 1968; Measles antigen and syncytium in brain cultures from subacute sclerosing panencephalitis (SSPE). Proceedings of the Society for Experimental Biology and Medicine 129:593–597
    [Google Scholar]
  3. Bonner M. W., Laskey R. A. 1974; A film detection method for tritium-labelled protein and nucleic acid in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  4. Bouteille M., Fontaine C., Vedrenne C., Delarue J. 1965; Sur un cas d’encephalite subaigue a inclusions. Etude anatomoclinique et ultrastructurale. Revue Neurologique (Paris) 113:454–458
    [Google Scholar]
  5. Buttin G., Lee Guern G., Phalente L., Lin E. C. C., Medrano L., Cazenave P. A. 1978; Lymphocyte hybridomas. In Current Topics in Microbiology and Immunology vol 81: pp 27–36 Edited by Melchers F., Potter M., Warner N. Berlin, Heidelberg and New York: Springer Verlag;
    [Google Scholar]
  6. Cathala F., Brown P. 1972; The possible viral aetiology of disseminated sclerosis. Journal of Clinical Pathology 25: (suppl.) 141–151
    [Google Scholar]
  7. Connoly J. H., Allen I. V., Hurwitz L. J., Millar J. H. D. 1967; Measles virus antibody and antigen in subacute sclerosing panencephalitis. Lancet 1:542–544
    [Google Scholar]
  8. Cotton R. G. H., Secher D. S., Milstein C. 1973; Somatic mutation and the origin of antibody diversity. Clonal variability of the immunoglobulin produced by MOPC21 cells in culture. European Journal of Immunology 3:135–140
    [Google Scholar]
  9. Holland J. J., Graban E. A., Jones C. L., Semler B. L. 1979; Evolution of multiple genome mutations during long-term persistent infections by vesicular stomatitis virus. Cell 16:495–504
    [Google Scholar]
  10. Horta-Barbosa L., Fuccillo D. A., Sever J. L., Zeman W. 1969; Subacute sclerosing panencephalitis: isolation of measles virus from a brain biopsy. Nature, London 221:947
    [Google Scholar]
  11. Horta-Barbosa L., Hamilton L. R., Witting B. W., Fuccillo D. A., Sever J. L., Vernon M. L. 1971; Subacute sclerosing panencephalitis: isolation of suppressed measles virus from lymph node biopsies. Science 173:840–841
    [Google Scholar]
  12. Kohler G., Milstein C. 1975; Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256:495–497
    [Google Scholar]
  13. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  14. Levy N. L., Auerback P. S., Hayes E. C. 1976; A blood test for multiple sclerosis based on the adherence of lymphocytes to measles-infected cells. New England Journal of Medicine 294:1423–1427
    [Google Scholar]
  15. McFarlin D. E., Bellini W. J., Mingioli E. S., Behar T. N., Trudgett A. 1980; Monospecific antibody to the haem agglutinin of measles virus. Journal of General Virology 48:425–429
    [Google Scholar]
  16. Merz D. C., Scheid A., Choppin P. W. 1980; Importance of antibodies to the fusion glycoprotein of paramyxoviruses in the prevention of spread of infection. Journal of Experimental Medicine 151:275–288
    [Google Scholar]
  17. Norrby R., Gollmar Y. 1975; Identification of measles virus-specific hemolysis inhibiting antibodies separate from hemagglutination inhibiting antibodies. Infection and Immunity 11:231–239
    [Google Scholar]
  18. Payne F. E., Baublis J. V. 1973; Decreased reactivity of SSPE strains of measles virus with antibody. Journal of Infectious Diseases 127:505–511
    [Google Scholar]
  19. Schneider E. L., Stanbridge E. J., Epstein C. J. 1974; Incorporation of 3H-uridine and 3H-uracil into RNA. A simple technique for the detection of mycoplasma contamination of cultured cells. Experimental Cell Research 84:311–318
    [Google Scholar]
  20. Wild T. F., Dugre R. 1978; Establishment and characterization of a subacute sclerosing panencephalitis (measles) virus persistent infection in BGM cells. Journal of General Virology 39:113–124
    [Google Scholar]
  21. Wild T. F., Huppert J. 1980; Specificity of measles and canine distemper virus antibodies. Annales de Vlnstitut Pasteur 131E:73–84
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-54-2-325
Loading
/content/journal/jgv/10.1099/0022-1317-54-2-325
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error