1887

Abstract

SUMMARY

Feline rotavirus was detected by electron microscopy in the faecal samples of a cat, and was propagated in an established cell line of foetal rhesus monkey kidney, MA104, cell cultures. Morphologically, feline rotavirus was indistinguishable from known rotaviruses. Complete particles showed a characteristic ‘spoke-like’ arrangement of inner capsomeres surrounded by an outer layer. Intracytoplasmic inclusion bodies in different sizes and shapes were produced in infected MA104 cells. Reproducible clear-cut plaques were produced by feline rotavirus in MA104 cells under the overlay of carboxymethylcellulose in the presence of trypsin. Feline rotavirus was distinct from human, canine, bovine, porcine and simian rotaviruses by the plaque reduction neutralization test. Feline rotavirus, like canine and simian rotaviruses, was found to be less dependent upon trypsin than human, bovine, porcine, chicken and turkey rotaviruses. A seroepidemiological survey (September 1979 to August 1980) showed that 20 out of 61 (32.8%) randomly sampled hospitalized cats at the Cornell Veterinary Teaching Hospital in Ithaca, New York had antibody titres against feline rotavirus. Oral inoculation of cats with feline rotavirus did not produce any clinical disease, but most cats did mount an immune response to the virus following inoculation.

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1981-06-01
2021-10-27
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References

  1. Almeida J. D., Hall T. E., Banatvala J. E., Totterdell B. M., Chrystie I. L. 1978; The effect of trypsin On the growth of rotaviruses. Journal of General Virology 40:213–218
    [Google Scholar]
  2. Babiuk L. A., Mohammed K., Spence L., Fauvel M., Petro R. 1977; Rotavirus isolation and cultivation in the presence of trypsin. Journal of Clinical Microbiology 6:610–617
    [Google Scholar]
  3. Barnett B. B., Spendlove R. S., Clark M. L. 1979; Effect of enzymes on rotavirus infectivity. Journal of Clinical Microbiology 10:111–113
    [Google Scholar]
  4. Bergeland M. E., McAdaragh J. P., Stotz I. 1977; Rotaviral enteritis in turkey poults. Proceedings of the 26 th Western Poultry Disease Conference, University of California, Davis pp 129–130
    [Google Scholar]
  5. Bridger J. C., Woode G. N., Jones J. M., Flewett T. H., Brydent A. S., Davies H. 1975; Transmission of human rotaviruses to gnotobiotic piglets. Journal of Medical Microbiology 8:565–569
    [Google Scholar]
  6. Chrystie I. L., Goldwater P. N., Banatvala J. E. 1979; Rotavirus infection in a domestic cat. Veterinary Record 105:404–405
    [Google Scholar]
  7. Clark S. M., Barnett B. B., Spendlove R. S. 1979; Production of high-titer bovine rotavirus with trypsin. Journal of Clinical Microbiology 9:413–417
    [Google Scholar]
  8. England J. J., Poston B. S. 1980; Electron microscopic identification and subsequent isolation of a rotavirus from a dog with fatal noenatal diarrhea. American Journal of Veterinary Research 41:781–783
    [Google Scholar]
  9. Esparza J., Gorziglia M., Gil F., Romer H. 1980; Multiplication of human rotavirus in cultured cells: an electron microscopic study. Journal of General Virology 47:461–472
    [Google Scholar]
  10. Eugster A. K., Sneed L. 1980; Viral intestinal infections of animals and man. Comparative Immunology Microbiology and Infectious Diseases 2:417–435
    [Google Scholar]
  11. Flewett T. H., Woode G. N. 1978; The rotaviruses. Archives of Virology 57:1–23
    [Google Scholar]
  12. Graham D. Y., Estes M. K. 1980; Proteolytic enhancement of rotavirus infectivity: biologic mechanisms. Virology 101:432–439
    [Google Scholar]
  13. Holmes I. H. 1979; Viral gastroenteritis. Progress in Medical Virology 25:1–36
    [Google Scholar]
  14. Hoshino Y., Scott F. W. 1980; Coronavirus-like particles in the feces of normal cats. Archives of Virology 63:147–152
    [Google Scholar]
  15. Jones R. C., Hughes C. S., Henry R. R. 1979; Rotavirus infection in commercial laying hens. Veterinary Record 104:22
    [Google Scholar]
  16. Kapikian A. Z., Wyatt R. G., Greenberg H. B., Kalica A. R., Kim H. W., Brandt C. D., Rodriguez W. J., Parrot R. H., Chanock R. M. 1980; Approaches to immunization of infants and young children against gastroenteritis due to rotaviruses. Reviews of Infectious Diseases 2:459–469
    [Google Scholar]
  17. McNulty M. S. 1978; Rotaviruses. Journal of General Virology 40:1–18
    [Google Scholar]
  18. McNulty M. S., Allan G. M., Stuart J. C. 1978a; Rotavirus infection in avian species. Veterinary Record 103:319–320
    [Google Scholar]
  19. McNulty M. S., Allan G. M., Thompson D. J., O’Boyle J. D. 1978b; Antibody to rotavirus in dogs and cats. Veterinary Record 102:534–535
    [Google Scholar]
  20. McNulty M. S., Allan G. M., Todd D., Mcferran J. B. 1979; Isolation and cell culture propagation of rotaviruses from turkeys and chickens. Archives of Virology 61:13–21
    [Google Scholar]
  21. Malherbe H. H., Strickland-Cholmley M. 1967; Simian virus SA11 and the related O agent. Archiv.fiir die gesamte Virusforschung 22:235–245
    [Google Scholar]
  22. Mebus C. A., Wyatt R. G., Sharpee R. L., Sereno M. M., Kalica A. R., Kapikian A. Z., Twiehaus M. J. 1976; Diarrhea in gnotobiotic calves caused by the rotavirus-like agent of human infantile gastroenteritis. Infection and Immunity 14:471–474
    [Google Scholar]
  23. Middleton P. J., Petric M., Szymanski M. T. 1975; Propagation of infantile gastroenteritis virus (orbi-group) in conventional and germ free piglets. Infection and Immunity 12:1276–1280
    [Google Scholar]
  24. Ramia S., Sattar S. A. 1979; Simian rotavirus SA-11 plaque formation in the presence of trypsin. Journal of Clinical Microbiology 10:609–614
    [Google Scholar]
  25. Ramia S., Sattar S. A. 1980; Proteolytic enzymes and rotavirus SA-11 plaque formation. Canadian Journal of Comparative Medicine 44:232–236
    [Google Scholar]
  26. Rodger S. M., Schnagl R. D., Holmes I. H. 1977; Further biochemical characterization, including the detection of surface glycoproteins, of human, calf, and simian rotaviruses. Journal of Virology 24:91–98
    [Google Scholar]
  27. Schoub B. D., Kalica A. R., Greenberg H. B., Bertran D. M., Sereno M. M., Wyatt R. G., Chanock R. M., Kapikian A. Z. 1979; Enhancement of antigen incorporation and infectivity of cell culture by human rotavirus. Journal of Clinical Microbiology 9:488–492
    [Google Scholar]
  28. Smith E. M., Estes M. K., Graham D. Y., Gerba C. P. 1979; A plaque assay for the simian rotavirus SA-11. Journal of General Virology 43:513–519
    [Google Scholar]
  29. Snodgrass D. R., Madeley C. R., Wells P. W., Angus K. W. 1977; Human rotavirus in lambs: infection and passive protection. Infection and Immunity 16:268–270
    [Google Scholar]
  30. Soike K. G., Gary G. W., Gibson S. 1980; Susceptibility of non-human primate species to infection by simian rotavirus SA-11. American Journal of Veterinary Research 41:1098–1103
    [Google Scholar]
  31. Theil K. W., Bohl E. H., Agnes A. G. 1977; Cell culture propagation of porcine rotavirus (reovirus-like agent). American Journal of Veterinary Research 38:1765–1768
    [Google Scholar]
  32. Thouless M. E., Bryden A. S., Flewett T. H., Woode G. N., Gridger J. C., Snodgrass D. R., Herring J. A. 1977; Serological relationships between rotaviruses from different species as studied by complement fixation and neutralization. Archives of Virology 53:287–294
    [Google Scholar]
  33. Torres-Medina A., Wyatt R. G., Mebus C. A., Underdahl N. R., Kapikian A. Z. 1976; Diarrhea caused in gnotobiotic piglets by the reovirus-like agent of human infantile gastroenteritis. Journal of Infectious Diseases 133:22–27
    [Google Scholar]
  34. Welch A. B., Twiehaus M. J. 1973; Cell culture studies of a neonatal calf diarrhea virus. Canadian Journal of Comparative Medicine 37:287–294
    [Google Scholar]
  35. Wyatt R. G., Kalica A. R., Mebus C. A., Kim H. W., London W. T., Chanock R. M., Kapikian A. Z. 1978; Reovirus-like agents (rotaviruses) associated with diarrheal illness in animals and man. In Perspectives in Virology vol 10: pp 121–145 Edited by Pollard M. New York: Raven Press;
    [Google Scholar]
  36. Wyatt R. G., James W. D., Bohl E. H., Theil K. W., Saif L. J., Kalica A. R., Greenberg H. B., Kapikian A. Z., Chanock R. M. 1980; Human rotavirus type 2: cultivation in vitro. Science 207:189–191
    [Google Scholar]
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