1887

Abstract

SUMMARY

A reovirus-like agent has been isolated from an established cell line 67j25D-G. The virion was 68 nm in diam. with a distinct outer capsid. The virus particles contained at least five polypeptides with mol. wt. of 30, 40, 70, 100 and 120 (all × 10) and 10 segments of double-stranded high mol. wt. RNA. Viruses of the same type were detected in several cell cultures of obtained from different sources. The viral nucleic acid accounted for about 20% of the RNA in the cultured cells.

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1981-05-01
2021-10-18
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References

  1. Agol V. I., Romanova L. I., Cumakov I. M., Dunaevskaya L. D., Bogdanov A. A. 1972; Circularity and cross-linking in preparations of replicative form of encephalomyocarditis virus RNA. Journal of Molecular Biology 72:77–89
    [Google Scholar]
  2. Barnes W. M. 1977; Plasmid detection and sizing in single colony lysates. Science 195:393–394
    [Google Scholar]
  3. Gateff E. 1978; The genetics and epigenetics of neoplasms in Drosophila. Biological Reviews and Biological Proceedings of the Cambridge Philosophical Society 53:123–168
    [Google Scholar]
  4. Haars R., Zentgraf H., Gateff E., Bautz F. A. 1980; Evidence for endogenous reovirus-like particles in a tissue cultured cell line from Drosophila melanogaster. Virology 101:124–130
    [Google Scholar]
  5. Hirumi H. 1976; Viral, microbial and extrinsic cell contamination of insect cell cultures. In Invertebrate Tissue Culture pp 233–268 Edited by Maramorosch K. New York and London: Academic Press;
    [Google Scholar]
  6. Jousset F. X., Bergoin M. 1977; Characterization of the Drosophila C virus. Journal of General Virology 34:269–285
    [Google Scholar]
  7. Kakpakov V. T., Gvozdev V. A., Platova T. P., Polukarova L. G. 1969; In vitro establishment of embryonic cell lines of Drosophila melanogaster. Genetika 5:67–75 (Soviet Genetics 5. 1647-1655)
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  9. Lengyel J., Spradling A., Penman S. 1975; Methods with insect cells in suspension culture. II. Drosophila melanogaster. Methods in Cell Biology 10:195–208
    [Google Scholar]
  10. Mckenzie S. L., Henikopf S., Meselson M. 1975; Localization of RNA from heat induced polysomes at puff sites in Drosophila melanogaster. Proceedings of the National Academy of Sciences of the United States of American 72:1117–1121
    [Google Scholar]
  11. Metakovskii E. V., Kakpakov V. T., Gvozdev V. A. 1975; Effect of ecdysterone on the culture of established cell line of Drosophila melanogaster: enhancement of high molecular polypeptide synthesis and the change in surface properties of cells. Doklady of the Academy of Sciences of the U,. S,. S,. R 221:960–963
    [Google Scholar]
  12. Nermut M. V. 1973; Freeze-drying and freeze-etching of viruses. In Freeze-etching Techniques and Applications pp 135–171 Edited by Favard P., Benedetti E. L. Paris: Societe Franijaise de Microscopie Electronique;
    [Google Scholar]
  13. Plus N. 1978; Endogenous viruses of Drosophila melanogaster cell lines: their frequency, identification and origin. In vitro 14:1015–1021
    [Google Scholar]
  14. Plus N., Croizier G., Jourset F. X., David J. 1975a; Picornaviruses of laboratory and wild Drosophila melanogaster: geographical distribution and serotypic composition. Annales de Microbiologie, Institut Pasteur 126 A:107–117
    [Google Scholar]
  15. Plus N., Croizier G., Duthoit C. L., David J., Anxolabehere D., Periquet G. 1975b; Decouverte, chez la Drosophila, de virus appartenant a trois nouveaux groupes. Comptes rendus hebdomadaires des seances de IʼAcademie des Sciences, Series D 280:1501–1504
    [Google Scholar]
  16. Plus N., Croizier G., Veyrunes J. C., David J. 1976; A comparison of buoyant density and polypeptides of Drosophila P, C and A viruses. Intervirology 7:346–350
    [Google Scholar]
  17. Ramig R. F., Fields B. N. 1977; Reoviruses. In The Molecular Biology of Animal Viruses vol 1 pp 383–433 Edited by Nayak D. New York and Basel: Marcel Dekker;
    [Google Scholar]
  18. Schneider I., Blumenthal A. B. 1978; Drosophila cell and tissue culture. In Genetics and Biology of Drosophila vol 2A pp 266–315 Edited by Ashburner M., Wright T. R. F. London: Academic Press;
    [Google Scholar]
  19. Sharpe A. H., Ramig R. F., Murtoc T. A., Fields B. N. 1978; A genetic map of reovirus. 1. Correlation of genomic RNAs between serotypes 1. 2, 3. Virology 84:63–74
    [Google Scholar]
  20. Shatkin A. J. 1965; Actinomycin and the differential synthesis of reovirus and L cell RNA. Biochemical and Biophysical Research Communications 19:506–510
    [Google Scholar]
  21. Teninges D., Ohanessian A., Richard Molard Ch., Contamine D. 1979a; Isolation and biological properties of the Drosophila X virus. Journal of General Virology 42:241–254
    [Google Scholar]
  22. Teninges D., Ohanessian A., Richard-Molard Ch., Contamine D. 1979b; Contamination and persistent infection of Drosophila cell lines by reovirus type particles. In Vitro 15:425–428
    [Google Scholar]
  23. Williamson D. L., Kernaghan R. P. 1972; Virus-like particles in Schneider’s Drosophila cell lines. Drosophila Information Service 48:58–59
    [Google Scholar]
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