RT Journal Article SR Electronic(1) A1 Wege, Helmut A1 Siddell, Stuart A1 Sturm, Margarete A1 ter Meulen, VolkerYR 1981 T1 Coronavirus JHM: Characterization of Intracellular Viral RNA JF Journal of General Virology, VO 54 IS 1 SP 213 OP 217 DO https://doi.org/10.1099/0022-1317-54-1-213 PB Microbiology Society, SN 1465-2099, AB SUMMARY After infection of Sac(-) cells with the murine coronavirus JHM the synthesis of seven major and two minor RNA species was induced. These RNAs were polyadenylated and single-stranded. Their mol. wt. were estimated by electrophoresis in agarose gels containing methylmercury hydroxide. The values for the major species were 6.67 × 106 for RNA of genome size (RNA 1), 3.42 × 106 for RNA 2, 2.76 × 106 for RNA 3, 1.35 × 106 for RNA 4, 1.19 × 106 for RNA 5, 0.93 × 106 for RNA 6 and 0.62 × 106 for RNA 7. The minor species have a size of 4.7 × 106 (RNA a) and 1.5 × 106 (RNA b). The same number of species were found by electrophoresis after denaturation with glyoxal-dimethyl sulphoxide. No gross difference in number of RNAs and the amount of each species was found between total cytoplasmic RNA, polyadenylated cytoplasmic RNA and RNA extracted from pelleted polysomes., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-54-1-213