RT Journal Article SR Electronic(1) A1 Koide, Norio A1 Wells, Alan A1 Volsky, David J. A1 Shapiro, Ilya M. A1 Klein, GeorgeYR 1981 T1 The Detection of Epstein—Barr Virus Receptors Utilizing Radiolabelled Virus JF Journal of General Virology, VO 54 IS 1 SP 191 OP 195 DO https://doi.org/10.1099/0022-1317-54-1-191 PB Microbiology Society, SN 1465-2099, AB SUMMARY Epstein—Barr virus (EBV) was labelled with 3H-thymidine and purified about 1000-fold from the culture medium by ultracentrifugation on 5 to 30% dextran grandients. The presence of the virus was monitored by radioactivity and Epstein—Barr virus-determined nuclear antigen (EBNA) induction in sensitive indicator cells (Ramos). Peaks for both activities occurred in the 17 to 18% dextran fractions. Unlabelled virus recovered in the peak fraction was labelled with 125I. Both thymidine and 125I-labelled purified virus bound quantitatively to receptor-positive Burkitt lymphoma-derived cell lines but not to EBV-receptor-negative T-lymphocyte-derived cell lines. Thymidine-labelled virus that was allowed to bind to Raji cells was present in the interior of briefly trypsinized cells after 3 h incubation at 37 °C. The results provide a convenient method for detecting the EBV receptor by radioactively labelled virus., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-54-1-191