1887

Abstract

SUMMARY

Structural proteins from a large-plaque variant (LPV) of human parainfluenza type 3 virus were analysed by electrophoresis on Laemmli-type polyacrylamide gels. High virus concentrations were obtained by growth in BS-C-1 cells cultivated on microcarrier beads. Purification of the virus in composite equilibrium gradients of potassium tartrate:glycerol resulted in 25% recovery of input infectivity and a preparation containing <0.08% of input host cell protein and RNA. Parainfluenza type 3 virus equilibrated at a density of 1.20 g/ml in these gradients. Analysis by polyacrylamide gel electrophoresis of H-glucosamine-labelled virus taken from peak gradient fractions revealed 8 or 9 major virion peptides, ranging in mol. wt. from 17 × 10 to 125 × 10 (17K to 125K), two of which were glycoproteins. The sum of the estimated mol. wt. of these peptides, 501.5K to 570.5K, does not exceed the estimated genomic potential of other paramyxoviruses.

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1981-05-01
2022-01-23
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References

  1. Bablanian R., Eggers H. J., Tamm I. 1965; Studies on the mechanism of poliovirus-induced cell damage. I. The relation between poliovirus-induced metabolic and morphological alterations in cultured cells. Virology 26:100–113
    [Google Scholar]
  2. Buetti E., Choppin P. W. 1977; The transcriptase complex of the paramyxovirus SV-5. Virology 82:493–508
    [Google Scholar]
  3. Chanock R. M., Parrott R. H., Johnson K. M., Kapikian A. Z., Bell J. A. 1963; Myxoviruses: parainfluenza. American Review of Respiratory Disease 88:152–166
    [Google Scholar]
  4. Choppin P. W., Scheid A. 1980; The role of viral glycoproteins in adsorption, penetration, and pathogenicity of viruses. Review of Infectious Diseases 2:0–61
    [Google Scholar]
  5. Colonno R. J., Stone H. O. 1976; Isolation of a transcriptive complex from Newcastle disease virions. Journal of Virology 19:1080–1089
    [Google Scholar]
  6. Dick E. C., Mogabgab W. J. 1962; Characteristics of parainfluenza I (HA-2) virus. III. Antigenic relationships, growth, interactions with erythrocytes, and physical properties. Journal of Bacteriology 83:561–571
    [Google Scholar]
  7. Gelzen W. P., Loda F. A., Denny F. W. 1976; The parainfluenza virus. In Viral Infections of Humans, Epidemiology, and Control pp 337–349 Edited by Evans A. S. New York: Plenum Press;
    [Google Scholar]
  8. Homma M., Ohuchi M. 1973; Trypsin action on the growth of Sendai virus in tissue culture cells. III. Structural difference of Sendai viruses grown in eggs and tissue culture cells. Journal of Virology 12:457–1465
    [Google Scholar]
  9. Hsu M. C., Scheid A., Choppin P. W. 1979; Reconstitution of membranes with individual paramyxovirus glycoproteins and phospholipid in cholate solution. Virology 95:476–491
    [Google Scholar]
  10. Kingsbury D. W. 1977; Paramyxoviruses. In The Molecular Biology of Animal Viruses vol 1: pp 349–382 Edited by Nayak D. New York: Marcel Dekker;
    [Google Scholar]
  11. Klenk H.-D., Choppin P. W. 1969; Chemical composition of the parainfluenza virus SV-5. Virology 37:155–157
    [Google Scholar]
  12. Klenk H.-D., Caliguiri L. A., Choppin P. W. 1970; The proteins of the parainfluenza virus SV-5. II. The carbohydrate content and glycoproteins of the virion. Virology 38:225–268
    [Google Scholar]
  13. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  14. Lamb R. A., Mahy B. W. J. 1975; The polypeptides and RNA of Sendai virus. In Negative Strand Viruses vol 1: pp 65–87 Edited by Mahy B. W. J., Barry R. D. London: Academic Press;
    [Google Scholar]
  15. Lamb R. A., Mahy B. W. J., Choppin P. W. 1976; The synthesis of Sendai virus polypeptides in infected cells. Virology 69:116–131
    [Google Scholar]
  16. Levine D. W., Wong J. S., Wang D. I. C., Thilly W. G. 1977; Microcarrier cell culture: new methods for research-scale application. Somatic Cell Genetics 3:149–155
    [Google Scholar]
  17. Mandel B. 1958; Studies on the interactions of poliomyelitis virus, antibody, and host cells in a tissue culture system. Virology 6:424–447
    [Google Scholar]
  18. Mountcastle W. E., Compans R. W., Choppin P. W. 1971; Proteins and glycoproteins of paramyxoviruses: a comparison of simian virus 5. Newcastle disease virus, and Sendai virus. Journal of Virology 7:47–52
    [Google Scholar]
  19. Nagai Y., Klenk H.-D., Rott R. 1976a; A proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus. Virology 72:494–508
    [Google Scholar]
  20. Nagai Y., Ogura H., Klenk H.-D. 1976b; Studies on the assembly of the envelope of Newcastle disease virus. Virology 69:523–538
    [Google Scholar]
  21. Obueski J. F., Marchenko A. T., Bishop D. H. L., Cann B. W., Murphy F. A. 1974; Comparative electrophoretic analysis of the virus proteins for four rhabdoviruses. Journal of General Virology 22:21–33
    [Google Scholar]
  22. Scheid A., Choppin P. W. 1973; Isolation and purification of the envelope proteins of Newcastle disease virus. Journal of Virology 11:263–271
    [Google Scholar]
  23. Scheid A., Choppin P. W. 1974; Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis and infectivity by proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology 57:75–490
    [Google Scholar]
  24. Scheid A., Choppin P. W. 1977; Two disulfide-linked polypeptide chains constitute the active F protein of paramyxoviruses. Virology 80:54–66
    [Google Scholar]
  25. Scheid A., Caliguiri L. A., Compans R. W., Choppin P. W. 1972; Isolation of paramyxovirus glycoproteins. Association of both hemagglutinin and neuraminidase activities with the larger SV-5 glycoprotein. Virology 50:640–652
    [Google Scholar]
  26. Shibuta H., Kanda T., Adachi A., Yogo Y. 1979; Characterization of bovine parainfluenza virus type 3. Microbiology and Immunology 23:17–628
    [Google Scholar]
  27. Tozawa H., Watanabe M., Ishida N. 1973; Structural components of Sendai virus. Serological and physicochemical characterization of hemagglutinin subunit associated with neuraminidase activity. Virology 55:242–253
    [Google Scholar]
  28. Weber K., Osborne M. 1969; The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry 224:406–4412
    [Google Scholar]
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