Absence of Activated Murine Leukaemia Virus in X-irradiated CBA/H-T6Crc Mice Free

Abstract

SUMMARY

CBA/H-T6Crc mice, a substrain that does not normally express demonstrable levels of murine leukaemia virus (MuLV) and has a low natural incidence of leukaemia, were examined for evidence of virus activation at various times following X-irradiation. Although X-irradiation caused a high incidence of leukaemia, no ecotropic, xenotropic or recombinant MuLV was detected by co-cultivation of bone marrow, spleen and thymus cells from pre-leukaemic and leukaemic animals with selectively permissive cell lines followed by indirect immunofluorescence for MuLV group-specific (gs) antigen. These results, therefore, are not consistent with the hypothesis that endogenous viruses are the universal aetiological agents of leukaemia.

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1981-04-01
2024-03-29
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References

  1. Arnstein P., Rriggs J. L., Oshiro L. S., Huebner R. I., Lennette E. H. 1976; Induction of lymphoma and associated xenotropic type C virus in C57L mice by whole body irradiation. Journal of the National Cancer Institute 57:1085–1090
    [Google Scholar]
  2. Barnes R. D., Tuffrey M. 1974; Absence of lymphomas in CBA mice derived by embryo transfer and born from lymphoma-prone AKR mice. European Journal of Cancer 10:575–578
    [Google Scholar]
  3. Ellis R. W., Stockert E., Fleissner E. 1980; Association of endogenous retroviruses with radiation induced leukaemias of BALB/c mice. Journal of Virology 33:652–660
    [Google Scholar]
  4. Gisselbrecht S., Bassin R. H., Gerwin B. I., Rein A. 1974; Dual susceptibility of a 3T3 mouse cell line to infection by N- and B-tropic murine leukemia virus: apparent lack of expression of the FV-1 gene. International Journal of Cancer 14:106–113
    [Google Scholar]
  5. Gross L. 1951; ‘Spontaneous’ leukaemia developing in C3H mice following inoculation, in infancy, with AK-leukaemic extracts or AK-embryos. Proceedings of the Society for Experimental Biology and Medicine 76:27–32
    [Google Scholar]
  6. Haas M. 1977; Transient virus expression during murine leukemia induction by X-irradiation. Journal of the National Cancer Institute 58:251–257
    [Google Scholar]
  7. Haas M. 1978; Leukemogenic activity of thymotropic, ecotropic and xenotropic radiation leukemia virus isolates. Journal of Virology 25:705–709
    [Google Scholar]
  8. Haran-ghera N. 1966; Leukaemogenic activity of centrifugates from irradiated mouse thymus and bone marrow. International Journal of Cancer 1:81–87
    [Google Scholar]
  9. Hartley J. W., Rowe W. P. 1975; Clonal cell lines from a feral mouse embryo which lack host-range restrictions for murine leukemia viruses. Virology 65:128–134
    [Google Scholar]
  10. Hartley J. W., Wolford N. K., Old L. J., Rowe W. P. 1977; A new class of murine leukemia virus associated with development of spontaneous lymphomas. Proceedings of the National Academy of Sciences of the United States of America 74:789–792
    [Google Scholar]
  11. Harvey J. J., Tuffrey M., Holmes H. C., East J. 1979; Absence of ecotropic or recombinant murine leukaemia virus in preleukaemic and leukaemic x irradiated NZB mice. International Journal of Cancer 24:373–376
    [Google Scholar]
  12. Henderson I. C., Lieber M. M., Todaro G. J. 1974; Mink cell line MvlLu (CCL 64). Focus formation and the generation of ‘nonproducer’ transformed cell lines with murine and feline sarcoma viruses. Virologv 60:282–287
    [Google Scholar]
  13. Hilgers J., Nowinski R. C., Geering G., Hardy W. 1972; Detection of avian and mammalian oncogenic RNA viruses (oncornaviruses) by immunofluorescence. Cancer Research 32:98–106
    [Google Scholar]
  14. Ihle J. N. 1977; Serological and biochemical characterization of ecotropic MuLV expression in radiation-induced leukemias of mice. In INSERM Symposium No. 4 pp 265–274 Edited by Duplan F. Amsterdam: North Holland Biomedical Press;
    [Google Scholar]
  15. Ihle J. N., Joseph D. R., Pazmino N. H. 1976; Radiation leukemia in C57BL/6 mice. II. Lack of ecotropic virus expression in the majority of lymphomas. Journal of Experimental Medicine 144:1406–1423
    [Google Scholar]
  16. Kaplan H. S. 1978; From experimental animal models to human lymphoid tissue neoplasia: search for a viral etiology. Recent Results in Cancer Research 64:325–336
    [Google Scholar]
  17. Kaplan H. S., Brown M. B. 1952; Mortality of mice after total body irradiation as influenced by alterations in total dose, fractionation and periodicity of treatment. Journal of the National Cancer Institute 12:765–775
    [Google Scholar]
  18. Kawashima K., Ikeda H., Hartley J. W., Stockert E., Rowe W. P., Old L. J. 1976; Changes in expression of murine leukaemia virus antigens and production of xenotropic virus in the late preleukaemic period in AKR mice. Proceedings of the National Academy of Sciences of the United States of America 73:4680–4684
    [Google Scholar]
  19. Lieberman M., Kaplan H. S. 1959; Leukemogenic activity of filtrates from radiation-induced lymphoid tumors of mice. Science 130:387–388
    [Google Scholar]
  20. Lieberman M., Decleve A., Ihle J. N., Kaplan H. S. 1979; Rescue of a thymotropic, leukaemogenic C-type virus from cultured, non-producer lymphoma cells of strain C57BL/ka mice. Virology 97:12–21
    [Google Scholar]
  21. Melief C. J. M., Datta S. K., Louie S., Johnson S., Melief M., Schwartz R. S. 1975; Splenocyte plaque assay for the detection of murine leukemia virus. Proceedings of the Society for Experimental Biology and Medicine 149:1015–1018
    [Google Scholar]
  22. Vogt M. 1979; Properties of mink cell focus-inducing (MCF) virus isolated from spontaneous lymphoma lines of BALB/c mice carrying moloney leukaemia virus as an endogenous virus. Virology 93:226–236
    [Google Scholar]
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