1887

Abstract

SUMMARY

Bovine rotavirus was found to multiply efficiently in LLC-MK cells, a continuous line of rhesus monkey kidney, with a growth cycle which was essentially completed within 9 h after infection. The presence of low concentrations of trypsin (10 µg/ml) in the virus inoculum was essential for infectivity. Polyacrylamide gel electrophoresis of infected cell extracts demonstrated the synthesis of at least eight virus-specific polypeptides 6 h post-infection with mol. wt. ranging from 102 × 10 to 29 × 10. Six polypeptides (about p102K, p91K, p84K, p45K, p37K and p34K) were identified as structural components of the virion. Two other polypeptides (p54K and p29K) were identified as non-structural components. The synthesis of non-structural polypeptides appeared to precede that of the structural proteins. Pulse-chase experiments showed only one minor post-translation modification of the virus-specified proteins, namely an increase in the mobility of the 29K polypeptide.

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1981-04-01
2022-01-21
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References

  1. Almeida J. D., Hall T., Banatvala J. E., Totterdell B. M., Chrystie I. L. 1978; The effect of trypsin On the growth of rotavirus. Journal of General Virology 40:213–218
    [Google Scholar]
  2. Babiuk L. A., Mohammed K., Spence L., Fauvel M., Petro R. 1977; Rotavirus isolation and cultivation in the presence of trypsin. Journal of Clinical Microbiology 6:610–617
    [Google Scholar]
  3. Barnett B. B., Splendove R. S., Clark M. L. 1979; Effect of enzymes on rotavirus infectivity. Journal of Clinical Microbiology 10:111–113
    [Google Scholar]
  4. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  5. Bridger J. C., Woode G. N. 1976; Characterization of two particle types of calf rotavirus. Journal of General Virology 31:245–250
    [Google Scholar]
  6. Clark S. M., Barnett B. B., Splendove R. S. 1979; Production of high-titer bovine rotavirus with trypsin. Journal of Clinical Microbiology 9:413–417
    [Google Scholar]
  7. Cohen J. 1977; Ribonucleic acid polymerase activity associated with purified calf rotavirus. Journal of General Virology 36:395–402
    [Google Scholar]
  8. Cohen J., Laporte J., Charpilienne A., Scherrer R. 1979; Activation of rotavirus RNA polymerase by calcium chelation. Archives of Virology 60:177–186
    [Google Scholar]
  9. Cross R. K., Fields B. 1976; Reovirus-specific polypeptides: analysis using discontinuous gel electrophoresis. Journal of Virology 19:162–173
    [Google Scholar]
  10. Esparza J., Gil F. 1978; A study on the ultrastructure of human rotavirus. Virology 91:141–150
    [Google Scholar]
  11. Esparza J., Gorziglia M., Gil F., Romer H. 1980; Multiplication of human rotavirus in cultured cells: an electron microscopic study. Journal of General Virology 47:461–472
    [Google Scholar]
  12. Estes M. K., Graham D. Y., Gerba C. P., Smith E. W. 1979; Simian rotavirus SA11 replication in cell cultures. Journal of Virology 31:810–815
    [Google Scholar]
  13. Flewett T. H., Woode G. N. 1978; The rotaviruses. Archives of Virology 57:1–23
    [Google Scholar]
  14. Gorziglia M., Esparza J. 1981; Poly (A) polymerase activity in human rotavirus. Journal of General Virology 53:357–362
    [Google Scholar]
  15. Honess R. W., Roizman B. 1973; Proteins specified by herpes simplex virus. XI. Identification and relative molar rates of synthesis of structural and nonstructural herpes virus polypeptides in the infected cell. Journal of Virology 12:1347–1365
    [Google Scholar]
  16. Hruska J. F., Notter M. F. D., Menegus M. A., Steinhoff M. C. 1978; RNA polymerase associated with human rotaviruses in diarrhea stools. Journal of Virology 26:544–546
    [Google Scholar]
  17. Kalica A. R., Sereno M. M., Wyatt R. G., Mebus C. A., Chanock R. M., Kapikian A. Z. 1978; Comparison of human and animal rotavirus strains by gel electrophoresis of viral RNA. Virology 87:247–255
    [Google Scholar]
  18. Kapikian A. Z., Yolken R. H., Wyatt R. G., Kalica A. R., Chanock R. M., Kim H. W. 1978; Viral diarrhea. Etiology and control. American Journal of Clinical Nutrition 31:2219–2236
    [Google Scholar]
  19. Krystal G., Perrault J., Graham A. F. 1976; Evidence for a glycoprotein in reovirus. Virology 72:308–321
    [Google Scholar]
  20. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  21. Laskey R. A., Mills A. D. 1975; Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  22. Matsuno S., Mukoyama A. 1979; Polypeptides of bovine rotavirus. Journal of General Virology 43:309–316
    [Google Scholar]
  23. Matsuno S., Inouye S., Kono R. 1977; Plaque assay of neonatal calf diarrhea virus and the neutralizing antibody in human sera. Journal of Clinical Microbiology 5:1–4
    [Google Scholar]
  24. Mebus C. A., Kono M., Underdahl N. R., Twiehaus M. J. 1971; Cell culture propagation of neonatal calf diarrhea (scours) virus. Canadian Veterinary Journal 12:69–72
    [Google Scholar]
  25. Newman J. F. E., Brown F., Bridger J. C., Woode G. N. 1975; Characterization of a rotavirus. Nature, London 258:631–633
    [Google Scholar]
  26. Rodger S. M., Holmes I. H. 1979; Comparison of the genomes of simian, bovine, and human rotaviruses by gel electrophoresis and detection of genomic variation among bovine isolates. Journal of Virology 30:839–846
    [Google Scholar]
  27. Rodger S. M., Schnagl R. D., Holmes I. H. 1977; Further biochemical characterization, including the detection of surface glycoproteins, of human, calf and simian rotaviruses. Journal of Virology 24:91–98
    [Google Scholar]
  28. Schoub B. D., Kalica A. R., Greenberg H. B., Bertran D. M., Sereno M. M., Wyatt R. G., Chanock R. M., Kapikian A. Z. 1979; Enhancement of antigen incorporation and infectivity of cell cultures by human rotavirus. Journal of Clinical Microbiology 9:488–492
    [Google Scholar]
  29. Thouless M. E. 1979; Rotavirus polypeptides. Journal of General Virology 44:187–197
    [Google Scholar]
  30. Viera De Torres B., Mazzali De Iua R., Esparza J. 1978; Epidemiological aspects of rotavirus infection in hospitalized Venezuelan children with gastroenteritis. American Journal of Tropical Medicine and Hygiene 27:567–572
    [Google Scholar]
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