1887

Abstract

Summary

A poly(A) polymerase enzymic activity was found in partially purified preparations of a human rotavirus. The activity was demonstrated using conditions similar to those utilized for the detection of a poly(A) polymerase previously described in reovirus (incubation at 43 °C in 70 m-tris buffer pH 7.5 containing 12 m·Mn). The enzymic activity was associated only with complete, double-shelled particles. Characterization of the poly(A)-containing product of the reaction by gel filtration on Sephadex G-100, followed by chromatography on DEAE-cellulose in the presence of 7 -urea, showed that it is composed of oligonucleotides of a chain length similar to, or slightly larger than, those observed in reovirus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-53-2-357
1981-04-01
2019-10-18
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-53-2-357
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error