Effects of Actinomycin D and Ultraviolet Irradiation on Multiplication of Brome Mosaic Virus in Host and Non-host Cells Free

Abstract

SUMMARY

The modes of multiplication of brome mosaic virus (BMV) were compared in protoplasts isolated from host and non-host plants. BMV actively multiplied in the leaves and isolated mesophyll protoplasts of barley, a host of BMV. BMV multiplication in barley protoplasts was inhibited by addition of actinomycin D immediately after inoculation or by u.v. irradiation of the protoplasts before inoculation. In contrast, although BMV could not multiply in leaves of radish and turnip (non-hosts for BMV) it multiplied at a low level in protoplasts isolated from these two plant species. Moreover, u.v. irradiation, or the addition of actinomycin D, enhanced multiplication of BMV in radish and turnip protoplasts. These results suggest that (i) in the host cells replication of BMV is dependent on cellular metabolism of nucleic acid and protein, and (ii) in the non-host cells a substance(s) inhibitory to replication of BMV is synthesized.

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1981-04-01
2024-03-29
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References

  1. Aoki S., Takebe I. 1969; Infection of tobacco mesophyll protoplasts by tobacco mosaic virus ribonucleic acid. Virology 39:439–448
    [Google Scholar]
  2. Beier H., Siler D. J., Russell M. L., Bruening G. 1977; Survey of susceptibility to cowpea mosaic virus among protoplasts and intact plants from Vigna sinensis lines. Phytopathology 67:917–921
    [Google Scholar]
  3. Fukunaga K., Okuno T., Furusawa I. 1978; Infection of protoplasts isolated from non-host plants with several plant viruses. Annals of the Phytopathological Society of Japan 44:392
    [Google Scholar]
  4. Furusawa I., Okuno T. 1978; Infection with BMV of mesophyll protoplasts isolated from five plant species. Journal of General Virology 40:489–491
    [Google Scholar]
  5. Loebenstein G. 1972; Localization and induced resistance in virus-infected plants. Annual Review of Phytopathology 10:177–206
    [Google Scholar]
  6. Motovoshi F., Oshima N. 1975; Infection with tobacco mosaic virus of leaf mesophyll protoplasts from susceptible and resistant lines of tomato. Journal of General Virology 29:81–91
    [Google Scholar]
  7. Okuno T., Furusawa I. 1978; Modes of infection of barley protoplasts with brome mosaic virus. Journal of General Virology 38:409–418
    [Google Scholar]
  8. Otsuki Y., Shimomura T., Takebe I. 1972; Tobacco mosaic virus multiplication and expression of the N gene in necrotic responding tobacco varieties. Virology 50:45–50
    [Google Scholar]
  9. Rottier P. J. M., Reselman G., Van Kammen A. 1979; The inhibition of cowpea mosaic virus replication by actinomycin D. Virology 92:299–309
    [Google Scholar]
  10. Sakai F., Takebe I. 1974; Protein synthesis in tobacco mesophyll protoplasts induced by tobacco mosaic virus infection. Virology 62:426–433
    [Google Scholar]
  11. Smith S. H., Schlegel D. E. 1965; The incorporation of ribonucleic acid precursors in healthy and virus-infected plant cells. Virology 26:180–189
    [Google Scholar]
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