1887

Abstract

SUMMARY

Recombination between temperature-sensitive () mutants of foot-and-mouth disease (FMD) virus was examined, using an infectious centre technique that was more sensitive (approx. 30-fold) than the conventional virus yield test. The test involved a brief incubation of the mixedly infected cells at the permissive temperature to allow recombination to occur followed by assay at the restrictive temperature to select for those cells in which recombination had occurred. With crosses involving widely separated mutations, as many as 28% of the infected cells produced presumptive recombinant plaques. Since each plaque was the result of an independent event, large numbers of different presumptive recombinants could be isolated for further study. Analysis of presumptive recombinant plaques from a variety of crosses showed that, in general, the virus produced had the properties expected of recombinants. An approximate correlation was found between genetic distance, as determined in the yield recombination test, and the percentage of recombinant infectious centres observed. The phenomenon was very sensitive to the balance between the input multiplicities of the two parent viruses and occurred very early in virus replication. The test has considerable potential for the study of genetic interactions in FMD virus, but it would be surprising if this potential was limited to picornaviruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-53-2-333
1981-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/53/2/JV0530020333.html?itemId=/content/journal/jgv/10.1099/0022-1317-53-2-333&mimeType=html&fmt=ahah

References

  1. Cooper P. D. 1961; An improved agar-cell suspension plaque assay for polio virus: some factors affecting efficiency of plating. Virology 13:153–157
    [Google Scholar]
  2. Cooper P. D. 1968; A genetic map of poliovirus temperature-sensitive mutants. Virology 35:584–596
    [Google Scholar]
  3. Doel T. R., Sangar D. V., Rowlands D. J., Brown F. 1978; A reappraisal of the biochemical map of foot-and-mouth disease virus RNA. Journal of General Virology 41:395–404
    [Google Scholar]
  4. Hirst G. K. 1973; Mechanism of influenza recombination. I. Factors influencing recombination rates between temperature-sensitive mutants of strain WSN and the classification of mutants with complementation-recombination groups. Virology 55:81–93
    [Google Scholar]
  5. King A. M. Q., Newman J. W. I. 1980; Temperature-sensitive mutants of foot-and-mouth disease virus with altered structural polypeptides. I. Identification by electrofocusing. Journal of Virology 34:59–66
    [Google Scholar]
  6. King A. M. Q., Newman J. W. I., Slade W. R., McCahon D. 1980; Temperature-sensitive mutants of foot-and-mouth disease virus with altered structural polypeptides. II. Comparison of recombination and biochemical maps. Journal of Virology 34:67–72
    [Google Scholar]
  7. Lake J. R., Mackenzie J. S. 1973; Improved technique for the isolation of temperature-sensitive mutants of foot-and-mouth disease virus. Journal of Virology 12:665–668
    [Google Scholar]
  8. Lake J. R., Priston R. A. J., Slade W. R. 1975; A genetic recombination map of foot-and-mouth disease virus. Journal of General Virology 27:355–367
    [Google Scholar]
  9. Lowe P. A., Brown F. 1981; Isolation of a soluble and template dependent foot-and-mouth disease virus RNA polymerase. Virology (in press)
    [Google Scholar]
  10. Lowe P. A., King A. M. Q., Newman J. W. I., Slade W. R., McCahon D., Brown F. 1981; Temperature sensitive RNA polymerase mutants of a picornavirus. (in press)
    [Google Scholar]
  11. McCahonm D., Slade W. R., Priston R. A. J., Lake J. R. 1977; An extended genetic recombination map for foot-and-mouth disease virus. Journal of General Virology 35:555–565
    [Google Scholar]
  12. Mackenze J. S., Slade W. R., Lake J. R., Priston R. A. J., Bisby J., Laing S., Newman J. W. I. 1975; Temperature-sensitive mutants of foot-and-mouth disease virus: the isolation of mutants and observations on their properties and genetic recombination. Journal of General Virology 27:61–70
    [Google Scholar]
  13. Macpherson I. A., Stoker M. 1962; Polyoma transformation of hamster cell clones. An investigation of genetic factors affecting cell competence. Virology 16:147–151
    [Google Scholar]
  14. Newman J. F. E., Cartwright B., Doel T. R., Brown F. 1979; Purification and identification of the RNA-dependent RNA polymerase of foot-and-mouth disease virus. Journal of General Virology 45:497–507
    [Google Scholar]
  15. Pfefferkorn E. R. 1977; Genetics of Togaviruses. In Comprehensive Virology vol 9: pp 209–238 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  16. Pringle C. R. 1965; Evidence of genetic recombination in foot-and-mouth disease virus. Virology 25:48–54
    [Google Scholar]
  17. Pringle C. R. 1968; Recombination between conditional-lethal mutants within a strain of foot-and-mouth disease virus. Journal of General Virology 2:199–202
    [Google Scholar]
  18. Sangar D. V., Black D. N., Rowlands D. J., Brown F. 1977; Biochemical mapping of the foot-and-mouth disease virus genome. Journal of General Virology 35:281–297
    [Google Scholar]
  19. Slade W. R., Pringle C. R. 1971; Genetic characteristics of clones from individual cells multiply-infected with different strains of foot-and-mouth disease virus. Journal of General Virology 12:335–339
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-53-2-333
Loading
/content/journal/jgv/10.1099/0022-1317-53-2-333
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error