1887

Abstract

SUMMARY

The properties of polyhedral double-stranded (ds)RNA virus particles obtained from eight isolates of the wheat take-all fungus, var. , have been investigated. Thirteen viruses and virus variants were distinguished and these were classified into three groups on the basis of serological and physical properties of the virus particles; viruses in a group were related serologically to other members of the same group, but unrelated serologically to members of other groups. Group I viruses had particles of diam. 35 nm sedimenting at 109 to 126S; the virus capsid contained one polypeptide species, mol. wt. 54 × 10 to 60 × 10 and virus dsRNA consisted of two to four components, mol. wt. 1.0 × 10 to 1.3 × 10. Group II viruses had particles of diam. 35 nm sedimenting at 133 to 140S; the virus capsid contained one polypeptide species, mol. wt. 68 × 10 to 73 × 10 and virus dsRNA consisted of two to four components with mol. wt. 1.39 × 10 to 1.60 × 10. Group III viruses had particles of diam. 40 nm sedimenting at 159 to 163S; the virus capsid contained three polypeptide species, mol. wt. 78 × 10, 83 × 10 and 87 × 10 and virus dsRNA consisted of two components, mol. wt. 3.2 × 10 to 4.3 × 10.

Five of the fungal isolates contained only single viruses. However, one isolate contained viruses from groups I and II, and two isolates each contained viruses from groups I, II and III. Investigation of virus from ten single conidial isolates derived from one of the latter isolates has shown that all three viruses were present in a single cell.

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1981-04-01
2022-10-04
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References

  1. Bancroft J. B., Mclean G. D., Rees M. W., Short M. N. 1971; The effect of an arginyl to cysteinyl replacement on the uncoating behaviour of a spherical plant virus. Virology 45:707–715
    [Google Scholar]
  2. Banks G. T., Buck K. W., Fleming A. 1971; The isolation of viruses and viral ribonucleic acid from filamentous fungi on a pilot plant scale. Chemical Engineer 251:259–261
    [Google Scholar]
  3. Bostian K. A., Hopper J. E., Rogers D. T., Tipper D. J. 1980; Translational analysis of the killer-associated virus-like particle dsRNA genome of Saccharomyces cerevisiae: M dsRNA encodes toxin. Cell 19:403–414
    [Google Scholar]
  4. Bozarth R. F. 1977; Biophysical and biochemical characterisation of virus-like particles containing high molecular weight dsRNA from Helminthosporium maydis . Virology 80:149–157
    [Google Scholar]
  5. Bozarth R. F., Wood H. A., Mandelbrot A. 1971; The Penicillium stoloniferum virus complex: two similar double-stranded RNA virus-like particles in a single cell. Virology 45:516–523
    [Google Scholar]
  6. Bruenn J., Kane W. 1978; Relatedness of the double-stranded RNAs present in yeast virus-like particles. Journal of Virology 26:762–772
    [Google Scholar]
  7. Buck K. W. 1980; Viruses and killer factors of fungi. In The Eukaryotic Microbial Cell. Society for General Microbiology Symposium 30: pp 329–375 Edited by Gooday G. W., Lloyd D., Trinci A. P. J. Cambridge: Cambridge University Press;
    [Google Scholar]
  8. Buck K. W., Girvan R. F. 1977; Comparison of the biophysical and biochemical properties of Penicillium cyaneo-fulvum virus and Penicillium chrysogenum virus. Journal of General Virology 34:145–154
    [Google Scholar]
  9. Buck K. W., Kempson-Jones G. F. 1973; Biophysical properties of Penicillium stoloniferum virus S. Journal of General Virology 18:223–235
    [Google Scholar]
  10. Buck K. W., Kempson-Jones G. F. 1974; Capsid polypeptides of two viruses isolated from Penicillium stoloniferum . Journal of General Virology 22:441–445
    [Google Scholar]
  11. Buck K. W., Ratti G. 1975; Biophysical and biochemical properties of two viruses isolated from Aspergillus foetidus . Journal of General Virology 27:211–224
    [Google Scholar]
  12. Buck K. W., Ratti G. 1977; Molecular weight of double-stranded RNA: a re-examination of Aspergillus foetidus virus S RNA components. Journal of General Virology 37:215–219
    [Google Scholar]
  13. Deacon J. W. 1976; Biology of the Gaeumannomyces graminis Arx & Olivier/Phialophora radicicola Cain complex on roots of the Gramineae. European Plant Protection Organisation Bulletin 6:349–363
    [Google Scholar]
  14. Frick L. J., Lister R. M. 1978; Serotype variability in virus-like particles from Gaeumannomyces graminis . Virology 85:504–517
    [Google Scholar]
  15. Hirs C. H. W. 1967; Determination of cystine as cysteic acid. Methods in Enzymology 2:59–62
    [Google Scholar]
  16. Hollings M. 1978; Mycoviruses – viruses that infect fungi. Advances in Virus Research 22:3–53
    [Google Scholar]
  17. Huang A. S., Baltimore D. 1977; Defective interfering animal viruses. In Comprehensive Virology vol 10: pp 73–116 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  18. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  19. Lapierre H., Lemaire J.-M., Jouan B., Molin G. 1970; Mise en évidence des particules virales associées à une perte de pathogénicité chez le piétin-échuadage des céréales, Ophiobolus graminis Sacc . Comptes Rendus Hebdomadaires des Séances de l’Académie des Sciences D 271:1833–1836
    [Google Scholar]
  20. Lemaire J. M., Lapierre H., Jouan B., Bertrand G. 1970; Découverte de particules virales chez certaines souche d’Ophiobolus graminis agent du Piétin-échaudage des céréales. Conséquences agronomique prévisibles. Compte Rendu Hebdomadaire des Séances de VAcademie d’Agriculture de France 56:1134–1836
    [Google Scholar]
  21. Rawlinson C., Hornby D., Pearson V., Carpenter J. M. 1973; Virus-like particles in the take-all fungus, Gaeumannomyces graminis . Annals of Applied Biology 74:197–209
    [Google Scholar]
  22. Sehgal O. P., Hsu C. H. 1977; Identity and location of a minor protein component in virions of southern bean mosaic virus. Virology 77:1–11
    [Google Scholar]
  23. Semancik J. S., Vidaver A. K., van Etten J. L. 1973; Characterisation of a segmented double-helical RNA from bacteriophage ϕ 6. Journal of Molecular Biology 78:617–625
    [Google Scholar]
  24. van Etten J. L., Lane L., Gonzalez C., Partridge J. E., Vidaver A. 1976; Comparative properties of bacteriophage ϕ 6 and ϕ 6 nucleocapsid. Journal of Virology 18:652–658
    [Google Scholar]
  25. Weber K., Pringle C. R., Osborn N. 1972; Measurement of molecular weight by electrophoresis on SDS-acrylamide gel. Methods in Enzymology 16:3–27
    [Google Scholar]
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