Isolation and Characterization of Subviral Structures from Tomato Spotted Wilt Virus Free

Abstract

SUMMARY

Particles of a stable isolate of tomato spotted wilt virus (TSWV) had a density of 1.147 g/ml in sucrose and contained four major structural proteins of mol. wt.: 1, 26 × 10; 2, 52 × 10; 3, 56 × 10; and 4, 78 × 10. The RNA which was not infectious consisted of four segments of mol. wt.: 1, 3.6 × 10; 2, 2.8 × 10; 3, 2.0 × 10; and 4, 1.3 × 10. The ribonucleoprotein (RNP) of TSWV, prepared by treatment of purified virus with Nonidet P-40, could be separated into three components by sedimentation on sucrose density-gradients. All three components contained protein 1; the slowest sedimenting component also contained RNA species 1, 2 and 4, the second component contained RNA species 1 and 2 and the fastest sedimenting component contained all four RNA species. The three components were slightly infectious and infectivity was enhanced by pooling these components. The RNP, when observed under the electron microscope, appeared to consist of strands.

Enveloped particles devoid of the outer layer of projections were obtained by treating TSWV with bromelain. These subviral particles retained about 1% of their original infectivity, had a density of 1.137 g/ml in sucrose and only contained protein 1. It was concluded that this protein forms the subunit of the nucleocapsid and that TSWV, unlike the majority of enveloped viruses, does not possess an internal membrane protein.

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1981-04-01
2024-03-29
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References

  1. Brakke M. K., Rochow W. F. 1974; Ribonucleic acid of barley yellow dwarf virus. Virology 61:240–248
    [Google Scholar]
  2. Compans R. W., Content J., Duesberg P. H. 1972; Structure of the ribonucleoprotein of influenza virus. Journal of Virology 10:795–800
    [Google Scholar]
  3. Diener T. O., Schneider I. R. 1968; Virus degradation and nucleic acid release in single phase phenol systems. Archives of Biochemistry and Biophysics 124:401–412
    [Google Scholar]
  4. Fenner F. 1976; Classification and nomenclature of viruses. Intervirology 7:1–115
    [Google Scholar]
  5. Ie T. S. 1970; Tomato spotted wilt virus. CMI/AAB Descriptions of Plant Viruses No. 39, 4 pp
    [Google Scholar]
  6. Lenard J., Compans R. W. 1974; The membrane structure of lipid containing viruses. Biochimica et Biophysica Acta 344:51–94
    [Google Scholar]
  7. Loening U. E. 1968; Molecular weights of ribosomal RNA in relation to evolution. Journal of Molecular Biology 38:355–365
    [Google Scholar]
  8. Mohamed N. A. 1973 Studies on tomato spotted wilt virus Ph.D. thesis University of Adelaide;
    [Google Scholar]
  9. Mohamed N. A. 1978; Physical and chemical properties of cynosurus mottle virus. Journal of General Virology 40:379–389
    [Google Scholar]
  10. Mohamed N. A., Randles J. W., Francki R. I. B. 1973; Protein composition of tomato spotted wilt virus. Virology 56:12–21
    [Google Scholar]
  11. Rifkin D. B., Compans R. W. 1971; Identification of the spike proteins of Rous sarcoma virus. Virology 46:485–489
    [Google Scholar]
  12. Sarkar N. H., Taraschi N. E., Pomenti A. A., Dion A. S. 1976; Polypeptides of the mouse mammary tumour virus. II. Identification of two major glycoproteins with the viral structure. Virology 69:677–690
    [Google Scholar]
  13. Schultze I. T. 1972; The structure of influenza virus. II. A model based on the morphology and composition of subviral particles. Virology 47:181–196
    [Google Scholar]
  14. Tas P. W. L., Boerjan M. L., Peters D. 1977; The structural proteins of tomato spotted wilt virus. Journal of General Virology 36:267–280
    [Google Scholar]
  15. van Den Hurk J., Tas P. W. L., Peters D. 1977; The ribonucleic acid of tomato spotted wilt virus. Journal of General Virology 36:81–91
    [Google Scholar]
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