1887

Abstract

Summary

The type strain of myrobalan latent ringspot virus (MyLRV) and the beet ringspot strain of tomato black ring virus (TBRV-S) were compared with respect to some of their physicochemical and serological properties. A strain of MyLRV without satellite RNA [MyLRV(-3)] and a strain of cherry leafroll virus (CLRV) were also used for comparison purposes. In sucrose density gradients and in the analytical ultracentrifuge, MyLRV and MyLRV(-3) sedimented as three components, T, M and B, with sedimentation coefficients of 50S, 109S and 125S respectively. TBRV-S also had three components sedimenting at 53S (T), 99S (M) and 120S (B).

At equilibrium in CsCl only two components were resolved for TBRV-S with buoyant densities of 1.44 g/ml (M) and 1.50 g/ml (B), whereas MyLRV(-3) yielded three components with the buoyant densities of 1.28 g/ml (T), 1.47 g/ml (M) and 1.51 g/ml (B).

MyLRV preparations contained five additional nucleoproteins (S to S). A study of the distribution of RNAs in the centrifugal components of MyLRV and MyLRV(-3), based on differences in buoyant densities and RNA recovery from different nucleoproteins, suggests that whereas M and B fractions contain one molecule each of RNA-2 and RNA-1, components S to S contain one, two, four and five molecules of satellite RNA-3 respectively. Component S differs from the others in that it may contain one molecule each of RNA-2 and RNA-3. MyLRV and TBRV-S were serologically unrelated. Based on the size of RNA-2, MyLRV appears to be closer to CLRV than to TBRV.

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1981-03-01
2021-10-19
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References

  1. Bishop D. H. L., Claybrook J. R., Spiegelman S. 1967; Electrophoretic separation of viral nucleic acids on polyacrylamide gels. Journal of Molecular Biology 26:373–387
    [Google Scholar]
  2. Boedtker H. 1960; Configurational properties of tobacco mosaic virus ribonucleic acid. Journal of Molecular Biology 2:171–188
    [Google Scholar]
  3. Chervenka C. H. 1969 A Manual of Methods for the Analytical Ultracentrifuge Spinco Division of Beckman Instruments Inc; Palo Alto, California:
    [Google Scholar]
  4. Delbos R., Dunez J., Fisac R. 1976; The RNAs of three strains of tomato black ring virus. Annales de Microbiologie de I’Institute Pasteur 127A:101–109
    [Google Scholar]
  5. Di Franco A., Russo M., Martelli G. P. 1980; Electron microscopy of tomato black ring and myrobalan latent ring spot viruses. Phytopathologia mediterranea 19: (in press)
    [Google Scholar]
  6. Doz B., Dunez J., Delbos R., Bachelier J. C., Monsion M., Macquaire G. 1978; Encapsidation and role of the satellite RNA-3 in different strains of tomato black ring virus. Third International Congress of Plant Pathology, Munchen 41: (abstract)
    [Google Scholar]
  7. Dunez J., Delbos R. 1976; Myrobalan latent ring spot, a bipartite genome virus and a strain of tomato black ring virus. Mitteilungen der Biologischen Bundesanstalt fur Landund Forstwirtschaft 170:23–27
    [Google Scholar]
  8. Dunez J., Delbos R., Desvignes J. C., Marenaud C., Kuszala J., Vuittenez A. 1971; Mise en evidence d’un virus de type ring spot sur Prunus cerasifera. Annales de Phytopathologie Hors Serie117–128
    [Google Scholar]
  9. Dunn D. B., Hitchborn J. H. 1965; The use of bentonite in the purification of plant viruses. Virology 25:171–192
    [Google Scholar]
  10. Harrison B. D. 1958; Further studies on raspberry ring spot and tomato black ring, soil borne viruses that affect raspberry. Annals of Applied Biology 46:571–584
    [Google Scholar]
  11. Harrison B. D., Murant A. F. 1977; Nepovirus group. Commonwealth Mycological Institute/Association of Applied Biologists. Descriptions of Plant Viruses No 185:
    [Google Scholar]
  12. Jones A. T., Mayo M. A. 1972; The two nucleoprotein particles of cherry leaf roll virus. Journal of General Virology 16:349–358
    [Google Scholar]
  13. Jones A. T., Murant A. F. 1971; Serological relationship between cherry leaf roll, elm mosaic and golden elderberry viruses. Annals of Applied Biology 69:11–15
    [Google Scholar]
  14. Kaper J. M., Waterworth H. E. 1973; Comparison of molecular weights of single stranded viral RNAs by two empirical methods. Virology 51:183–190
    [Google Scholar]
  15. Martelli G. P., Quacquarelli A., Gallitelli D., Savino V., Piazzolla P. 1978; A tentative grouping of nepoviruscs. Phytopathologia mediterranea 17:145–147
    [Google Scholar]
  16. Murant A. F., Taylor M. 1978; Estimates of molecular weights of nepovirus RNA species by polyacrylamide gel electrophoresis under denaturing conditions. Journal of General Virology 41:53–61
    [Google Scholar]
  17. Murant A. F., Mayo M. A., Harrison B. D., Goold R. A. 1973; Evidence for two functional RNA species and a ‘satellite’ RNA in tomato black ring virus. Journal of General Virology 19:275–278
    [Google Scholar]
  18. Quacquarelli A., Gallitelli D., Savino V., Piazzolla P., Martelli G. P. 1976a; Some properties of grapevine fanleaf and other nepoviruses infecting the grapevine. VI Conferencia Internacional sobre virus y virosis de la Vid41–51
    [Google Scholar]
  19. Quacquarelli A., Gallitelli D., Savino V., Martelli G. P. 1976b; Properties of grapevine fanleaf virus. Journal of General Virology 32:349–360
    [Google Scholar]
  20. Raymer W. B., Diener T. O. 1969; Potato spindle tuber virus: a plant virus with properties of a free nucleic acid. 1. Assay, extraction and concentration. Virology 37:343–350
    [Google Scholar]
  21. Stanley W. M., Bock R. M. 1965; Isolation and physical properties of the ribosomal ribonucleic acid of Escherichia coli. Biochemistry 4:1302–1304
    [Google Scholar]
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