Purification and Characterization of Ovine Astrovirus Free

Abstract

Summary

Astrovirus, purified in aggregated form from epithelial cells of the small intestinal villi of infected gnotobiotic lambs, was shown to have a single-stranded RNA genome with an S value of 34 and poly(A) tract. Only two major capsid polypeptides were detected with similar mol. wt. of approx. 3.3 × 10.

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1981-03-01
2024-03-28
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References

  1. Bachrach H. L., Hess W. R. 1973; Animal picornaviruses with a single major species of capsid protein. Biochemical and Biophysical Research Communications 55:141–149
    [Google Scholar]
  2. Bevan E. A., Herring A. J., Mitchell D. J. 1973; Two species of dsRNA in yeast: their preliminary characterisation and relationship to the killer character. Nature, London 245:81–86
    [Google Scholar]
  3. Bishop J. O., Rosbash M., Evans D. 1974; Polynucleotide sequences in eukaryotic DNA and RNA that form ribonuclease-resistant complexes with polyuridylic acid. Journal of Molecular Biology 85:75–86
    [Google Scholar]
  4. Burroughs J. N., Rown F. 1974; Physico-chemical evidence for the reclassification of the caliciviruses. Journal of General Virology 22:281–286
    [Google Scholar]
  5. Cooper V. I., Agol P. D., Bachrach H. L., Brown F., Ghendon Y., Gibbs A. J., Gillespie J. H., Lonberg-Holm K., Mandel B., Melnick J. L., Moharty S. B., Povey R. C., Rueckert R. R., Schaffer F. L., Tyrrell D. A. J. 1978; Picornaviridae: second report. Intervirology 10:165–180
    [Google Scholar]
  6. Emtage J. S., Carey N. H., Stebbing N. 1976; Structural features of encephalomyocarditis virus RNA from analysis of reverse transcription products. European Journal of Biochemistry 69:69–78
    [Google Scholar]
  7. Galdiero F. 1979; Adenovirus aggregation and preservation in extracellular environment. Archives of Virology 59:99–105
    [Google Scholar]
  8. Giron M., Logeat F., Hamania N., Fossar N., Huppert J. 1976; Size of the poly-A sequences in encephalomyocarditis virus RNA. Intervirology 6:367–371
    [Google Scholar]
  9. Gray E. W., Angus K. W., Snodgrass D. R. 1980; Ultrastructure of the small intestine in astrovirus infected lambs. Journal of General Virology 49:71–82
    [Google Scholar]
  10. Kerr I. M., Martin E. M. 1972; Simple method for the isolation of encephalomyocarditis virus ribonucleic acid. Journal of Virology 9:559–561
    [Google Scholar]
  11. Kurtz J. B., Lee T. W., Craig J. W., Reed S. E. 1979; Astrovirus infections in volunteers. Journal of Medical Virology 3:221–230
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 121:680–685
    [Google Scholar]
  13. Lee T. W., Kurtz J. B. 1977; Astroviruses detected by immunofluorescence. Lancet 11:406
    [Google Scholar]
  14. Loening U. E. 1967; The fractionation of high molecular weight RNA by polyacrylamide electrophoresis. Biochemical Journal 102:251–257
    [Google Scholar]
  15. Madeley C. R., Cosgrove B. P. 1975; Viruses in infantile gastroenteritis. Lancet 11:451–452
    [Google Scholar]
  16. Martin R. G., Ames B. N. 1961; A method for determining the sedimentation behaviour of enzymes: application to protein mixtures. Journal of Biological Chemistry 236:1372–1379
    [Google Scholar]
  17. Newman J. F. E., Rowlands D. J., Brown F. 1973; A physico-chemical sub-grouping of the mammalian picornaviruses. Journal of General Virology 18:171–180
    [Google Scholar]
  18. Oglesby A. S., Schaffer F. L., Martin S. H. 1971; Biochemical and biophysical properties of vesicular exanthema of swine virus. Virology 44:329–341
    [Google Scholar]
  19. Parish J. H., Kirby K. S. 1966; Reagents which reduce interactions between ribosomal RNA and rapidly labelled RNA from rat liver. Biochimica et Biophysica Acta 129:544–562
    [Google Scholar]
  20. Snodgrass D. R., Gray E. W. 1977; Detection and transmission of 30 nm virus particles (astroviruses) in faeces of lambs with diarrhoea. Archives of Virology 55:287–291
    [Google Scholar]
  21. Snodgrass D. R., Angus K. W., Gray E. W., Menzies J. D., Paul G. 1979; Pathogenesis of diarrhoea caused by astrovirus infections in lambs. Archives of Virology 60:217–226
    [Google Scholar]
  22. Talbot P., Rowlands D. J., Burroughs J. N., Sangar D. V., Brown F. 1973; Evidence for a group protein in foot-and-mouth disease virus particles. Journal of General Virology 19:369–380
    [Google Scholar]
  23. Todd D., McNulty M. S. 1976; Characterization of pig rotavirus RNA. Journal of General Virology 33:147–150
    [Google Scholar]
  24. Weber K., Osborn M. 1969; The reliability of molecular weight determinations by dodecyl sulphate polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  25. Williams D. L., Klett H. 1978; Measurement of polyadenylic acid by hybridisation with polyuridylic acid: a source of error due to the lability of tritiated polyuridylic acid in trichloroacetic acid. Analytical Biochemistry 91:173–179
    [Google Scholar]
  26. Woode G. N., Bridger J. C. 1978; Isolation of small viruses resembling astroviruses and caliciviruses from acute enteritis of calves. Journal of Medical Microbiology 11:441–452
    [Google Scholar]
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