1887

Abstract

Summary

Considerable differences exist in the lethality of herpes simplex virus type 1 (HSV-1) after intraperitoneal (i.p.) infection in different inbred strains of mice. In this study humoral and cellular immunity and interferon production were compared in resistant and susceptible mice. The serum IgG response, as determined by an enzyme-linked immunosorbent assay (ELISA), was the same in different strains of mice. There was also no difference in neutralizing antibodies between resistant C57BL/6 and susceptible DBA/2 mice. The production of macrophage migration inhibitory factor, obtained from spleen cells of mice 7 days after infection with different doses of HSV-1, was the same in resistant and susceptible mice. Serum interferon could be detected 8 h after i.p. injection of 10 p.f.u. of HSV-1, but not at lower virus doses. At 8 h, high interferon titres [>1000 reference research units (iu)/ml] were observed in the serum of C57BL/6 mice but low titres (about 100 iu/ml) were found in the serum of DBA/2 mice. At 24 h, the titres were low in both strains of mice. Interferon production was also measured in spleen cell cultures exposed to inactivated HSV-1. These studies also showed high interferon production in spleen cell cultures of resistant mice, whereas low titres were produced by spleen cells of susceptible mice. Thus, our study has failed to reveal any differences in humoral or cellular immunity in mice resistant or susceptible to HSV but a difference in HSV-induced interferon production.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-53-1-31
1981-03-01
2022-01-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/53/1/JV0530010031.html?itemId=/content/journal/jgv/10.1099/0022-1317-53-1-31&mimeType=html&fmt=ahah

References

  1. Andervont H. B. 1929; Activity of herpetic virus in mice. Journal of Infectious Diseases 44:383–392
    [Google Scholar]
  2. Bang F. B. 1978; Genetics of resistances of animals to viruses. I. Introduction and studies in mice. Advances in Virus Research 23:269–347
    [Google Scholar]
  3. Bang F. B., Warwick A. 1960; Mouse macrophages as host cells for the mouse hepatitis virus and the genetic basis of their susceptibility. Proceedings of the National Academy of Sciences of the United States of America 46:1065–1075
    [Google Scholar]
  4. Bidwell D. E., Bartlett A., Voller A. 1977; Enzyme immunoassays for viral diseases. Journal of Infectious Diseases 136:274–278
    [Google Scholar]
  5. Burns W. H., Billups L. C., Notkins A. L. 1975; Thymic dependence of viral antigens. Nature, London 256:654–656
    [Google Scholar]
  6. DeMaeyer E., DeMaeyer-Guignard J. 1969; Gene with quantitative effect on circulating interferon induced by Newcastle disease virus. Journal of Virology 3:506–512
    [Google Scholar]
  7. DeMaeyer E., DeMayer Guignard J., Hall W. T., Bailey D. W. 1974; A locus affecting circulating interferon levels induced by mouse mammary tumour virus. Journal of General Virology 23:209–211
    [Google Scholar]
  8. Haahr S., Rasmussen L., Merigan T. C. 1976; Lymphocyte transformation and interferon production in human mononuclear cell microcultures for assay of cellular immunity to herpes simplex virus. Infection and Immunity 14:47–54
    [Google Scholar]
  9. Kirchner H., Hirt H. M., Munk K. 1977; Protection against herpes simplex virus infection in mice by Corynebacterium parvum. Infection and Immunity 16:9–11
    [Google Scholar]
  10. Kirchner H., Kochen M., Hirt H. M., Munk K. 1978a; Immunological studies of HSV infection of resistant and susceptible inbred strains of mice. Zeitschrift für Immunitatsforschung 154:147–154
    [Google Scholar]
  11. Kirchner H., Zawatzky R., Hirt H. M. 1978b; In vitro production of immune interferon by spleen cells of mice immunized with herpes simplex virus. Cellular Immunology 40:204–210
    [Google Scholar]
  12. Kirchner H., Keyssner K., Zawatzky R., Hilfenhaus J. 1980; Studies of the producer cell of herpes simplex virus-induced interferon in mouse spleen cell cultures. Immunobiology (in press)
    [Google Scholar]
  13. Landolfo S., Marcucci F., Giovarelli M., Viano I., Forni G. 1979; Lymphokine production in mouse mixed lymphocyte reaction (MLR). II. Tentative mapping of murine alloantigens activating migration inhibition factor and interferon release and their relationship with those activating proliferative response. Immunogenetics 9:245–253
    [Google Scholar]
  14. Lopez C. 1975; Genetics of natural resistance to herpesvirus infections in mice. Nature, London 258:152–153
    [Google Scholar]
  15. Lopez C. 1978; Immunoldgical nature of genetic resistance of mice to herpes simplex virus type-1 infection. In Oncogenesis and Herpesviruses vol 3: Edited by de The G., Henle W., Rapp F. IARC Scientific Publications, 24;
    [Google Scholar]
  16. Lopez C. 1980; Resistance to HSV-1 in the mouse is governed by two major independently segregating, non-H-2 loci. Immunogenetics (in press)
    [Google Scholar]
  17. Lopez C., Ryshke R., Bennett M. 1980; Marrow-dependent cells depleted by 89Sr mediate genetic resistance to herpes simplex virus type 1 infection in mice. Infection and Immunity 28:1028–1032
    [Google Scholar]
  18. Morahan P. S., Breinig M. C., McGeorge M. B. 1977; Immune response to vaginal or systemic infection of BALB/c mice with herpes simplex virus type 2. Journal of Immunology 119:2030–2036
    [Google Scholar]
  19. Notkins A. L. 1974; Commentary: immune mechanisms by which the spread of viral infection is stopped. Cellular Immunology 11:478–483
    [Google Scholar]
  20. Pfizenmaier K., Jung H., Starzinzki-Powitz A., Röllinghoff M., Wagner H. 1977; The role of T cells in anti-herpes simplex virus immunity. I. Induction of antigen-specific cytotoxic T lymphocytes. Journal of Immunology 119:939–944
    [Google Scholar]
  21. Sethi K. K., Brandis H. 1977; Specifically immune mouse T cells can destroy H-2 compatible murine target cells infected with herpes simplex virus types 1 or 2. Zeitschrift für Immunitätsforschung 150:162–173
    [Google Scholar]
  22. Stevens J. C., Cook M. L. 1971; Restriction of herpes simplex virus by macrophages. An analysis of cell-virus interactions. Journal of Experimental Medicine 133:19–38
    [Google Scholar]
  23. Zawatzky R., Hilfenhaus J., Kirchner H. 1979; Resistance of nude mice to herpes simplex virus and correlation with in vitro production of interferon. Cellular Immunology 47:424–428
    [Google Scholar]
  24. Zisman B., Hirsch M. S., Allison A. C. 1970; Selective effects of anti-macrophage serum, silica and anti-lymphocyte serum in pathogenesis of herpes virus infection of young adult mice. Journal of Immunology 104:1155–1159
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-53-1-31
Loading
/content/journal/jgv/10.1099/0022-1317-53-1-31
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error