1887

Abstract

Summary

Purified preparations of a closterovirus isolated from a grapevine with stem-pitting symptoms (GSP-AV) had u.v. absorption spectra typical of a nucleoprotein with low nucleic acid content. GSP-AV coat protein had a mol. wt. of about 2.2 × 10. Its nucleic acid was identified as a single-stranded RNA on the basis of (i) base composition (U, 22.3; C, 24.9; A, 24.4; G, 28.4 mol%), (ii) sensitivity to pancreatic ribonuclease A and (iii) melting behaviour ( at about 52 °C in 0.15 -NaCl-0.015 -sodium citrate). In 2.4% aqueous polyacrylamide gels, GSP-AV RNA had an apparent mol. wt. of 2.55 × 10.

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1981-03-01
2021-10-23
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