1887

Abstract

Summary

Purified preparations of a closterovirus isolated from a grapevine with stem-pitting symptoms (GSP-AV) had u.v. absorption spectra typical of a nucleoprotein with low nucleic acid content. GSP-AV coat protein had a mol. wt. of about 2.2 × 10. Its nucleic acid was identified as a single-stranded RNA on the basis of (i) base composition (U, 22.3; C, 24.9; A, 24.4; G, 28.4 mol%), (ii) sensitivity to pancreatic ribonuclease A and (iii) melting behaviour ( at about 52 °C in 0.15 -NaCl-0.015 -sodium citrate). In 2.4% aqueous polyacrylamide gels, GSP-AV RNA had an apparent mol. wt. of 2.55 × 10.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-53-1-179
1981-03-01
2019-10-21
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-53-1-179
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error