1887

Abstract

Summary

The haemagglutinin chains HA1 and HA2 from the avian influenza virus A/duck/Ukraine/1/63 (Hav7, Neq2) have been subjected to amino acid analysis and N-terminal sequencing. Automated sequenator analysis of HA1 (40 cycles), after enzymic removal of the N-terminal pyroglutamic acid blocking group, and HA2 (43 cycles) showed that the Hav7 haemagglutinin closely resembled the human Hong Kong (H3) haemagglutinins including the presence of the characteristic extended 10 residue sequence at the N-terminus of HA1. These findings, together with the amino acid compositions for both chains, demonstrate that the Hav7 haemagglutinin is structurally similar to the Hong Kong (H3) haemagglutinins.

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1981-03-01
2022-12-04
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References

  1. Air G. M. 1980; Sequences from the 3′ ends of influenza virus RNA segments. In Structure and Variation in Influenza Virus pp 135–146 Edited by Laver G., Air G. New York: Elsevier;
    [Google Scholar]
  2. Bean W. J., Cox N. J., Kendal A. P. 1980; Recombination of human influenza A viruses in nature. Nature, London 284:638–640
    [Google Scholar]
  3. Both G. W., Sleigh M. J., Bender V. J., Moss B. A. 1980; A comparison of antigenic variation in Hong Kong influenza virus haemagglutinins at the nucleic acid level. In Structure and Variation in Influenza Virus pp 81–89 Edited by Laver G., Air G. New York: Elsevier;
    [Google Scholar]
  4. Bucher D. 1977; Identification of subnanomole amounts of PTH-amino acids by high performance thin-layer chromatography. Chromalographia 10:723–725
    [Google Scholar]
  5. Bucher D. J., Li S. S. L., Kehoe J. M., Kilbourne E. D. 1976; Chromatographic isolation of the haemagglutinin polypeptides from influenza virus vaccine and determination of their amino-terminal sequences. Proceedings of the National Academy of Sciences of the United States of America 73:238–242
    [Google Scholar]
  6. Coleman M. T., Dowdle W. R., Pereira H. G., Schild G. C., Chang W. K. 1968; The Hong Kong influenza A2 variant. Lancet ii:1384–1386
    [Google Scholar]
  7. Dopheide T. A., Ward C. W. 1978; The carboxyl-terminal sequence of the heavy chain of a Hong Kong influenza haemagglutinin. European Journal of Biochemistry 85:393–398
    [Google Scholar]
  8. Dopheide T. A., Ward C. W. 1979; A Hong Kong influenza hemagglutinin light chain: the amino acid sequence of cyanogen bromide fragments CN3 and CN4 and the N-terminal 45 residues. Virology 92:230–235
    [Google Scholar]
  9. Dopheide T. A., Ward C. W. 1980; The amino acid sequence of a Hong Kong influenza haemagglutinin light (HA2) chain. Journal of General Virology 50:329–335
    [Google Scholar]
  10. Fazekas De St, Groth S. 1970; Evolution and hierarchy of influenza viruses. Archives of Environmental Health 21:293–303
    [Google Scholar]
  11. Fazekas De St, Groth S. 1978; Antigenic, adaptive and absorptive variants of influenza A hemagglutinin. In The Influenza Virus Hemagglutinin pp 25–48 Edited by Laver W. G., Bachmayer H., Weil R. Vienna: Springer-Verlag;
    [Google Scholar]
  12. Gething M. J., Bye J., Skehel J. J., Waterfield M. D. 1980; Cloning and DNA sequence of double-stranded copies of hemagglutinin genes from H2 and H3 strains of influenza virus. In Structure and Variation in Influenza Virus pp 1–10 Edited by Laver G., Air G. New York: Elsevier;
    [Google Scholar]
  13. Hinshaw V. S., Bean W. J., Webster R. G., Sriram G. 1980; Genetic reassortment of influenza A viruses in the intestinal tract of ducks. Virology 102:412–419
    [Google Scholar]
  14. Inglis A. S., Strike P. M., Osborne W. C., Burley R. W. 1979; Sequenator determination of the amino acid sequence of apovitellenin I from turkey’s egg yolk. FEBS Letters 97:179–182
    [Google Scholar]
  15. Kaplan M. M., Webster R. G. 1978; The epidemiology of influenza. Scientific American 237:88–106
    [Google Scholar]
  16. Laver W. G. 1969; Purification of influenza virus. In Fundamental Techniques in Virology pp 82–86 Edited by Habel K., Salzman N. P. New York: Academic Press;
    [Google Scholar]
  17. Laver W. G. 1971; Separation of the two polypeptide chains from the hemagglutinin subunit of influenza virus. Virology 45:275–288
    [Google Scholar]
  18. Laver W. G., Webster R. G. 1973; Studies on the origin of pandemic influenza. III. Evidence implicating duck and equine influenza viruses as possible progenitors of the Hong Kong strain of human influenza. Virology 51:383–391
    [Google Scholar]
  19. Laver W. G., Webster R. G. 1977; Hemagglutinin molecules of Hong Kong, equine-2 and duck/Ukraine influenza viruses lack N-terminal aspartic acid. Virology 81:482–485
    [Google Scholar]
  20. Laver W. G., Webster R. G. 1979; Ecology of influenza viruses in lower mammals and birds. British Medical Bulletin 35:29–33
    [Google Scholar]
  21. Laver W. G., Air G. M., Dopheide T. A., Ward C. W. 1980; Amino acid sequence changes in the haemagglutinin of A/Hong Kong (H3N2) influenza virus during the period 1968-1977. Nature, London 283:454–457
    [Google Scholar]
  22. Masurel N. 1969; Relation between Hong Kong virus and former human A2 isolates and the A/Equi 2 virus in human sera collected before 1957. Lancet i:907–910
    [Google Scholar]
  23. Min-Jou W., Verhoeyen M., Devos R., Saman E., Huylebroeck D., Van Rompuy L., Fang R. X., Fiers W. 1980; Cloning and DNA nucleotide sequence analysis of the hemagglutinin and neuraminidase genes of influenza A-strains. In Structure and Variation in Influenza Virus pp 63–68 Edited by Laver G., Air G. New York: Elsevier;
    [Google Scholar]
  24. Moore S. 1963; On the determination of cystine as cysteic acid. Journal of Biological Chemistry 238:235–237
    [Google Scholar]
  25. Porter A. G., Barber C., Carey N. H., Hallewell R. A., Threlfall G., Emtage J. S. 1979; Complete nucleotide sequence of an influenza virus haemagglutinin gene from cloned DNA. Nature, London 282:471–477
    [Google Scholar]
  26. Schild G. C., Newman R. W., Webster R. G., Major D., Hinshaw V. 1980; Antigenic analysis of the hemagglutinin, neuraminidase and nucleoprotein antigens of influenza A viruses. In Structure and Variation in Influenza Virus pp 373–383 Edited by Laver G., Air G. New York: Elsevier;
    [Google Scholar]
  27. Scholtissek C., Rohde W., Von Hoyningen V., Rott R. 1978; On the origin of the human influenza virus sub-types H2N2 and H3N2. Virology 87:13–20
    [Google Scholar]
  28. Simpson R. J., Neuberger M. R., Liu T-Y. 1976; Complete amino acid analysis of proteins from a single hydrolysate. Journal of Biological Chemistry 251:1936–1940
    [Google Scholar]
  29. Skehel J. J., Waterfield M. D. 1975; Studies on the primary structure of the influenza virus hemagglutinin. Proceedings of the National Academy of Sciences of the United States of America 72:93–97
    [Google Scholar]
  30. Smithies O., Gibson D., Fanning E. M., Goodfliesh R. M., Gilman J. G., Ballantyne D. L. 1971; Quantitative procedures for use with the Edman-Begg sequenator. Partial sequences of two unusual immunoglobulin light chains, Rzf and Sac. Biochemistry 10:4912–4921
    [Google Scholar]
  31. Tumova B., Easterday B. C. 1969; Relationship of envelope antigens of animal influenza viruses to human A2 influenza strains isolated in the years 1957-1968. Bulletin of the World Health Organization 41:429–435
    [Google Scholar]
  32. Ward C. W., Dopheide T. A. 1980a; Completion of the amino acid sequence of a Hong Kong influenza hemagglutinin heavy chain: sequence of cyanogen bromide fragment CN1. Virology 103:37–53
    [Google Scholar]
  33. Ward C. W., Dopheide T. A. 1980b; Amino acid sequence of the hemagglutinin from an early Hong Kong variant X-31. Biochemical Journal (in press)
    [Google Scholar]
  34. Waterfield M. D., Espelie K., Elder K., Skehel J. J. 1979; Structure of the hemagglutinin of influenza virus. British Medical Bulletin 35:57–63
    [Google Scholar]
  35. Webster R. G. 1970; Antigenic hybrids of influenza A viruses with surface antigens to order. Virology 42:633–642
    [Google Scholar]
  36. Webster R. G., Laver W. G. 1975; Antigenic variation in influenza viruses. In The Influenza Viruses and Influenza pp 269–314 Edited by Kiibourne E. D. New York: Academic Press;
    [Google Scholar]
  37. Young J. F., Palese P. 1979; Evolution of human influenza A viruses in nature: recombination contributes to genetic variation of H1N1 strains. Proceedings of the National Academy of Sciences of the United States of America 76:6547–6551
    [Google Scholar]
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