1887

Abstract

Summary

We report data on the number of Epstein-Barr virus (EBV) particles required to detect virus binding to target cells (Raji or BJA-B) by means of membrane immunofluorescence (MIF). After determining the optimum conditions for the MIF assay the following aspects of EBV—lymphoid cell interactions were examined: (i) binding of two different strains of EBV to various types of human lymphoid cell lines, (ii) expression of receptors for both EBV and complement on these lines and (iii) induction of EBV-induced nuclear antigen (EBNA) in the different target cells used. The results showed that a minimum of about 2.7 × 10 enveloped virus particles/cell were required for an optimum visualization of EBV binding to the target cells tested, and that a lymphoid cell may bear receptors for one prototype strain of EBV but not for the other. A number of cell lines, particularly those of T and null type which express EBV receptors, did not synthesize EBNA, thus indicating that these lines were resistant to EBV infection. Several of these lines, although expressing cell surface EBV receptors, lacked complement receptors.

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1981-03-01
2024-12-07
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References

  1. Adams R. A., Pothier A., Flowers A., Lazarus H., Farber S., Foley G. 1970; The question of Stemlines in human acute leukemia. Comparison of cells isolated in vitro and in vivo from a patient with acute lymphoblastic leukemia. Experimental Cell Research 62:5–10
    [Google Scholar]
  2. Aiuti F., Cerottini J. C., Coombs R. R. A. 1975; International Union of Immunological Societies (IUIS) Report, July 1974. Identification, enumeration and isolation of B and T lymphocytes from human peripheral blood. Clinical Immunology and Immunopathology 3:584–597
    [Google Scholar]
  3. Einhorn L., Steinitz M., Yefenof E., Ernberg I., Bakaks T., Klein G. 1978; Epstein-Barr virus (EBV) receptors, complement receptors and EBV infectibility of different lymphocyte fractions of human peripheral blood. II. Epstein-Barr virus studies. Cellular Immunology 35:43–58
    [Google Scholar]
  4. Foley G. E., Lazarus H., Farber S., Uzman B. G., Boone B. A., Maccarty R. E. 1965; Continuous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer 18:522–529
    [Google Scholar]
  5. Glaser R., Lenoir G., Ferrone S., Pellegrino M. A., De Thé G. 1977; Cell surface markers on epithelial-Burkitt hybrid cells superinfected with Epstein-Barr virus. Cancer Research 37:2291–2296
    [Google Scholar]
  6. Greaves M. F., Brown G., Rickinson A. B. 1975; Epstein-Barr virus binding sites on lymphocyte subpopulations and the origin of lymphoblasts in cultured lymphoid cell lines and in the blood of patients with infectious mononucleosis. Clinical Immunology and Immunopathology 3:514–524
    [Google Scholar]
  7. Haider S., Coutinho M. D., Emond R. T. D. 1973; Tuberculin anergy and infectious mononucleosis. Lancet ii:74
    [Google Scholar]
  8. Henle W., Henle G. 1972; Epstein-Barr virus: the cause of infectious mononucleosis: a review. In Oncogenesis and Herpesviruses pp 187–209 Edited by Biggs P. M., de The G., Payne L. N. Lyon: IARC Scientific Publications;
    [Google Scholar]
  9. Hinuma Y., Grace J. T. 1967; Cloning of immunoglobulin-producing human leukemic and lymphoma cells in long term cultures. Proceedings of the Society for Experimental Biology and Medicine 124:107–111
    [Google Scholar]
  10. Huang C. C., Hou Y., Woods L. K., Moore G. E., Minowada J. 1974; Genetic study of human lymphoid T-cell lines derived from lymphocytic leukemia. Journal of the National Cancer Institute 53:655–660
    [Google Scholar]
  11. Jondal M., Klein G. 1973; Surface markers on human B and T lymphocytes. II. Presence of Epstein-Barr virus receptors on B lymphocytes. Journal of Experimental Medicine 138:1365–1378
    [Google Scholar]
  12. Jondal M., Klein G., Oldstone M. B. A., Bokish V., Yefenof E. 1976; Surface markers on human B and T lymphocytes. VIII. Association between complement and Epstein-Barr virus receptors on human lymphoid cells. Scandinavian Journal of Immunology 5:401–410
    [Google Scholar]
  13. Katsuki T., Hinuma Y., Yamamoto N., Abo T., Kumagai K. 1977; Identification of the target cells in human B lymphocytes for transformation by Epstein-Barr virus. Virology 83:287–294
    [Google Scholar]
  14. Klein G., Lindahl T., Jondal M., Leibold W., Menezes J., Nilsson K., Sundstrӧm C. 1974; Continuous lymphoid cell lines with characteristics of B cells (bone-marrow-derived), lacking the Epstein-Barr virus genome and derived from three human lymphomas. Proceedings of the National Academy of Sciences of the United States of America 71:3283–3286
    [Google Scholar]
  15. Klein G., Manneborg A., Steinitz M. 1979; Differences in EBV receptor concentration between in vitro EBV-converted lymphoma sublines reflect biological differences between the converting viral substrains. International Journal of Cancer 23:197–200
    [Google Scholar]
  16. Lonberg Holm K., Philipson L. 1974; Early interaction between animal viruses and cells. Monographs in Virology 9: (in press)
    [Google Scholar]
  17. Lozzio C. B., Lozzio B. B. 1973; Cytotoxicity of a factor isolated from human spleen. Journal of the National Cancer Institute 50:535–538
    [Google Scholar]
  18. Mangi R. J., Niederman J. C., Kellehey J. E., Dwyer I. M., Evans A. S., Kantor F. S. 1974; Depression of cell-mediated immunity during acute infectious mononucleosis. New England Journal of Medicine 291:1149–1153
    [Google Scholar]
  19. Menezes J., Leibold W., Klein G. 1975a; Biological differences between Epstein-Barr virus strains with regard to lymphocyte transforming ability, superinfection and antigen induction. Experimental Cell Research 93:478–484
    [Google Scholar]
  20. Menezes J., Leibold W., Klein G., Clements G. 1975b; Establishment and characterization of an Epstein-Barr virus (EBV) negative lymphoblastoid B cell line from an exceptional, EBV-genome negative African Burkitt’s lymphoma. Biomedicine 22:276–284
    [Google Scholar]
  21. Menezes J., Jondal M., Leibold W., Dorval G. 1976; Epstein-Barr virus interactions with human lymphocyte subpopulations: virus adsorption, kinetics of expression of Epstein-Barr virus associated nuclear antigen, and lymphocyte transformation. Infection and Immunity 13:303–310
    [Google Scholar]
  22. Menezes J., Seigneurin J. M., Patel P., Bourkas A., Lenoir G. 1977; Presence of Epstein-Barr virus receptors, but absence of virus penetration in cells of an Epstein-Barr virus genome-negative human lymphoblastoid T line (Molt-4). Journal of Virology 22:816–821
    [Google Scholar]
  23. Menezes J., Patel P., Bourkas A. E. 1978a; Interaction of Epstein-Barr virus (EBV) with EBV-genome-negative, receptor-positive human lymphoid cell lines (LCL) derived from acute lymphoblastic leukemia (ALL) and lymphoma patients. In Advances in Comparative Leukemia Research 1977: pp 216–218 Edited by Bentvelzen P., Hilgers J., Yohn D. S. Amsterdam: Elsevier/North-Holland Biomedical Press;
    [Google Scholar]
  24. Menezes J., Patel P., Dussault M., Bourkas A. E. 1978b; Comparative studies on the induction of virus-associated nuclear antigen and early antigen by lymphocyte-transforming (B95-8) and nontransforming (P3HR-1) strains of Epstein-Barr virus. Intervirology 9:86–94
    [Google Scholar]
  25. Minowada J., Klein G., Clifford P., Klein E., Moore G. E. 1967; Studies of Burkitt lymphoma cells. I. Establishment of a cell line (B35M) and its characteristics. Cancer 20:1430–1437
    [Google Scholar]
  26. Minowada J., Ohnuma T., Moore G. E. 1972; Rosette forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus derived lymphocytes. Journal of the National Cancer Institute 49:891–895
    [Google Scholar]
  27. Minowada J., Janossy G., Greaves M. F., Tsubota T., Srivastava B. I. S., Morikawa S., Tatsumi E. 1978; Expression of an antigen associated with acute lymphoblastic leukemia in human leukemia-lymphoma cell lines. Journal of the National Cancer Institute 60:1269–1277
    [Google Scholar]
  28. Miyoshi I., Hiraki S., Tsubota T., Kubonishi I., Matsuda Y., Nakayama T., Kishimoto H., Kimura I., Masuji H. 1977; Human B cell, T cell and null cell leukaemic cell lines derived from acute lymphoblastic leukaemias. Nature, London 267:843–844
    [Google Scholar]
  29. Monroe J. M., Brandt P. M. 1970; Rapid semiquantitative method for screening large numbers of virus samples by negative staining electron microscopy. Applied Microbiology 20:259–262
    [Google Scholar]
  30. Nilsson K., Sundstrӧm C. 1974; Establishment and characteristics of two unique cell lines from patients with lymphosarcoma. International Journal of Cancer 13:808–823
    [Google Scholar]
  31. Patel P. C., Menezes J., Bourkas A., Dorval G., Rivard G. E., Boulay G., Stanley P., Joncas J. H. 1978; Rosette formation by human lymphoid cells with monkey red blood cells. Annales d’Immunology 129c:449–462
    [Google Scholar]
  32. Pulvertaft R. J. V. 1965; A study of malignant tumors in Nigeria by short term tissue culture. Journal of Clinical Pathology 18:261–273
    [Google Scholar]
  33. Robb J. A., Martin R. G. 1972; Genetic analysis of simian virus 40. III. Characterization of a temperature-sensitive mutant blocked at an early stage of productive infection in monkey cells. Journal of Virology 9:956–968
    [Google Scholar]
  34. Robinson J. E., Andiman W. A., Henderson E., Miller G. 1977; Host-determined differences in expression of surface marker characteristics on human and simian lymphoblastoid cell lines transformed by Epstein-Barr virus. Proceedings of the National Academy of Sciences of the United States of America 74:749–753
    [Google Scholar]
  35. Rosenfeld C., Goutner A., Venuat A. M., Choquet C., Pico J. L., Dore J. F., Liabeuf A., Durandy A., Desgranges C., De Thé G. 1977; An effective human leukemic cell line: Reh. European Journal of Cancer 13:377–379
    [Google Scholar]
  36. Sairenji T., Hinuma Y. 1973; Modes of Epstein-Barr virus infection in human floating cell lines. Gann 64:583–590
    [Google Scholar]
  37. Schwaber J. F., Klein G., Ernberg I., Rosen A., Lazarus H., Rosen F. S. 1980; Deficiency of Epstein-Barr virus (EBV) receptors on B lymphocytes from certain patients with common varied agammaglobulinemia. Journal of Immunology 124:2191–2196
    [Google Scholar]
  38. Seigneurin J. M., Vuillaume M., Lenoir G., De Thé G. 1977; Replication of Epstein-Barr virus: ultrastructural and immunofluorescent studies of P3HR-l-superinfected Raji cells. Journal of Virology 24:836–845
    [Google Scholar]
  39. Traul K., Mayyasi S. 1975; Large scale production of Epstein-Barr virus. In Epstein Barr Virus Production, Concentration and Purification IARC Internal Technical Report no. 75 003. pp 81–98 Proceedings of a workshop organized jointly by National Cancer Institute, Bethesda, Md., and IARC, Lyon; France:
    [Google Scholar]
  40. Twomey J. J. 1974; Abnormalities in the mixed lymphocyte reaction during infectious mononucleosis. Journal of Immunology 112:2278–2281
    [Google Scholar]
  41. Yefenof E., Klein G., Jondal M., Oldstone M. B. A. 1976; Surface markers on human B and T lymphocytes. IX. Two color immunofluorescence studies on the association between EBV receptors and complement receptors on the surface of lymphoid cell lines. International Journal of Cancer 17:693–700
    [Google Scholar]
  42. Yefenof E., Klein G., Kvaruuny K. 1977; Relationship between complement activation, complement binding and EBV adsorption by human hematopoietic cell lines. Cellular Immunology 31:225–233
    [Google Scholar]
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