1887

Abstract

SUMMARY

The amino acid sequence of the coat protein of the U2 strain of tobacco mosaic virus (TMV) has been re-examined and completed. Four incorrect residue allocations in the published U2 sequence were identified. These were located in the region corresponding to residues 96 to 105. In addition, the identity of residues 106 to 112 was also determined. This latter region is of particular interest in antigenic studies, since the homologous region 108 to 112 in TMV (common strain) corresponds to an antigenic determinant of the depolymerized coat protein. Tryptic peptide 6 of strain U2 (residues 93 to 113) also contains an antigenic determinant, as shown by its ability to inhibit the reaction between U2 protein and specific antibodies.

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1981-02-01
2024-12-13
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References

  1. Atassi M. Z. 1977; The complete antigenic structure of myoglobin: approaches and conclusions for antigenic structures of proteins. In Immunochemistry of Proteins vol 2 pp 77–176 Edited by Atassi M. Z. New York: Plenum Press;
    [Google Scholar]
  2. Atassi M. Z., Lee C. 1978; The precise and entire antigenic structure of native lysozyme. Biochemical Journal 171:429–434
    [Google Scholar]
  3. Bald J. G., Gumpf D. J., Heick J. 1974; Transition from common tobacco mosaic virus to the Nicotiana glauca form. Virology 59:467–476
    [Google Scholar]
  4. Benjamini E. 1977; Immunochemistry of the tobacco mosaic virus protein. In Immunochemistry of Proteins vol 2 pp 265–310 Edited by Atassi M. Z. New York: Plenum Press;
    [Google Scholar]
  5. Chang J. Y., Brauer D., Wittmann Liebold B. 1978; Micro-sequence analysis of peptides and proteins using 4-N, N-dimethylaminoazobenzene 4′-isothiocyanate phenylisothiocyanate double coupling method. FEBS Letters 93:205–214
    [Google Scholar]
  6. Ginoza W., Atkinson D. E. 1955; Comparison of some physical and chemical properties of eight strains of tobacco mosaic virus. Virology 1:253–260
    [Google Scholar]
  7. Hurrell J. G. R., Smith J. A., Todd P. E., Leach S. J. 1977; Cross-reactivity between mammalian myoglobins: linear vs spatial antigenic determinants. Immunochemistry 14:283–288
    [Google Scholar]
  8. Ibrahimi I. M., Prager E. M., White R. J., Wilson A. C. 1979; Amino acid sequence of California quail lysozyme. Effect of evolutionary substitutions on the antigenic structure of lysozyme. Biochemistry 13:2736–2744
    [Google Scholar]
  9. Knight C. A., Silva D. M., Dahl D., Tsugita A. 1962; Two distinctive strains of tobacco mosaic virus. Virology 16:236–243
    [Google Scholar]
  10. Milton R. C., De L., Van Regenmortel M. H. V. 1979; Immunochemical studies of tobacco mosaic virus,. III. Demonstration of five antigenic regions in the protein subunit. Molecular Immunology 16:179–184
    [Google Scholar]
  11. Milton R. C., De L., Milton S. C. F., Von Wechmar M. B., Van Regenmortel M. H. V. 1980; Immunochemical studies of tobacco mosaic virus. IV. Influence of single amino acid exchanges on the antigenic activity of mutant coat proteins and peptides. Molecular Immunology 17:1205–1212
    [Google Scholar]
  12. Reinbolt J., Tritsch D., Wittmann-Liebold B. 1979; The primary structure of ribosomal protein S7 from E. coli strains K and B. Biochemie 61:501–522
    [Google Scholar]
  13. Rentschler L. 1967; Aminosauresequenzen und physikochemisches Verhalten des Hiillproteins eines Wildstammes des Tabakmosaikvirus. I. Analyse der Primarstruktur (Pos. 62–134) des Hiillproteins vom Wildstam U2. Molecular and General Genetics 100:84–95
    [Google Scholar]
  14. Siegel A., Wildman S. G. 1954; Some natural relationships among strains of tobacco mosaic virus. Phytopathology 44:277–282
    [Google Scholar]
  15. Singer S. J., Bald J. G., Wildman S. G., Owen R. D. 1951; The detection and isolation of naturally occurring strains of tobacco mosaic virus by electrophoresis. Science 114:463–465
    [Google Scholar]
  16. Tsugita A., Gish D. T., Young J., Fraenkel-Conrat H., Knight C. A., Stanley W. M. 1960; The Complete amino acid sequence of the protein of tobacco mosaic virus. Proceedings of the National Academy of Sciences of the United States of America 46:1463–1469
    [Google Scholar]
  17. Udenfriend S., Stein S., Bohlfn P., Dairman W., Leimgruber W., Weigele M. 1972; Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range. Science 178:871–872
    [Google Scholar]
  18. Van Regenmortel M. H. V. 1967; Serological studies on naturally occurring strains and chemically induced mutants of tobacco mosaic virus. Virology 31:467–480
    [Google Scholar]
  19. Van Regenmortel M. H. V. 1972; Electrophoresis. In Principles and Techniques in Plant Virology pp 390–412 Edited by Kado C., Agrawal H. O. New York: Van Nostrand Reinhold;
    [Google Scholar]
  20. Van Regenmortel M. H. V. 1975; Antigenic relationships between strains of tobacco mosaic virus. Virology 64:415–420
    [Google Scholar]
  21. Wetter C., Bernard M. 1977; Identifizierung, Reinigung und serologischer Nachweis von Tabakmosaikvirus und Para-Tabakmosaikvirus aus Zigaretten. Phytopathologische Zeitschrift 90:257–267
    [Google Scholar]
  22. Wittmann H. G. 1965; Die primare Proteinstruktur von Stammen des Tabakmosaikvirus. Teil IV. Amino-säuresequenzen (Pos. 1-61 und 135–158) des Proteins des Tabakmosaikvirusstammes U2. Zeitschrift für Naturforschung 20b:1213–1223
    [Google Scholar]
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