The amino acid sequence of the coat protein of the U2 strain of tobacco mosaic virus (TMV) has been re-examined and completed. Four incorrect residue allocations in the published U2 sequence were identified. These were located in the region corresponding to residues 96 to 105. In addition, the identity of residues 106 to 112 was also determined. This latter region is of particular interest in antigenic studies, since the homologous region 108 to 112 in TMV (common strain) corresponds to an antigenic determinant of the depolymerized coat protein. Tryptic peptide 6 of strain U2 (residues 93 to 113) also contains an antigenic determinant, as shown by its ability to inhibit the reaction between U2 protein and specific antibodies.


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