The Correlation of Fatty Acid Content of Infected Cells and Virions with Newcastle Disease Virus (NDV) Virulence Free

Abstract

SUMMARY

Chick embryo fibroblasts (CEF) infected with virulent strains of Newcastle disease virus (NDV) showed a dramatic increase in total unsaturated fatty acids (UFA). This increase was not seen in cells infected with the avirulent strains of NDV, Sendai virus or influenza A (PR8). The virions of the virulent strains of NDV harvested from the chorioallantoic cavity also had a higher UFA content compared to the avirulent ones. The kinetics of UFA increase in the virulent strains could be correlated with published data on the kinetics of RNA and protein synthesis inhibition, polykaryon formation and membrane permeability changes.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-52-2-355
1981-02-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/52/2/JV0520020355.html?itemId=/content/journal/jgv/10.1099/0022-1317-52-2-355&mimeType=html&fmt=ahah

References

  1. Allan W. H., Borland L. J. 1980; A comparison of the immune response and respiratory stress effect of three clone sizes of virus and three ranges of aerosol particle size using the lentogenic NDV vaccine strain AG68L. Avian Pathology 9:153–162
    [Google Scholar]
  2. Apostolov K. 1980; The effects of iodine on the biological activities of myxoviruses. Journal of Hygiene (in press)
    [Google Scholar]
  3. Apostolov K., Saw A M. I. 1976; Enhancement of haemolysis by Newcastle disease virus (NDV) after pre-treatment with heterophile antibody and complement. Journal of General Virology 33:459–469
    [Google Scholar]
  4. Blenkharn J. I., Apostolov K. 1980; The effect of iodination on the haemolytic property and the fatty acids of Newcastle disease virus. Biochimica et Biophysica Acta 597:358–363
    [Google Scholar]
  5. Chapman D. 1975; Fluidity and phase transitions of cell membranes. Biomembranes 7:1–9
    [Google Scholar]
  6. Chapman D., Quinn P. J. 1976; A method for the modulation of membrane fluidity: homogeneous catalytic hydrogenation of phospholipids and phospholipid-water model bio-membranes. Proceedings of the National Academy of Sciences of the United States of America 73:3971–3975
    [Google Scholar]
  7. Hosaka Y. 1975; Artificial assembly of active envelope particles of HVJ (Sendai virus). In Negative Strand Viruses vol 2 pp 885–903 Edited by Mahy B. W. J., Barry R. D. New York and London: Academic Press;
    [Google Scholar]
  8. Katzman J., Wilson D. F. 1974; Newcastle disease virus-induced plasma membrane damage. Journal of General Virology 24:101–113
    [Google Scholar]
  9. Lancaster J. E., Alexander D. J. 1975; Newcastle disease virus. Canadian Department of Agriculture Monograph No. 11
    [Google Scholar]
  10. Nagai Y., Klenk H.-D., Rott R. 1976; Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus. Virology 72:494–508
    [Google Scholar]
  11. Patzer E. J., Moore N. F., Barenholz Y., Shaw M. J., Wagner R. R. 1978; Lipid organisation of the membrane of vesicular stomatitis virus. Journal of Biological Chemistry 253:4544–4550
    [Google Scholar]
  12. Reeve P., Alexander D. J. 1970; Isopycnic separation of Newcastle disease virus on sucrose gradients using zonal ultracentrifuge rotors. Microbios 5:53–58
    [Google Scholar]
  13. Reeve P., Poste G. 1971; Studies on the cytopathogenicity of Newcastle disease virus: relation between virulence, polykaryocytosis and plaque size. Journal of General Virology 11:17–24
    [Google Scholar]
  14. Sawa M. I. 1979 Biological and biochemical aspects of the membrane of Newcastle disease virus (NDV), with special reference to virulence Ph.D. thesis University of London;
    [Google Scholar]
  15. Scheid A., Choppin P. W. 1974; Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, haemolysis and infectivity by proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology 57:475–490
    [Google Scholar]
  16. Schroit A. J., Gallily R. 1979; Macrophage fatty acid composition and phagocytosis: effect of unsaturation on cellular phagocytic activity. Immunology 36:199–205
    [Google Scholar]
  17. Terry G. M., Ho-Terry L. 1976; Fusion and haemolysis of chick erythrocytes by Newcastle disease virus. Archives of Virology 50:37–44
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-52-2-355
Loading
/content/journal/jgv/10.1099/0022-1317-52-2-355
Loading

Data & Media loading...

Most cited Most Cited RSS feed