The Isolation of Large and Small Plaque Canine Distemper Viruses which Differ in their Neurovirulence for Hamsters Free

Abstract

SUMMARY

Large and small plaque-forming viruses were isolated from the Onderstepoort strain of canine distemper virus (CDV). Small plaque virus, which was released more slowly from infected cells than large plaque virus, readily established persistent infections in Vero cells, whereas large plaque virus required undilute passage to do so. All persistently infected cultures eventually released small plaque virus. No difference was found in the size of polypeptides induced by either plaque-purified viruses or virus released from persistent cultures. Both dilute and undilute passage, large plaque virus produced an acute neurological illness in weanling hamsters, whereas small plaque virus failed to produce any clinical signs of disease for 3 months after inoculation. After this period 50% of the animals infected with small plaque virus showed a general deterioration in their condition and lesions were observed in the brain which resembled those found in cases of large plaque virus infection. Serum-neutralizing antibody titres to CDV rapidly increased after infection with small plaque virus, whereas animals infected with large plaque virus had low or undetectable levels. All hamsters infected with small plaque virus and a small number which survived large plaque virus infection had elevated titres of antibody over a test period of 15 months.

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1981-02-01
2024-03-29
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References

  1. Appel M. J. G. 1978; Reversion to virulence of attenuated canine distemper virus in vivo and in vitro . Journal of General Virology 41:385–393
    [Google Scholar]
  2. Bussell R. H., Karzon D. T. 1965; Canine distemper virus in human and monkey cell cultures. Archiv für die gesamte Vinisforschung 17:183–202
    [Google Scholar]
  3. Campbell J. J., Cosby S. L., Scott J. K., Rima B. K., Martin S. J., Appel M. 1980; A comparison of measles and canine distemper virus polypeptides. Journal of General Virology 48:149–159
    [Google Scholar]
  4. Confer A. W., Kahn D. E., Kaestner A., Krakowka S. 1975; Biological properties of a canine distemper virus isolate associated with demyelinating encephalomyelitis. Infection and Immunity 11:835–844
    [Google Scholar]
  5. Connolly J. H., Allen I. V., Hurwitz L. J., Millar J. H. D. 1967; Measles virus antibody and antigen in subacute sclerosing panencephalitis. Lancet i:542–544
    [Google Scholar]
  6. Gould E. A. 1974; Variants of measles virus. Medical Microbiology and Immunology 160:211–219
    [Google Scholar]
  7. Gould E. A., Linton P. E. 1975; The production of a temperature-sensitive persistent measles virus infection. Journal of General Virology 28:21–28
    [Google Scholar]
  8. Gould E. A., Cosby S. L., Shirodaria P. V. 1976; Salt-dependent haemagglutinating measles virus in SSPE. Journal of General Virology 33:139–142
    [Google Scholar]
  9. Hadlow W. J. 1962; Canine distemper. In Comparative Neuropathology pp 383–384 Edited by Innes J. L. M., Sanders L. Z. New York: Academic Press;
    [Google Scholar]
  10. Hall W. W., Martin S. J., Gould E. A. 1974; Defective interfering particles produced during the replication of measles virus. Medical Microbiology and Immunology 160:155–164
    [Google Scholar]
  11. KarnovSky M. 1965; A formaldehyde-glutaraldehyde fixation of high osmolarity for use in electron microscopy. Journal of Cell Biology 27:137A
    [Google Scholar]
  12. Lincoln S. D., Gorham J. R., Ott R. L., Hegreherg G. A. 1971; Etiologic studies of old dog encephalitis. I.Demonstration of canine distemper viral antigen in the brain of two cases. Veterinary Pathology 8:1–8
    [Google Scholar]
  13. Reculard P., Guillon J. C. 1972; Etude experimentale de quelques souches du virus de la maladie de Carre du chien. Identification et definition des souches varientes. Annates de L’Institut Pasteur 123:477–487
    [Google Scholar]
  14. Reed L. J., Muench H. 1938; A simple method of estimating fifty per cent endpoints. American Journal of Hygiene 27:493–497
    [Google Scholar]
  15. Rima B. K., Martin S. J. 1979; Effect of undiluted passage on the polypeptides of measles virus. Journal of General Virology 44:134–144
    [Google Scholar]
  16. Rima B. K., Davidson W. B., Martin S. J. 1977; The role of defective interfering particles in persistent infection of Vero cells by measles virus. Journal of General Virology 35:89–97
    [Google Scholar]
  17. Rima B. K., Gould E. A., Martin S. J. 1979; A comparison of polypeptides in measles and SSPE virus strains. Journal of General Virology 42:603–608
    [Google Scholar]
  18. Ter Meulen V., Müller D., Käckell Y., Katz M., Meyermann R. 1972; Isolation of infectious measles virus in measles encephalitis. Lancet ii:1172
    [Google Scholar]
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