1887

Abstract

SUMMARY

HA—lipid spheres or ‘virosomes’ were prepared using neutral or negatively charged, but not positively charged, phospholipids. Virosomes were similar in size and shape to native virus particles although the HA subunits were at least twofold less numerous on the virosomes. The HA subunits were attached by their narrow end to the lipid bilayer, and could be removed by digestion with bromelain. However, HA subunits released from intact virus by digestion with bromelain, which removed the hydrophobic tail of the molecule, could not attach to liposomes. Measurements of HA spikes before (mean length 14.2 ± 0.9 nm) and after attachment to liposomes (mean length 13.3 ± 0.7 nm) and examination of freeze-fractured virosomes indicated that the HA did not penetrate deeply into the lipid bilayer. Similarly, HA subunits did not penetrate deeply into the lipid of virus particles. NP and M proteins could be attached to liposomes but could not be visualized by electron microscopy. Virosomes were taken up by Vero cells by viropexis with no evidence of fusion. Incorporation of HA or NP on to virosomes resulted in increased immunogenicity compared to free HA subunits or NP respectively. This adjuvant activity was not apparent in simple mixtures of HA and liposomes. The antibody induced by HA subunits, virions and virosomes reacted similarly with strain-specific (SS) antigenic determinants of the haemagglutinin.

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1981-02-01
2024-04-19
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References

  1. Allison A. C., Gregoriadis G. 1974; Liposomes as immunological adjuvants. Nature, London 252:252
    [Google Scholar]
  2. Almeida J. D., Brand C. M., Edwards D. C., Heath T. D. 1975; Formation of virosomes from influenza subunits and liposomes. Lancet ii:899–901
    [Google Scholar]
  3. Bachi T. 1970; Electromicroscopische untersuchungen uber den infectionszyklus von influenza A - viren in Ehrlich - Aszites - Tumor - Zellen. Pathologia et Microbiologia 36:81–107
    [Google Scholar]
  4. Bangham A. D., Hill M. W., Miller N. G. A. 1974; Preparation and use of liposomes as models of biological membranes. Methods in Membrane Biology 1:1–68
    [Google Scholar]
  5. Brand C. M., Skehel J. J. 1972; Crystalline antigen from influenza virus envelope. Nature, London 238:145–147
    [Google Scholar]
  6. Coon J., Hunter R. 1973; Selective induction of delayed hypersensitivity by a lipid conjugated protein antigen which is localized in thymus dependent lymphoid tissue. Journal of Immunology 110:183–193
    [Google Scholar]
  7. Dales S. 1973; Early events in cell-animal virus interactions. Bacteriological Reviews 37:103–135
    [Google Scholar]
  8. Dimitriadis G. J. 1978; Entrapment of ribonucleic acids in liposomes. FEBS Letters 86:289–293
    [Google Scholar]
  9. Dourmashkin R. R., Tyrrell D. A. J. 1974; Electron microscopic observation of the entry of influenza virus into susceptible cells. Journal of General Virology 24:129–141
    [Google Scholar]
  10. Dubovi E. J., Wagner R. R. 1977; Spatial relationships of the proteins of vesicular stomatitis virus: induction of reversible oligomers by cleavable protein cross-linkers and oxidation. Journal of Virology 22:500–509
    [Google Scholar]
  11. Haywood A. M. 1974; Fusion of sendai virus with model membranes. Journal of Molecular Biology 87:625–628
    [Google Scholar]
  12. Heath T. D., Edwards D. C., Ryman B. E. 1976; The adjuvant property of liposomes. Biochemical Society Transactions 4:129–133
    [Google Scholar]
  13. Helenius A., Fries E., Martenbech J. 1977; Reconstitution of Semliki Forest virus membrane. Journal of Cell Biology 75:866–880
    [Google Scholar]
  14. Hsu M.C., Scheid A., Choppin P. W. 1979; Reconstitution of membranes with individual paramyxovirus glycoproteins and phospholipid in cholate solution. Virology 95:476–491
    [Google Scholar]
  15. Huang R. T. C., Wahn K., Klenk H.D., Rott R. 1979; Association of the envelope glycoproteins of influenza virus with liposomes - a model study on viral envelope assembly. Virology 97:212–217
    [Google Scholar]
  16. Koszinowski H. U., Allen H., Gething M. I., Waterfield M. D., Klenk H. D. 1980; Recognition of viral glycoproteins by influenza A-specific cross-reactive cytolytic T lymphocytes. Journal of Experimental Medicine 151:945–958
    [Google Scholar]
  17. Laver W. G., Valentine R. C. 1969; Morphology of the isolated haemagglutinin and neuraminidase subunits of influenza virus. Virology 38:105–119
    [Google Scholar]
  18. Laver W. G., Downie J. C., Webster R. G. 1974; Studies on antigenic variation in influenza virus. Evidence for multiple antigenic determinants on the haemagglutinin subunits of A/Hong Kong/68 (H3N2) virus and the A/England/72 strains. Virology 59:230–244
    [Google Scholar]
  19. Lenard J. 1978; Virus envelopes and plasma membranes. Annual Review of Biophysics and Bioengineering 7:139–165
    [Google Scholar]
  20. Lenard J., Compans R. W. 1974; The membrane structure of lipid-containing viruses. Biochimica et Biophvsica Acta 344:51–94
    [Google Scholar]
  21. Manesis E. K., Cameron C. H., Gregoriadis G. 1979; Hepatitis B surface antigen containing liposomes enhance humoral and cell mediated immunity to the antigen. FEBS Letters 102:107–111
    [Google Scholar]
  22. Nermut M. V. 1972; Further investigations on the fine structure of influenza virus. Journal of General Virology 17:317–331
    [Google Scholar]
  23. Nermut M. V., Frank H. 1971; Fine structure of influenza A2 (Singapore) as revealed by negative staining and freeze-etching. Journal of General Virology 10:37–51
    [Google Scholar]
  24. Nermut M. V., Williams L. D. 1977; Freeze fracturing of monolayers (capillary layers) of cells, membranes and viruses: some technical considerations. Journal of Microscopy 110:121–132
    [Google Scholar]
  25. Oxford J. S., Schild G. C. 1975; Immunological studies with influenza virus matrix protein. In Negative Strand Viruses vol 2 pp 611–620 Edited by Mahy B. W. J., Barry R. D. New York and London: Academic Press;
    [Google Scholar]
  26. Oxford J. S., Schild G. C. 1977; Quantification of influenza structural proteins using rocket immunoelectrophoresis. Journal of General Virology 38:187–193
    [Google Scholar]
  27. Oxford J. S., Schild G. C., Potter C. W., Jennings R. 1979; The specificity of the anti-haemagglutinin antibody response induced in man by inactivated vaccines and by natural infection. Journal of Hygiene 82:51–61
    [Google Scholar]
  28. Patterson S., Oxford J. S., Dourmashkin R. R. 1979; Studies on the mechanism of influenza virus entry into cells. Journal of General Virology 43:223–229
    [Google Scholar]
  29. Rott R. 1980; Studies on the structure-function relationships of influenza virus glycoproteins. In Structure and Variation in Influenza Virus pp 201–209 Edited by Laver W. G., Air G. Amsterdam; Elsevier/North-Holland:
    [Google Scholar]
  30. Russell S. M., Liew F. T. 1979; T cells primed by influenza virion internal components can co-operate in the antibody response to haemagglutinin. Nature, London 280:147–148
    [Google Scholar]
  31. Schulze I. T. 1973; Structure of influenza virion. Advances in Virus Research 18:1–56
    [Google Scholar]
  32. Siddique W. A., Taylor D. W., Kan S. C., Kramer K., Richmond-Crum S. M., Kotani S., Shiba T., Kusimoto S. 1978; Vaccination of experimental monkeys against Plasmodium falciparum: a possible safe adjuvant. Science 201:1237–1239
    [Google Scholar]
  33. Skehel J. J., Schild G. C. 1971; The polypeptide composition of influenza A viruses. Virology 44:396–408
    [Google Scholar]
  34. Szoka F., Papahadjopoulos D. 1978; Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse-phase evaporation. Proceedings of the National Academy of Sciences of the United States of America 75:4194–4198
    [Google Scholar]
  35. Tyrrell D. A., Heath T. D., Colley C. M., Ryman B. E. 1976; Further aspects of liposomes. Biochimica et Biophysica Acta 457:259–302
    [Google Scholar]
  36. Virelizier J. L., Allison A. C., Schild G. C. 1974; Antibody responses to antigenic determinants of influenza virus haemagglutinin. II. Original antigenic sin: a bone-marrow derived lymphocyte memory phenomenon modulated by thymus-derived lymphocytes. Journal of Experimental Medicine 140:1571–1578
    [Google Scholar]
  37. Waterfield M. D., Espelie K., Elder K. 1979; Structure of the haemagglutinin of influenza virus. British Medical Bulletin 35:57–63
    [Google Scholar]
  38. Wrigley N. G. 1979; Electron microscopy of influenza virus. British Medical Bulletin 35:35–38
    [Google Scholar]
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