A persistent infection of the human MCF-7 cell line (MCF-7RV) was established with the DBS strain of rubella virus at a low multiplicity of infection. Fluorescent antibody staining revealed that 100% of the cells were positive for rubella antigens. The infected cells were refractory to superinfection with vesicular stomatitis virus (VSV) but were susceptible to herpes simplex virus type 2 (HSV-2). No interferon could be detected in infected cell culture fluid, and continuous passage in the presence of antibody did not lead to a decrease in the percentage of infected cells. Virus production in the persistently infected cells represented a 5- to 10-fold increase over primary infection. Plaque assays at 30 and 39 °C of the virus produced at 37 °C revealed the presence of temperature-sensitive (ts) mutants. If MCF-7RV cells were maintained at 30 °C, significant increases in virus production were observed, leading to cytopathic effect and destruction of the monolayer. If maintained at 39 °C, MCF-7RV cells produced less virus and demonstrated normal morphology. These data suggest that the naturally selected population of ts mutants being produced by these cells represents the mechanism by which persistence is maintained.
AtkinsG. J.1979; Establishment of persistent infection in BHK-21 cells by temperature-sensitive mutants of Sindbis virus. Journal of General Virology 45:201–207
JoklikW. K.1977; Mechanisms of establishment and maintenance of persistent infections. In Microbiology - 1977 pp 434–438 Edited by
SchlessingerD.
Washington, D. C.: American Society for Microbiology;
MifuneK.,
DesmyterJ.,
RawlsW. E.1970; Effect of exogenous interferon on rubella virus production in carrier cultures of cells defective in interferon production. Infection and Immunity 2:132–138
SatoM.,
TanakaH.,
YamadaT.,
YamamotoN.1977; Persistent infection of BHK-21/WI-2 cells with rubella virus and characterization of rubella variants. Archives of Virology 54:333–343
SatoM.,
MaedaN.,
UradeM.,
KuribayashiM.,
ShirasunaK.,
YoshidaH.,
MiyazakiT.1978; Persistent infection of primary human cell cultures with rubella variant carrying DNA polymerase activity. Archives of Virology 56:181–187
ShenkT. E.,
KoshelnykK. A.,
StollarV.1974; Temperature-sensitive virus from Aedes albopictus cells chronically infected with Sindbis virus. Journal of Virology 13:439–447
SouleH. D.,
VasquezJ.,
LongA.,
AlbertS.,
BrennanM.1973; A human cell line from a pleural effusion derived from a breast carcinoma. Journal of the National Cancer Institute 51:1409–1413
YoungnerJ. S.1977; Role of temperature-sensitive mutants in persistent infection. In Microbiology-1977 pp 451–455 Edited by
SchlessingerD.
Washington, D. C.: American Society for Microbiology;