1887

Abstract

SUMMARY

Antibodies were raised by immunization of monkeys with extracts of kidney cells separately infected with one of the three poliovirus serotypes. Preparations of antibodies were shown to be strictly type-specific in the neutralization test but formed immune complexes adsorbable on , Cowan I strain cells, with heterotypic as well as homotypic virus-specific polypeptides present in extracts from the virus-infected cells. The presence of intertypic antigenic determinants was demonstrated by this technique on both the non-capsid and capsid poliovirus polypeptides.

Structural variations of poliovirus polypeptides were studied by analysing products of their partial proteolysis. Non-capsid polypeptides encoded in the central portion of the virus genome (polypeptides 5b and X) as well as in the 3′-terminal region (NCVP2 and NCVP4) were found to be highly conserved, whereas capsid polypeptides VP1, VP2, and VP3, which are encoded in the 5′-terminal region of the virus RNA, displayed a much greater variability.

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1981-02-01
2024-04-20
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References

  1. Avrameas S., Ternynck T. 1969; The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistiy 6:53–66
    [Google Scholar]
  2. Brown F. 1979; Structure-function relationships in the picornaviruses. In The Molecular Biology of Picornaviruses pp 49–72 Edited by Perez-Bercoff R. New York and London: Plenum Press;
    [Google Scholar]
  3. Butterworth B. E., Shimshick E. J., Yin F. H. 1976; Association of polioviral RNA polymerase complex with phospholipid membranes. Journal of Virology 19:457–466
    [Google Scholar]
  4. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  5. Čumakov I. M., Lipskaya G. Y., Agol V. I. 1979; Comparative studies on the genomes of some picornaviruses: denaturation mapping of replicative form RNA and electron microscopy of heteroduplex RNA. Virology 92:259–270
    [Google Scholar]
  6. Frisby D. R., Newton C., Carey N. H., Fellner P., Newman J. F. E., Harris T. J. R., Brown F. 1976; Oligonucleotide mapping of picornavirus RNAs by two-dimensional electrophoresis. Virology 71:379–388
    [Google Scholar]
  7. Hummeler K., Hamparian V. V. 1958; Studies on complement fixing antigens of poliomyelitis. 1. Demonstration of type and group-specific antigens in native and heated viral preparations. Journal of Immunology 81:499–505
    [Google Scholar]
  8. Hummeler K., Anderson T. F., Brown R. A. 1962; Identification of poliovirus particles of different antigenicity by specific agglutination as seen in the electron microscope. Virology 16:84–90
    [Google Scholar]
  9. Kessler S. W. 1975; Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. Journal of Immunology 115:1617–1624
    [Google Scholar]
  10. Kew O. M., Pallansch M. A., Omilianowski D. R., Rueckert R. R. 1978; Differential rates of evolution of poliovirus protein. Abstracts of the Fourth International Congress of Virology, Wageningen pp 257
    [Google Scholar]
  11. Kew O. M., Pallansch M. A., Omilianowski D. R., Rueckert R. R. 1980; Changes in three of four coat proteins of oral polio vaccine strain derived from type 1 poliovirus. Journal of Virology 33:256–263
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  13. Le Bouvier O. L. 1959; Poliovirus D and C antigens: their differentiation and measurement by precipitation in agar. British Journal of Experimental Pathology 40:452–463
    [Google Scholar]
  14. Le Bouvier G. L., Schwerdt C. E., Schaffer F. L. 1957; Specific precipitates in agar with purified poliovirus. Virology 4:590–593
    [Google Scholar]
  15. Lee V. F., Wimmer E. 1976; Fingerprinting high molecular weight RNA by two-dimensional electrophoresis: application to poliovirus RNA. Nucleic Acids Research 3:1647–1658
    [Google Scholar]
  16. Lee Y. F., Kitamura N., Nomoto A., Wimmer E. 1979; Sequence studies of poliovirus RNA. IV. Nucleotide sequence complexities of poliovirus type 1, type 2 and two type 1 defective interfering particles RNAs, and fingerprint of the poliovirus type 3 genome. Journal of General Virology 44:311–322
    [Google Scholar]
  17. Medappa K. C., McLean C., Rueckert R. R. 1971; On the structure of rhinovirus 1A. Virology 44:259–270
    [Google Scholar]
  18. Meloen R. H., Rowlands D. J., Brown F. 1979; Comparison of the antibodies elicited by the individual structural polypeptides of foot-and-mouth disease and polioviruses. Journal of General Virology 45:761–763
    [Google Scholar]
  19. Newman J. F., Cartwright B., Doel T. R., Brown F. 1979; Purification and identification of the RNA-dependent RNA polymerase of foot-and-mouth disease virus. Journal of General Virology 45:497–507
    [Google Scholar]
  20. Nomoto A., Kajigaya S., Suzuki K., Imura N. 1979; Possible point mutation sites in LSc, 2ab, poliovirus RNA and a protein covalently linked to the 5′-terminus. Journal of General Virology 45:107–117
    [Google Scholar]
  21. Porter A. G., Fellner P., Black D. N., Rowlands D. J., Harris T. J. R., Brown F. 1978; 3′-Terminal nucleotide sequences in the genome RNA of picornaviruses. Nature, London 276:298–301
    [Google Scholar]
  22. Robson K. J. H., Doel T. R., Gorman B. M., Brown F. 1980; Biochemical aspects of variation in foot-and-mouth disease virus. Journal of General Virology 46:179–193
    [Google Scholar]
  23. Rueckert R. R., Mathews T. J., Kew O. M., Pallansch M., McLean C., Omilianowski D. 1979; Synthesis and processing of picornavirai polypeptides. In The Molecular Biology of Picornaviruses pp 113–125 Edited by Perez-Bercoff R. New York and London: Plenum Press;
    [Google Scholar]
  24. Svitkin Y. V., Gorbalenya A. E., Kazachkov Y. A., Agol V. I. 1979; Encephalomyocarditis virus-specific polypeptide p22 possessing a proteolytic activity. Preliminary mapping on the viral genome. FEBS Letters 108:6–9
    [Google Scholar]
  25. Young N. A. 1973a; Polioviruses, coxsackieviruses, and echoviruses: comparison of the genome by RNA hybridization. journal of Virology 11:832–839
    [Google Scholar]
  26. Young N. A. 1973b; Size of gene sequences shared by polioviruses types 1, 2 and 3. Virology 56:400–403
    [Google Scholar]
  27. Young N. A., Hoyer B. H., Martin M. A. 1968; Polynucleotide sequence homologies among polioviruses. Proceedings of the National Academy of Sciences of the United States of America 61:548–555
    [Google Scholar]
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