1887

Abstract

SUMMARY

We report here an assay for quantifying virus-induced lysis, in the absence of antibody and complement, produced within 2 h after adsorption. This technique makes use of CrO release from cell monolayers pre-incubated overnight with the isotope. The release of Cr is specific for virus-induced lysis and is suppressible by 0.001 -Ca. This assay clearly distinguishes between wild-type Chinese hamster ovary (CHO) cells, clone K and a fusion-resistant mutant (CHO-15B), which was found to be resistant to virus-induced cytolysis. The stability of the association of isotope with monolayers of this cell type under the labelling conditions described makes this technique applicable to the study of the cytolytic effects of virus infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-52-2-259
1981-02-01
2024-12-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/52/2/JV0520020259.html?itemId=/content/journal/jgv/10.1099/0022-1317-52-2-259&mimeType=html&fmt=ahah

References

  1. Alexander D. J., Hewlett G., Reeve P., Poste G. 1973; Studies on the cytopathic effects of Newcastle disease virus: the cytopathogenicity of strain Herts 33 in five cell types. Journal of General Virology 21:323–337
    [Google Scholar]
  2. Allison A. C. 1967; Lysosomes in virus-infected cells. In Perspectives in Virology vol 5: pp 29–62 Edited by Pollard M. New York and London: Academic Press;
    [Google Scholar]
  3. Bader J. P., Morgan H. R. 1961; A comparison of cytopathology caused by myxoviruses. Journal of Immunology 87:80–89
    [Google Scholar]
  4. Bratt M. A., Clavell L. A. 1972; Hemolytic interaction of Newcastle disease virus and chicken erythrocytes. I.Quantitative comparison procedure. Applied Microbiology 23:454–460
    [Google Scholar]
  5. Bratt M. A., G Allaher W. R. 1969; Preliminary analysis of the requirements for fusion from within and fusion from without by Newcastle disease virus. Proceedings of the National Academy of Sciences of the United States of America 64:536–543
    [Google Scholar]
  6. Clavell L. A., Bratt M. A. 1972; Hemolytic interaction of Newcastle disease virus and chicken erythrocytes. I.Determining factors. Applied Microbiology 23:461–470
    [Google Scholar]
  7. Compans R. W., Holmes K. V., Dales S., Choppes P. W. 1966; An electron microscope study of moderate and virulent virus-cell interactions of the parainfluenza virus SV5 . Virology 30:411–426
    [Google Scholar]
  8. Doermann A. H. 1948; Lysis and lysis inhibition with Escherichia coli bacteriophage. Journal of Bacteriology 55:257–276
    [Google Scholar]
  9. Dulbecco R., Vogt M. 1954; Plaque formation and isolation of pure lines of poliomyelitis virus. Journal of Experimental Medicine 99:167–182
    [Google Scholar]
  10. Gallaher W. R., Levitan D. B., Blough H. A. 1973; Effect of 2-deoxy-D-glucose on cell fusion induced by Newcastle disease and herpes simplex viruses. Virology 55:193–201
    [Google Scholar]
  11. Glorioso J. C., Wilson L. A., Fenger T. W., Smith J. W. 1978; Complement-mediated cytolysis of HSV-1 and HSV-2 infected cells: plasma membrane antigens reactive with type-specific and cross-reactive antibody. Journal of General Virology 40:443–454
    [Google Scholar]
  12. Marcus P. I., Sekellick M. J. 1974; Cell killing by viruses. I. Comparison of cell-killing, plaque-forming, and defective-interfering particles of vesicular stomatitis virus. Virology 57:321–338
    [Google Scholar]
  13. Nagai Y., Klenk H. D., Rott R. 1976; Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus. Virology 72:494–508
    [Google Scholar]
  14. Okada Y., Murayama F. 1966; Requirement of calcium ions for the cell fusion reaction of animal cells by HVJ. Experimental Cell Research 44:527–551
    [Google Scholar]
  15. Polos P. G., Gallaher W. R. 1979; Insensitivity of a ricin-resistant mutant of Chinese hamster ovary cells to fusion induced by Newcastle disease virus. Journal of Virology 30:69–75
    [Google Scholar]
  16. Sato T., Tomita Y., Kuwata T. 1980; Inhibition by interferon of Sendai virus cytolysis. Infection and Immunity 28:6–10
    [Google Scholar]
  17. Scaife J. M., Brohee M. A. 1967; A quantitative measurement of cell damage by measurement of 5lCr-binding. Experimental Cell Research 46:612–615
    [Google Scholar]
  18. Scheid A., Choppin P. W. 1974; Identification of biological activities of paramyxovirus glycoproteins: activation of cell fusion, hemolysis and infectivity by proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology 57:475–490
    [Google Scholar]
  19. Smith J. W., Adam E., Melnick J. L., Rawls W. E. 1972; Use of the 51Cr release test to demonstrate patterns of antibody response in humans to herpesvirus types 1 and 2. Journal of Immunology 109:554–564
    [Google Scholar]
  20. Tennant J. R. 1964; Evaluation of the trypan blue technique for the determination of cell viability. Transplantation 2:685–694
    [Google Scholar]
  21. Toyama S., Toyama S., Uetake H. 1977; Altered cell fusion capacity in lines of KB cells resistant to Sendai-virus-induced cytolysis. Virology 76:503–515
    [Google Scholar]
  22. Wilson L. A., Amos D. B. 1972; Subcellular location of HLA antigens. Tissue Antigens 2:105–111
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-52-2-259
Loading
/content/journal/jgv/10.1099/0022-1317-52-2-259
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error