1887

Abstract

SUMMARY

A DNA-binding antigen (HATNA) was demonstrated in 7 out of 14 cell lines carrying (HVA) by the acid-fixed nuclear-binding technique. The seven HATNA-positive lines had means of 95, 96, 103, 177, 240, 343 and 326 virus genome equivalents/cell. For the seven HATNA-negative lines, the figures were 4, 8, 10, 33, 39, 72 and 110. This indicates a relationship between the number of HVA genome equivalents/cell and the detectability of HATNA. This association was independent of the virus producer status of the lines.

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1981-02-01
2024-12-07
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References

  1. Daniel M. D., Rabin H., Barahona H. H., Melendez L. V. 1971; Herpesvirus saimiri. III. Plaque formation under multi-agar methyl cellulose and starch overlays. Proceedings of the Society for Experimental Biology and Medicine 136 12:589–597
    [Google Scholar]
  2. Epstein M. A., Achong Y. M., Barr B., Zajac B., Henle G., Henle W. 1966; Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji). Journal of the National Cancer Institute 37:547–559
    [Google Scholar]
  3. Ernberg I., Andersson-Anvret M., Klein G., Lundin L., Killander D. 1977; Relationship between amount of Epstein-Barr virus determined nuclear antigen per cell and number of EBV copies per cell. Nature, London 266:269–271
    [Google Scholar]
  4. Falk L., Nigida S. M., Deinhardt F., Wolfe L. G., Cooper R. W., Hernandez Camacho J. I. 1974a; Herpesvirus ateles: properties of an oncogenic herpesvirus isolated from circulation lymphocytes of spider monkeys (ateles sp.). International Journal of Cancer 14:473–482
    [Google Scholar]
  5. Falk L., Wright J., Wolfe L., Deinhardt F. 1974b; Herpesvirus ateles: transformation in vitro of marmoset splenic lymphocytes. International Journal of Cancer 14:244–251
    [Google Scholar]
  6. Falk L., Wolfe L., Deinhardt F., Paciga J., Dombos L., Klein G., Henle W., Henle G. 1974c; Epstein-Barr virus transformation of non-human primate lymphocytes in vitro . International Journal of Cancer 13:363–376
    [Google Scholar]
  7. Falk L., Johnson D. R., Deinhardt F. 1978; Transformation of marmoset lymphocytes in vitro with Herpesvirus ateles . International Journal of Cancer 21:652–657
    [Google Scholar]
  8. Fleckenstein B., Bornkamm G. W., Ludwig H. 1975; Repetitive sequences in complete and defective genomes of Herpesvirus saimiri . Journal of Virology 15:398
    [Google Scholar]
  9. Fleckenstein B., Bornkamm G. W., Mulder C., Werner F. J., Daniel M. D., Falk L., Delius H. 1978; Herpesvirus ateles DNA and its homology with Herpesvirus saimiri nucleic acid. Journal of Virology 25:361–373
    [Google Scholar]
  10. Klein G., Luka J., Zeuthen J. 1979; Epstein-Barr virus (EBV)-induced transformation and the role of the nuclear antigen (EBNA). Cold Spring Harbor Symposia on Quantitative Biology (in press)
    [Google Scholar]
  11. Luka J., Siegert W., Klein G. 1977; Solubilization of the Epstein-Barr virus determined nuclear antigen and its characterization as a DNA-binding protein. Journal of Virology 22:1–8
    [Google Scholar]
  12. Luka J., Lindahl T., Klein G. 1978; Purification of the Epstein-Barr virus determined nuclear antigen from Epstein-Barr virus-transformed human lymphoid cell lines. Journal of Virology 27:604–611
    [Google Scholar]
  13. Ohno S., Luka J., Klein G., Daniel M. D. 1979; Detection of a nuclear antigen in Herpesvirus ateles carrying marmoset lines by the acid-fixed nuclear binding technique. Proceedings of the National Academy of Sciences of the United States of America 76:2042–2046
    [Google Scholar]
  14. Reed S. I., Ferguson J., Davis R. W., Stark G. R. 1975; T antigen binds to simian virus 40 DNA at the origin of replication. Proceedings of the National Academy of Sciences of the United States of America 72:1605–1609
    [Google Scholar]
  15. Reedman B. M., Klein G. 1973; Cellular localization of an Epstein-Barr virus (EBV) associated complement fixing antigen in producer and non-producer lymphoblastoid cell lines. International Journal of Cancer 11:499–530
    [Google Scholar]
  16. Shapiro I. M., Luka J., Andersson Anvret M., Klein G. 1979; Relationship between Epstein-Barr vims genome number, amount of nuclear antigen, and early antigen inducibility in diploid and tetraploid lymphoma cells of related origin. Intervirology 12:19–25
    [Google Scholar]
  17. Tegtmeyer P. 1975; Function of simian virus 40 gene A in transforming infection. Journal of Virology 15:613–618
    [Google Scholar]
  18. Tjian R., Fey G., Graessmann A. 1978; Biological activity of purified simian virus 40 T antigen proteins. Proceedings of the National Academy of Sciences of the United States of America 75:1279–1283
    [Google Scholar]
  19. Wolf H. 1975; A procedure for simultaneous preparation of large amounts of DNA and RNA by the use of potassium iodide gradients. Analytical Biochemistry 58:505–511
    [Google Scholar]
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