1887

Abstract

SUMMARY

Vaccinia virus mRNAs carry the cap structure mGpppAm- or mGpppGm- at the 5′-terminus, which is synthesized by a series of RNA polymerase and capping enzymes contained in the virus particle. The process of the cap formation at the 5′-terminus of mRNA was studied using an system under similar conditions to those of vaccinia virus multiplication in its host cell. After adding a methyl-group donor, methyl-H--adenosylmethionine, the oligonucleotides, which were the synthesized 5′-terminal part of mRNA, were isolated from the RNA-synthesizing virion at appropriate time intervals, and were analysed. The 5′-5′ confronting nucleotides with 2′--methylation, GpppAm and GpppGm, were found with the completed cap structure, mGpppAm and mGpppGm. The confronting nucleotides with only 7-methyl guanine as a methylated component, mGpppA and mGpppG, were not detected at any incubation time, and it was concluded that methylation at the 2′-position of the 5′-terminal purine nucleoside of mRNA precedes methylation of the 7-position of the blocking guanosine. This result is different from that obtained using the enzymes isolated from vaccinia virus (Moss ., 1976) and also from the results obtained using other kinds of virus particles, which carry RNA polymerase and capping enzymes. These differences may be due to the specific organization of a series of capping enzymes and RNA polymerase in each virus particle.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-52-1-49
1981-01-01
2022-11-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/52/1/JV0520010049.html?itemId=/content/journal/jgv/10.1099/0022-1317-52-1-49&mimeType=html&fmt=ahah

References

  1. Banerjee A. K., Abraham O., Colonno R. J. 1977; Vesicular stomatitis virus: mode of transcription. Journal of General Virology 34:1–8
    [Google Scholar]
  2. Furuichi Y. 1974; ‘Methylation-coupled’ transcription by virus-associated transcriptase of cytoplasmic polyhedrosis virus containing double-stranded RNA. Nucleic Acids Research 1:809–822
    [Google Scholar]
  3. Furuichi Y., Miura K. 1975; A blocked structure at the 5′-terminus of mRNA from cytoplasmic polyhedrosis virus. Nature, London 253:374–375
    [Google Scholar]
  4. Furuichi Y., Muthukrishnan S., Tomasz J., Shatkin A. J. 1976; Caps in eukaryotic mRNAs: mechanism of formation of reovirus mRNA 5′-terminal m7GpppGm-C. Progress in Nucleic Acid Research and Molecular Biology 19:3–38
    [Google Scholar]
  5. Haines J. A., Reese C. B., Todd L. 1962; The methylation of guanosine and related compounds with diazomethane. Journal of the Chemical Society5281–5288
    [Google Scholar]
  6. Kates J., Beeson J. 1970; Ribonucleic acid synthesis in vaccinia virus. I. The mechanism of synthesis and release of RNA in vaccinia cores. Journal of Molecular Biology 50:1–18
    [Google Scholar]
  7. Miura K., Furuichi Y., Shimotohno K., Urushibara T., Watanabe K., Sugiura M. 1975a; Modification at the 5′-terminus of messenger RNA strand. Les Colloques de I’Institut National de la Sante et de la Recherche Medicate 47:153–160
    [Google Scholar]
  8. Miura K., Furuichi Y., Shimotohno K., Urushibara T., Sugiura M. 1975b; Synthesis of viral messenger RNA carrying a unique modified structure. Proceedings of the 10th Meeting of the Federation of European Biochemical Societies95–108
    [Google Scholar]
  9. Moss B., Gershowita A., Wei C. M., Boone R. 1976; Formation of the guanylylated and methylated 5′-terminus of vaccinia virus mRNA. Virology 72:341–351
    [Google Scholar]
  10. Shimotohno K., Miura K. 1976; The process of formation of the 5′-terminal modified structure in messenger RNA of cytoplasmic polyhedrosis virus. FEBS Letters 64:204–208
    [Google Scholar]
  11. Urushibara T., Furuichi Y., Nishimura C., Miura K. 1975; A modified structure at the 5′-terminus of mRNA of vaccinia virus. FEBS Letters 49:385–389
    [Google Scholar]
  12. Venkatesan S., Gershowitz A., Moss B. 1980; Modification of the 5′ end of mRNA. Journal of Biological Chemistry 255:903–908
    [Google Scholar]
  13. Wei C. M., Moss B. 1975; Methylated nucleotides block 5′-terminus of vaccinia virus messenger RNA. Proceedings of the National Academy of Sciences of the United States of America 72:318–322
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-52-1-49
Loading
/content/journal/jgv/10.1099/0022-1317-52-1-49
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error